################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 240 GTAC GT^AC AocI 34 CCTGAGG CC^T[ACGT]AGG Aor51HI 248 AGCGCT AGC^GCT BanII 55 GAGCTC G[GA]GC[CT]^C BglI 172 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 419 AGATCT A^GATCT BsaI 321 GGTCTC GGTCTC(1/5) BsaWI 425 ACCGGA [AT]^CCGG[AT] BseRI 37 GAGGAG GAGGAG(10/8) BsiEI 255 CGACCG CG[GA][CT]^CG BsmAI 322 GTCTC GTCTC(1/5) BsrDI 360 CATTGC GCAATG(2/0) BsrFI 26 ACCGGC [GA]^CCGG[CT] BstYI 419 AGATCT [GA]^GATC[CT] Bsu36I 34 CCTGAGG CC^T[ACGT]AGG Cfr10I 26 ACCGGC [GA]^CCGG[CT] DsaI 182 CCGCGG C^C[GA][CT]GG EaeI 179 TGGCCG [CT]^GGCC[GA] EarI 220 CTCTTC CTCTTC(1/4) Eco47III 248 AGCGCT AGC^GCT Eco81I 34 CCTGAGG CC^T[ACGT]AGG HphI 89 TCACC GGTGA(8/7) Ksp632I 220 CTCTTC CTCTTC(1/4) KspI 182 CCGCGG CCGC^GG MaeIII 337 GTGAC ^GT[ACGT]AC MflI 419 AGATCT [GA]^GATC[CT] MseI 263 TTAA T^TAA NlaIII 177 CATG CATG^ PaeR7I 11 CTCGAG C^TCGAG PvuII 332 CAGCTG CAG^CTG RsaI 240 GTAC GT^AC SacI 55 GAGCTC GAGCT^C SacII 182 CCGCGG CCGC^GG ScaI 239 AGTACT AGT^ACT SmlI 11 CTCGAG C^T[CT][GA]AG StuI 80 AGGCCT AGG^CCT Tru9I 263 TTAA T^TAA Tsp45I 337 GTGAC ^GT[GC]AC Tsp509I 261 AATT ^AATT XhoI 11 CTCGAG C^TCGAG XhoII 419 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 69 CGCG CG^CG 183 CGCG AspHI 8 GTGCTC G[AT]GC[AT]^C 55 GAGCTC AvaII 24 GGACC G^G[AT]CC 459 GGACC BanI 29 GGCACC G^G[CT][GA]CC 438 GGCACC BmyI 8 GTGCTC G[AGT]GC[ACT]^C 55 GAGCTC BpmI 58 CTCCAG CTGGAG(16/14) 193 CTGGAG BsiHKAI 8 GTGCTC G[AT]GC[AT]^C 55 GAGCTC Bsp1286I 8 GTGCTC G[AGT]GC[ACT]^C 55 GAGCTC BstF5I 105 CATCC GGATG(2/0) 384 CATCC BstUI 69 CGCG CG^CG 183 CGCG Cac8I 123 GCTGGC GC[ACGT]^[ACGT]GC 448 GCCAGC DpnII 16 GATC ^GATC 420 GATC FokI 105 CATCC GGATG(9/13) 384 CATCC HaeII 186 GGCGCT [GA]GCGC^[CT] 248 AGCGCT MboI 16 GATC ^GATC 420 GATC MspA1I 182 CCGCGG C[AC]G^C[GT]G 332 CAGCTG MvnI 69 CGCG CG^CG 183 CGCG NdeII 16 GATC ^GATC 420 GATC Sau3AI 16 GATC ^GATC 420 GATC SmaI 344 CCCGGG CCC^GGG 442 CCCGGG TaqI 12 TCGA T^CGA 254 TCGA TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^ 297 CAGTG TthHB8I 12 TCGA T^CGA 254 TCGA XmaCI 344 CCCGGG C^CCGGG 442 CCCGGG XmaI 344 CCCGGG C^CCGGG 442 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AvaI BbvI BcnI BsaJI BsiYI BslI BsmFI BsoBI BsrI BssKI BstNI CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI SfaNI TseI