(Linear) (Six-base) MAPSORT of: KIEE1660.seq Check: 352 from: 1 to: 531 KIEE1660.orf With 182 enzymes: SgfI * July 15, 2010 18:44 .. AlwNI CAG_nnn'CTG Cuts at: 0 104 116 312 531 Size: 104 12 196 219 Fragments arranged by size: 219 196 104 12 AvaI C'yCGr_G Cuts at: 0 377 531 Size: 377 154 BanI G'GyrC_C Cuts at: 0 448 531 Size: 448 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 408 531 Size: 408 123 BbvCI CC'TCA_GC Cuts at: 0 154 531 Size: 154 377 BglI GCCn_nnn'nGGC Cuts at: 0 195 531 Size: 195 336 Bme1580I G_kGCm'C Cuts at: 0 337 451 531 Size: 337 114 80 Fragments arranged by size: 337 114 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 490 531 Size: 288 202 41 Fragments arranged by size: 288 202 41 Bpu10I CC'TnA_GC Cuts at: 0 154 495 531 Size: 154 341 36 Fragments arranged by size: 341 154 36 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 531 Size: 160 371 BseYI C'CCAG_C Cuts at: 0 109 121 531 Size: 109 12 410 Fragments arranged by size: 410 109 12 BsiHKAI G_wGCw'C Cuts at: 0 166 531 Size: 166 365 BsmI GAATG_Cn' Cuts at: 0 64 531 Size: 64 467 Bsp1286I G_dGCh'C Cuts at: 0 166 337 451 531 Size: 166 171 114 80 Fragments arranged by size: 171 166 114 80 BsrDI GCAATG_nn' Cuts at: 0 80 531 Size: 80 451 BsrFI r'CCGG_y Cuts at: 0 296 531 Size: 296 235 BtgI C'CryG_G Cuts at: 0 435 531 Size: 435 96 BtsI GCAGTG_nn' Cuts at: 0 339 531 Size: 339 192 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 307 342 531 Size: 307 35 189 Fragments arranged by size: 307 189 35 EaeI y'GGCC_r Cuts at: 0 427 531 Size: 427 104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 490 531 Size: 288 202 41 Fragments arranged by size: 288 202 41 EcoO109I rG'GnC_Cy Cuts at: 0 309 531 Size: 309 222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 260 531 Size: 260 271 MspA1I CmG'CkG Cuts at: 0 113 531 Size: 113 418 NcoI C'CATG_G Cuts at: 0 435 531 Size: 435 96 PflMI CCAn_nnn'nTGG Cuts at: 0 436 531 Size: 436 95 PpuMI rG'GwC_Cy Cuts at: 0 309 531 Size: 309 222 PvuII CAG'CTG Cuts at: 0 113 531 Size: 113 418 SfcI C'TryA_G Cuts at: 0 52 96 219 352 420 531 Size: 52 44 123 133 68 111 Fragments arranged by size: 133 123 111 68 52 44 SmaI CCC'GGG Cuts at: 0 379 531 Size: 379 152 StyI C'CwwG_G Cuts at: 0 435 531 Size: 435 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 224 466 531 Size: 224 242 65 Fragments arranged by size: 242 224 65 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 531 Size: 199 32 300 Fragments arranged by size: 300 199 32 XmaI C'CCGG_G Cuts at: 0 377 531 Size: 377 154 Enzymes that do cut: AlwNI AvaI BanI BbsI BbvCI BglI Bme1580I BpmI Bpu10I BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BtgI BtsI CspCI EaeI Eco57MI EcoO109I MmeI MspA1I NcoI PflMI PpuMI PvuII SfcI SmaI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI