(Circular) (Six-base) MAPSORT of: pFN21AE4133.seq Check: 8511 from: 1 to: 6319 pFN21AE4133 6319 bp With 182 enzymes: SgfI * March 28, 2012 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2452 2452 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1996 2253 3179 3474 4382 5766 6034 1632 Size: 364 257 926 295 908 1384 268 1917 Fragments arranged by size: 1917 1384 926 908 364 295 268 257 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2474 1184 Size: 1290 5029 AlwNI CAG_nnn'CTG Cuts at: 2061 2150 2188 2813 4062 5634 2061 Size: 89 38 625 1249 1572 2746 Fragments arranged by size: 2746 1572 1249 625 89 38 ApaI G_GGCC'C Cuts at: 3352 3352 Size: 6319 ApaLI G'TGCA_C Cuts at: 2560 4376 5532 2560 Size: 1816 1156 3347 Fragments arranged by size: 3347 1816 1156 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2396 3644 1870 Size: 73 453 1248 4545 Fragments arranged by size: 4545 1248 453 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3401 3640 5095 618 Size: 325 217 627 1614 239 1455 1842 Fragments arranged by size: 1842 1614 1455 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2177 3352 3369 3638 1391 Size: 786 1175 17 269 4072 Fragments arranged by size: 4072 1175 786 269 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5348 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2411 3098 4930 Size: 107 122 71 631 605 739 687 1832 Cuts at: 4930 136 Size: 1525 Fragments arranged by size: 1832 1525 739 687 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 2353 2564 3352 4380 5536 1790 Size: 563 211 788 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 788 563 211 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 2832 4050 Size: 416 53 83 399 24 617 798 1218 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 1218 958 798 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2856 2856 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2878 4979 1239 Size: 1028 611 2101 2579 Fragments arranged by size: 2579 2101 1028 611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3020 4444 5309 5607 2369 Size: 651 1424 865 298 3081 Fragments arranged by size: 3081 1424 865 651 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2617 4110 493 Size: 711 1413 1493 2702 Fragments arranged by size: 2702 1493 1413 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 3081 3111 3119 Size: 30 165 30 687 30 961 30 8 Cuts at: 3119 3149 1178 Size: 30 4348 Fragments arranged by size: 4348 961 687 165 30 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 2384 2447 2489 3185 1186 Size: 929 269 63 42 696 4320 Fragments arranged by size: 4320 929 696 269 63 42 BseYI C'CCAG_C Cuts at: 2129 2488 2527 3351 3459 5522 6018 2129 Size: 359 39 824 108 2063 496 2430 Fragments arranged by size: 2430 2063 824 496 359 108 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 2652 1163 Size: 573 744 172 4830 Fragments arranged by size: 4830 744 573 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2564 2822 3369 4380 4465 5536 2177 Size: 387 258 547 1011 85 1071 2960 Fragments arranged by size: 2960 1071 1011 547 387 258 85 BsmI GAATG_Cn' Cuts at: 3532 3830 3923 3532 Size: 298 93 5928 Fragments arranged by size: 5928 298 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 2285 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2353 2564 2822 3352 3369 3638 Size: 399 387 176 211 258 530 17 269 Cuts at: 3638 4380 4465 5536 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 387 269 258 211 176 85 17 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 3225 4208 5938 3225 Size: 983 1730 3606 Fragments arranged by size: 3606 1730 983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 3575 3674 844 Size: 1608 1123 99 3489 Fragments arranged by size: 3489 1608 1123 99 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 2188 794 Size: 1394 4925 BstBI TT'CG_AA Cuts at: 3184 5123 3184 Size: 1939 4380 BstEII G'GTnAC_C Cuts at: 1184 2297 3049 1184 Size: 1113 752 4454 Fragments arranged by size: 4454 1113 752 BstXI CCAn_nnnn'nTGG Cuts at: 2689 3321 2689 Size: 632 5687 BstYI r'GATC_y Cuts at: 1972 2908 3649 4016 4401 4418 5870 5956 5968 Size: 936 741 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 936 741 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2303 2367 2757 3243 2303 Size: 64 390 486 5379 Fragments arranged by size: 5379 486 390 64 BtgI C'CryG_G Cuts at: 513 1065 2424 513 Size: 552 1359 4408 Fragments arranged by size: 4408 1359 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2226 3835 4629 4649 2226 Size: 1609 794 20 3896 Fragments arranged by size: 3896 1609 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3239 3274 529 Size: 35 2675 35 3574 Fragments arranged by size: 3574 2675 35 35 DraI TTT'AAA Cuts at: 3151 3623 3151 Size: 472 5847 DraIII CAC_nnn'GTG Cuts at: 2938 2938 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284 Size: 54 1425 50 275 18 1936 889 1629 Cuts at: 6284 8 Size: 43 Fragments arranged by size: 1936 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2424 2796 3288 3495 4249 1353 Size: 680 391 372 492 207 754 3423 Fragments arranged by size: 3423 754 680 492 391 372 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2175 3367 2175 Size: 1192 5127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2253 2267 2878 3179 3474 4382 Size: 393 364 257 14 611 301 295 908 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 908 787 611 597 393 364 301 295 268 257 14 EcoO109I rG'GnC_Cy Cuts at: 2507 2810 2507 Size: 303 6016 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 3197 3197 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 3358 5466 1678 Size: 1680 2108 2531 Fragments arranged by size: 2531 2108 1680 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2634 2666 3207 3239 3374 Size: 32 135 32 1062 32 541 32 135 Cuts at: 3374 3406 3569 3601 4960 4992 5034 5066 1373 Size: 32 163 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 1062 541 163 135 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 3381 4277 4636 4795 518 Size: 648 978 1237 896 359 159 2042 Fragments arranged by size: 2042 1237 978 896 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2454 2488 2701 2777 3735 4412 5560 Size: 600 248 34 213 76 958 677 1148 Cuts at: 5560 5805 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 958 677 600 248 245 213 76 34 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5767 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2195 2548 2767 3677 5222 1132 Size: 97 966 353 219 910 1545 2229 Fragments arranged by size: 2229 1545 966 910 353 219 97 PasI CC'CwG_GG Cuts at: 1348 2110 2555 2813 1348 Size: 762 445 258 4854 Fragments arranged by size: 4854 762 445 258 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2729 1108 Size: 1621 4698 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3252 1613 Size: 1639 4680 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 3348 3348 Size: 6319 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2628 2660 2628 Size: 32 6287 PstI C_TGCA'G Cuts at: 830 3671 830 Size: 2841 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2488 2488 Size: 6319 SacI G_AGCT'C Cuts at: 2177 3369 2177 Size: 1192 5127 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 3667 4802 5483 5674 826 Size: 219 2622 1135 681 191 1471 Fragments arranged by size: 2622 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2398 3646 1872 Size: 526 1248 4545 Fragments arranged by size: 4545 1248 526 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3035 4423 5324 5586 820 Size: 197 926 441 651 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 651 441 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 3004 4243 5 Size: 47 2952 1239 2081 Fragments arranged by size: 2952 2081 1239 47 StuI AGG'CCT Cuts at: 1517 3156 1517 Size: 1639 4680 StyI C'CwwG_G Cuts at: 513 1065 2796 3758 513 Size: 552 1731 962 3074 Fragments arranged by size: 3074 1731 962 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 3313 4346 4363 4516 4701 5146 Size: 1255 705 1028 1033 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 1498 1255 1033 1028 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2635 2914 4565 Size: 274 80 33 51 1443 658 279 1651 Cuts at: 4565 6277 96 Size: 1712 138 Fragments arranged by size: 1712 1651 1443 658 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 3081 3113 6279 6311 2420 Size: 32 629 32 3166 32 2428 Fragments arranged by size: 3166 2428 629 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2396 3644 1870 Size: 526 1248 4545 Fragments arranged by size: 4545 1248 526 XmnI GAAnn'nnTTC Cuts at: 3587 4448 3587 Size: 861 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI