Restriction map for
pFN21AE5159
(Circular) (Six-base) MAPSORT of: pFN21AE5159.seq Check: 1358 from: 1 to: 5185
pFN21AE5159 5185 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2995 278
Size: 53 83 186 936 1459 2468
Fragments arranged by size:
2468 1459 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2263 2528 1937
Size: 326 265 4594
Fragments arranged by size:
4594 326 265
Acc65I G'GTAC_C
Cuts at: 2506 2506
Size: 5185
AclI AA'CG_TT
Cuts at: 1563 3312 3685 1563
Size: 1749 373 3063
Fragments arranged by size:
3063 1749 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3248 4632 4900 1632
Size: 1616 1384 268 1917
Fragments arranged by size:
1917 1616 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4988
AflIII A'CryG_T
Cuts at: 1203 4084 5061 1203
Size: 2881 977 1327
Fragments arranged by size:
2881 1327 977
AgeI A'CCGG_T
Cuts at: 4016 4016
Size: 5185
AhdI GACnn_n'nnGTC
Cuts at: 3914 3914
Size: 5185
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5185
AlwNI CAG_nnn'CTG
Cuts at: 2928 4500 2928
Size: 1572 3613
ApaLI G'TGCA_C
Cuts at: 2352 3242 4398 2352
Size: 890 1156 3139
Fragments arranged by size:
3139 1156 890
ApoI r'AATT_y
Cuts at: 1493 2031 2494 2720 2872 4981 1493
Size: 538 463 226 152 2109 1697
Fragments arranged by size:
2109 1697 538 463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3739 160
Size: 872 2707 1606
Fragments arranged by size:
2707 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5185
AvaI C'yCGr_G
Cuts at: 1870 1943 2510 1870
Size: 73 567 4545
Fragments arranged by size:
4545 567 73
BamHI G'GATC_C
Cuts at: 2515 2882 2515
Size: 367 4818
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 2506 3961 618
Size: 325 217 627 530 189 1455 1842
Fragments arranged by size:
1842 1455 627 530 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2075 2110 2504 1391
Size: 684 35 394 4072
Fragments arranged by size:
4072 684 394 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2175 2209 3376 3410 1903
Size: 34 238 34 1167 34 3678
Fragments arranged by size:
3678 1167 238 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2460 2467 3077 4287 2460
Size: 7 610 1210 3358
Fragments arranged by size:
3358 1210 610 7
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5185
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3796 136
Size: 107 122 71 631 605 2124 1525
Fragments arranged by size:
2124 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5180 5180
Size: 5185
BlpI GC'TnA_GC
Cuts at: 2602 2602
Size: 5185
Bme1580I G_kGCm'C
Cuts at: 1790 2356 3246 4402 1790
Size: 566 890 1156 2573
Fragments arranged by size:
2573 1156 890 566
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5185
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 3874 442
Size: 416 53 83 399 24 1499 958 1753
Fragments arranged by size:
1753 1499 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3845 1239
Size: 2606 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3310 4175 4473 3310
Size: 865 298 4022
Fragments arranged by size:
4022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3848 882
Size: 617 2349 2219
Fragments arranged by size:
2349 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2976 493
Size: 711 1772 2702
Fragments arranged by size:
2702 1772 711
BsaBI GATnn'nnATC
Cuts at: 5179 5179
Size: 5185
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2992 3374 4009 275
Size: 53 83 186 936 1459 382 635 1451
Fragments arranged by size:
1459 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3618 4016 4290 4437 4848 3618
Size: 398 274 147 411 3955
Fragments arranged by size:
3955 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4960
Fragments arranged by size:
4960 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5185
BseYI C'CCAG_C
Cuts at: 4388 4884 4388
Size: 496 4689
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4612
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2635 3396 4424 1540
Size: 275 186 634 761 1028 2301
Fragments arranged by size:
2301 1028 761 634 275 186
BsiHKAI G_wGCw'C
Cuts at: 2356 3246 3331 4402 2356
Size: 890 85 1071 3139
Fragments arranged by size:
3139 1071 890 85
BsmI GAATG_Cn'
Cuts at: 2696 2789 2696
Size: 93 5092
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2110 2356 2504 3246 3331 4402
Size: 399 285 35 246 148 742 85 1071
Cuts at: 4402 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 285 246 148 85
35
BspEI T'CCGG_A
Cuts at: 4848 4848
Size: 5185
BspHI T'CATG_A
Cuts at: 3074 4804 3074
Size: 1730 3455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2540 844
Size: 1520 176 3489
Fragments arranged by size:
3489 1520 176
BsrBI CCG'CTC
Cuts at: 1394 1823 3072 1394
Size: 429 1249 3507
Fragments arranged by size:
3507 1249 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2157 2169 3680 3854 65
Size: 1183 909 12 1511 174 1396
Fragments arranged by size:
1511 1396 1183 909 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 3829 4016 1700
Size: 110 2019 187 2869
Fragments arranged by size:
2869 2019 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5185
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5185
BssSI C'ACGA_G
Cuts at: 3245 4257 3245
Size: 1012 4173
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5185
BstBI TT'CG_AA
Cuts at: 3989 3989
Size: 5185
BstEII G'GTnAC_C
Cuts at: 1184 2252 1184
Size: 1068 4117
BstYI r'GATC_y
Cuts at: 1972 2428 2515 2882 3267 3284 4736 4822 4834
Size: 456 87 367 385 17 1452 86 12
Cuts at: 4834 5180 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 456 385 367 346 87 86
17 12
BstZ17I GTA'TAC
Cuts at: 2264 2264
Size: 5185
BtgI C'CryG_G
Cuts at: 513 1065 2004 2432 2444 513
Size: 552 939 428 12 3254
Fragments arranged by size:
3254 939 552 428 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4446
Fragments arranged by size:
4446 683 56
BtsI GCAGTG_nn'
Cuts at: 2701 3495 3515 2701
Size: 794 20 4371
Fragments arranged by size:
4371 794 20
ClaI AT'CG_AT
Cuts at: 1554 2185 1554
Size: 631 4554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5150
DraI TTT'AAA
Cuts at: 2489 2489
Size: 5185
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4192 809
Size: 507 2876 1802
Fragments arranged by size:
2876 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2632 3521 5150
Size: 54 1425 50 275 18 802 889 1629
Cuts at: 5150 8
Size: 43
Fragments arranged by size:
1629 1425 889 802 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2632 1537
Size: 275 820 4090
Fragments arranged by size:
4090 820 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3115 1353
Size: 1762 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3772 4146 4292 387
Size: 1064 138 2183 374 146 1280
Fragments arranged by size:
2183 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3248 3845 4632 4900 1239
Size: 393 1616 597 787 268 1524
Fragments arranged by size:
1616 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2494 2872 2494
Size: 378 4807
FspI TGC'GCA
Cuts at: 3691 3691
Size: 5185
HaeII r_GCGC'y
Cuts at: 1678 4332 1678
Size: 2654 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3826 3858 3900 3932 1373
Size: 32 135 32 2254 32 42 32 2626
Fragments arranged by size:
2626 2254 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2529 2775 669
Size: 988 281 591 246 3079
Fragments arranged by size:
3079 988 591 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5185
HpaI GTT'AAC
Cuts at: 2775 2775
Size: 5185
KpnI G_GTAC'C
Cuts at: 2510 2510
Size: 5185
MfeI C'AATT_G
Cuts at: 2293 2784 2293
Size: 491 4694
MluI A'CGCG_T
Cuts at: 5061 5061
Size: 5185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4299 4483 1305
Size: 2994 184 2007
Fragments arranged by size:
2994 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3363
Fragments arranged by size:
3363 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3143 3502 3661 518
Size: 648 996 981 359 159 2042
Fragments arranged by size:
2042 996 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2601 3278 4426 4671 1606
Size: 995 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3694
Fragments arranged by size:
3694 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5185
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5185
NotI GC'GGCC_GC
Cuts at: 2632 2632
Size: 5185
NspI r_CATG'y
Cuts at: 1132 1229 2418 2543 4088 1132
Size: 97 1189 125 1545 2229
Fragments arranged by size:
2229 1545 1189 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5185
PciI A'CATG_T
Cuts at: 4084 4084
Size: 5185
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5185
PmeI GTTT'AAAC
Cuts at: 2489 2489
Size: 5185
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 4767 4799 3254
Size: 32 1481 32 3640
Fragments arranged by size:
3640 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5185
PsiI TTA'TAA
Cuts at: 2755 2755
Size: 5185
PstI C_TGCA'G
Cuts at: 830 2375 2537 830
Size: 1545 162 3478
Fragments arranged by size:
3478 1545 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5185
SalI G'TCGA_C
Cuts at: 1936 2527 1936
Size: 591 4594
SbfI CC_TGCA'GG
Cuts at: 2537 2537
Size: 5185
ScaI AGT'ACT
Cuts at: 3433 5145 3433
Size: 1712 3473
SfcI C'TryA_G
Cuts at: 826 1045 2371 2533 3668 4349 4540 826
Size: 219 1326 162 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 219 191 162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5185
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5185
SmaI CCC'GGG
Cuts at: 1872 2512 1872
Size: 640 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3289 4190 4452 820
Size: 197 926 1346 901 262 1553
Fragments arranged by size:
1553 1346 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2976 493
Size: 2483 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5185
SphI G_CATG'C
Cuts at: 1132 2543 1132
Size: 1411 3774
SspI AAT'ATT
Cuts at: 5 52 2362 3109 5
Size: 47 2310 747 2081
Fragments arranged by size:
2310 2081 747 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5185
StyI C'CwwG_G
Cuts at: 513 1065 2004 2624 513
Size: 552 939 620 3074
Fragments arranged by size:
3074 939 620 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 3212 3229 3382 3567 4012 325
Size: 1255 471 1161 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1161 471 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3431 5143 96
Size: 274 80 33 51 1443 1454 1712 138
Fragments arranged by size:
1712 1454 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5145 5177 5145
Size: 32 5153
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5185
XbaI T'CTAG_A
Cuts at: 2521 2521
Size: 5185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5185
XmaI C'CCGG_G
Cuts at: 1870 2510 1870
Size: 640 4545
XmnI GAAnn'nnTTC
Cuts at: 3314 3314
Size: 5185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2993 276
Size: 53 83 186 936 1459 2468
Fragments arranged by size:
2468 1459 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI