(Linear) (Six-base) MAPSORT of: KIEE2085.seq Check: 1742 from: 1 to: 2277 KIEE2085.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 663 2277 Size: 663 1614 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1633 1684 1806 2277 Size: 1633 51 122 471 Fragments arranged by size: 1633 471 122 51 AflIII A'CryG_T Cuts at: 0 182 407 1908 2277 Size: 182 225 1501 369 Fragments arranged by size: 1501 369 225 182 AhdI GACnn_n'nnGTC Cuts at: 0 2129 2277 Size: 2129 148 AleI CACnn'nnGTG Cuts at: 0 478 2277 Size: 478 1799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 496 1897 2277 Size: 496 1401 380 Fragments arranged by size: 1401 496 380 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1465 1497 2277 Size: 1465 32 780 Fragments arranged by size: 1465 780 32 AlwNI CAG_nnn'CTG Cuts at: 0 1005 1788 1890 2277 Size: 1005 783 102 387 Fragments arranged by size: 1005 783 387 102 ApaLI G'TGCA_C Cuts at: 0 1621 2277 Size: 1621 656 ApoI r'AATT_y Cuts at: 0 700 1762 2012 2170 2277 Size: 700 1062 250 158 107 Fragments arranged by size: 1062 700 250 158 107 AvaI C'yCGr_G Cuts at: 0 247 2277 Size: 247 2030 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 949 982 2277 Size: 949 33 1295 Fragments arranged by size: 1295 949 33 BanII G_rGCy'C Cuts at: 0 257 920 2277 Size: 257 663 1357 Fragments arranged by size: 1357 663 257 BbsI GAAGACnn'nnnn_ Cuts at: 0 338 374 2277 Size: 338 36 1903 Fragments arranged by size: 1903 338 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1672 1706 2277 Size: 1672 34 571 Fragments arranged by size: 1672 571 34 BclI T'GATC_A Cuts at: 0 437 443 2277 Size: 437 6 1834 Fragments arranged by size: 1834 437 6 BglI GCCn_nnn'nGGC Cuts at: 0 653 2277 Size: 653 1624 Bme1580I G_kGCm'C Cuts at: 0 1625 2277 Size: 1625 652 BmgBI CAC'GTC Cuts at: 0 339 2277 Size: 339 1938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2141 2277 Size: 2141 136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1038 1306 2207 2277 Size: 1038 268 901 70 Fragments arranged by size: 1038 901 268 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1420 2277 Size: 1420 857 BsaI GGTCTCn'nnnn_ Cuts at: 0 1194 2277 Size: 1194 1083 BsaAI yAC'GTr Cuts at: 0 185 410 1122 1909 2271 2277 Size: 185 225 712 787 362 6 Fragments arranged by size: 787 712 362 225 185 6 BsaBI GATnn'nnATC Cuts at: 0 442 1054 1309 2277 Size: 442 612 255 968 Fragments arranged by size: 968 612 442 255 BsaWI w'CCGG_w Cuts at: 0 629 2277 Size: 629 1648 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 277 307 803 833 1279 1309 1778 1808 Size: 277 30 496 30 446 30 469 30 Cuts at: 1808 2277 Size: 469 Fragments arranged by size: 496 469 469 446 277 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 265 624 1294 1786 1846 2277 Size: 265 359 670 492 60 431 Fragments arranged by size: 670 492 431 359 265 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 238 1472 2277 Size: 181 57 1234 805 Fragments arranged by size: 1234 805 181 57 BsiEI CG_ry'CG Cuts at: 0 1052 2277 Size: 1052 1225 BsiHKAI G_wGCw'C Cuts at: 0 257 1625 1796 2277 Size: 257 1368 171 481 Fragments arranged by size: 1368 481 257 171 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1177 2277 Size: 1177 1100 Bsp1286I G_dGCh'C Cuts at: 0 257 920 1625 1796 2277 Size: 257 663 705 171 481 Fragments arranged by size: 705 663 481 257 171 BspHI T'CATG_A Cuts at: 0 440 1307 2277 Size: 440 867 970 Fragments arranged by size: 970 867 440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 663 677 2277 Size: 663 14 1600 Fragments arranged by size: 1600 663 14 BsrBI CCG'CTC Cuts at: 0 614 1152 1290 2277 Size: 614 538 138 987 Fragments arranged by size: 987 614 538 138 BssSI C'ACGA_G Cuts at: 0 40 199 2277 Size: 40 159 2078 Fragments arranged by size: 2078 159 40 BstAPI GCAn_nnn'nTGC Cuts at: 0 1005 1533 2277 Size: 1005 528 744 Fragments arranged by size: 1005 744 528 BstBI TT'CG_AA Cuts at: 0 1501 2277 Size: 1501 776 BstXI CCAn_nnnn'nTGG Cuts at: 0 126 1470 2277 Size: 126 1344 807 Fragments arranged by size: 1344 807 126 BstYI r'GATC_y Cuts at: 0 350 2277 Size: 350 1927 BtgI C'CryG_G Cuts at: 0 16 71 623 1329 1613 1847 2277 Size: 16 55 552 706 284 234 430 Fragments arranged by size: 706 552 430 284 234 55 16 BtsI GCAGTG_nn' Cuts at: 0 423 2277 Size: 423 1854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1217 1252 2277 Size: 1217 35 1025 Fragments arranged by size: 1217 1025 35 DraI TTT'AAA Cuts at: 0 395 1733 1746 2277 Size: 395 1338 13 531 Fragments arranged by size: 1338 531 395 13 DraIII CAC_nnn'GTG Cuts at: 0 2271 2277 Size: 2271 6 EaeI y'GGCC_r Cuts at: 0 80 1597 1844 2277 Size: 80 1517 247 433 Fragments arranged by size: 1517 433 247 80 EarI CTCTTCn'nnn_ Cuts at: 0 704 800 1783 2277 Size: 704 96 983 494 Fragments arranged by size: 983 704 494 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 204 2277 Size: 19 185 2073 Fragments arranged by size: 2073 185 19 EcoICRI GAG'CTC Cuts at: 0 255 2277 Size: 255 2022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1038 1306 1633 1684 1806 2207 2277 Size: 1038 268 327 51 122 401 70 Fragments arranged by size: 1038 401 327 268 122 70 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 234 1168 2277 Size: 234 934 1109 Fragments arranged by size: 1109 934 234 EcoO109I rG'GnC_Cy Cuts at: 0 12 1555 2277 Size: 12 1543 722 Fragments arranged by size: 1543 722 12 EcoRI G'AATT_C Cuts at: 0 1762 2277 Size: 1762 515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 45 1194 2277 Size: 45 1149 1083 Fragments arranged by size: 1149 1083 45 FspI TGC'GCA Cuts at: 0 1873 2277 Size: 1873 404 HaeII r_GCGC'y Cuts at: 0 40 1006 2277 Size: 40 966 1271 Fragments arranged by size: 1271 966 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 422 454 1908 1940 2115 2147 2277 Size: 422 32 1454 32 175 32 130 Fragments arranged by size: 1454 422 175 130 32 32 32 HincII GTy'rAC Cuts at: 0 1161 2277 Size: 1161 1116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1076 1277 2277 Size: 1076 201 1000 Fragments arranged by size: 1076 1000 201 MscI TGG'CCA Cuts at: 0 1599 1846 2277 Size: 1599 247 431 Fragments arranged by size: 1599 431 247 MslI CAynn'nnrTG Cuts at: 0 478 1612 1618 2277 Size: 478 1134 6 659 Fragments arranged by size: 1134 659 478 6 MspA1I CmG'CkG Cuts at: 0 18 73 972 2166 2277 Size: 18 55 899 1194 111 Fragments arranged by size: 1194 899 111 55 18 NcoI C'CATG_G Cuts at: 0 1613 2277 Size: 1613 664 NdeI CA'TA_TG Cuts at: 0 1532 2277 Size: 1532 745 NspI r_CATG'y Cuts at: 0 660 1237 1482 1726 2277 Size: 660 577 245 244 551 Fragments arranged by size: 660 577 551 245 244 PflMI CCAn_nnn'nTGG Cuts at: 0 267 930 1788 2271 2277 Size: 267 663 858 483 6 Fragments arranged by size: 858 663 483 267 6 PmlI CAC'GTG Cuts at: 0 185 410 1122 1909 2277 Size: 185 225 712 787 368 Fragments arranged by size: 787 712 368 225 185 PsiI TTA'TAA Cuts at: 0 1358 2277 Size: 1358 919 PspXI vC'TCGA_Gb Cuts at: 0 247 2277 Size: 247 2030 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 958 990 2277 Size: 958 32 1287 Fragments arranged by size: 1287 958 32 PstI C_TGCA'G Cuts at: 0 430 488 674 911 1013 1218 1886 2277 Size: 430 58 186 237 102 205 668 391 Fragments arranged by size: 668 430 391 237 205 186 102 58 PvuI CG_AT'CG Cuts at: 0 1052 2277 Size: 1052 1225 PvuII CAG'CTG Cuts at: 0 972 2166 2277 Size: 972 1194 111 Fragments arranged by size: 1194 972 111 SacI G_AGCT'C Cuts at: 0 257 2277 Size: 257 2020 SacII CC_GC'GG Cuts at: 0 19 74 2277 Size: 19 55 2203 Fragments arranged by size: 2203 55 19 SbfI CC_TGCA'GG Cuts at: 0 674 2277 Size: 674 1603 SfcI C'TryA_G Cuts at: 0 426 484 670 907 1009 1214 1559 1882 Size: 426 58 186 237 102 205 345 323 Cuts at: 1882 2277 Size: 395 Fragments arranged by size: 426 395 345 323 237 205 186 102 58 SmlI C'TyrA_G Cuts at: 0 247 1435 2277 Size: 247 1188 842 Fragments arranged by size: 1188 842 247 SphI G_CATG'C Cuts at: 0 660 2277 Size: 660 1617 StuI AGG'CCT Cuts at: 0 325 2277 Size: 325 1952 StyI C'CwwG_G Cuts at: 0 1613 2277 Size: 1613 664 TatI w'GTAC_w Cuts at: 0 1025 2277 Size: 1025 1252 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 277 309 1778 1810 1925 1957 2277 Size: 277 32 1469 32 115 32 320 Fragments arranged by size: 1469 320 277 115 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 337 1081 2277 Size: 337 744 1196 Fragments arranged by size: 1196 744 337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 513 2277 Size: 513 1764 XhoI C'TCGA_G Cuts at: 0 247 2277 Size: 247 2030 XmnI GAAnn'nnTTC Cuts at: 0 1762 2277 Size: 1762 515 Enzymes that do cut: AarI AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AvaI BaeI BanII BbsI BcgI BclI BglI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstBI BstXI BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI NspI PflMI PmlI PsiI PspXI PsrI PstI PvuI PvuII SacI SacII SbfI SfcI SmlI SphI StuI StyI TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI ApaI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BciVI BfrBI BglII BlpI BmrI BmtI Bpu10I BsaHI BseYI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BstEII BstZ17I Bsu36I BtgZI ClaI DrdI EagI EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI RsrII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII XbaI XmaI ZraI