################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HhaI HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcyI 684 GACGCC G[GA]^CG[CT]C AflII 184 CTTAAG C^TTAAG AflIII 348 ACGTGT A^C[GA][CT]GT AgeI 9 ACCGGT A^CCGGT Asp700I 132 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 281 GAGCTC G[AT]GC[AT]^C AvaII 666 GGTCC G^G[AT]CC BanI 12 GGTGCC G^G[CT][GA]CC BanII 281 GAGCTC G[GA]GC[CT]^C BfrI 184 CTTAAG C^TTAAG BsaAI 347 TACGTG [CT]AC^GT[GA] BsaHI 684 GACGCC G[GA]^CG[CT]C BsaWI 9 ACCGGT [AT]^CCGG[AT] BsiHKAI 281 GAGCTC G[AT]GC[AT]^C BsmI 439 GAATGC GAATGC(1/-1) Bsp1407I 497 TGTACA T^GTACA BsrFI 9 ACCGGT [GA]^CCGG[CT] BsrGI 497 TGTACA T^GTACA BstAPI 153 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 406 GGTTACC G^GT[ACGT]ACC BstPI 406 GGTTACC G^GT[ACGT]ACC BstXI 608 CCAAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 63 GGATCT [GA]^GATC[CT] Cfr10I 9 ACCGGT [GA]^CCGG[CT] Cfr13I 666 GGTCC G^G[ACGT]CC DraI 448 TTTAAA TTT^AAA EcoO65I 406 GGTTACC G^GT[ACGT]ACC EcoT14I 296 CCAAGG C^C[AT][AT]GG HapII 10 CCGG C^CGG Hin1I 684 GACGCC G[GA]^CG[CT]C HincII 736 GTCAAC GT[CT]^[GA]AC HindII 736 GTCAAC GT[CT]^[GA]AC HpaII 10 CCGG C^CGG HphI 681 GGTGA GGTGA(8/7) MaeII 348 ACGT A^CGT MflI 63 GGATCT [GA]^GATC[CT] MspI 10 CCGG C^CGG NdeI 154 CATATG CA^TATG PinAI 9 ACCGGT A^CCGGT PleI 180 GAGTC GAGTC(4/5) PstI 160 CTGCAG CTGCA^G SacI 281 GAGCTC GAGCT^C Sau96I 666 GGTCC G^G[ACGT]CC SexAI 232 ACCAGGT A^CC[AT]GGT SfcI 160 CTGCAG C^T[GA][CT]AG SspBI 497 TGTACA T^GTACA StyI 296 CCAAGG C^C[AT][AT]GG TaqI 287 TCGA T^CGA TthHB8I 287 TCGA T^CGA XhoII 63 GGATCT [GA]^GATC[CT] XmnI 132 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 498 GTAC GT^AC 630 GTAC AlwI 208 GATCC GGATC(4/5) 63 GGATC BmyI 13 GTGCCC G[AGT]GC[ACT]^C 281 GAGCTC BsmFI 342 GGGAC GGGAC(10/14) 667 GTCCC Bsp1286I 13 GTGCCC G[AGT]GC[ACT]^C 281 GAGCTC BsrI 337 CCAGT ACTGG(1/-1) 733 CCAGT Cac8I 245 GCAGGC GC[ACGT]^[ACGT]GC 552 GCAAGC DdeI 76 CTCAG C^T[ACGT]AG 86 CTAAG DpnII 64 GATC ^GATC 208 GATC HgaI 684 GACGC GACGC(5/10) 706 GCGTC MboI 64 GATC ^GATC 208 GATC MslI 271 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 333 CATACCAGTG MwoI 153 GCATATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 245 GCAGGCAAGGC NdeII 64 GATC ^GATC 208 GATC RsaI 498 GTAC GT^AC 630 GTAC Sau3AI 64 GATC ^GATC 208 GATC SmlI 184 CTTAAG C^T[CT][GA]AG 217 CTCAAG TfiI 454 GAATC G^A[AT]TC 511 GAATC Tsp45I 229 GTGAC ^GT[GC]AC 682 GTGAC TspRI 163 CAGTG CA[GC]TG[ACGT][ACGT]^ 338 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI EarI EcoRII Fnu4HI FokI HinfI ItaI Ksp632I MaeIII MboII MnlI MseI MvaI NlaIII NlaIV ScrFI Tru9I TseI Tsp509I