(Circular) (Six-base) MAPSORT of: pFN21AE2244.seq Check: 7332 from: 1 to: 5461 pFN21AE2244 5461 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2804 1937 Size: 867 4594 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2011 3524 4908 5176 1632 Size: 379 1513 1384 268 1917 Fragments arranged by size: 1917 1513 1384 379 268 AflII C'TTAA_G Cuts at: 820 1017 2189 820 Size: 197 1172 4092 Fragments arranged by size: 4092 1172 197 AflIII A'CryG_T Cuts at: 1203 2353 4360 5337 1203 Size: 1150 2007 977 1327 Fragments arranged by size: 2007 1327 1150 977 AgeI A'CCGG_T Cuts at: 2014 4292 2014 Size: 2278 3183 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5461 AlwNI CAG_nnn'CTG Cuts at: 3204 4776 3204 Size: 1572 3889 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2786 1870 Size: 73 843 4545 Fragments arranged by size: 4545 843 73 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2782 4237 618 Size: 325 217 627 230 765 1455 1842 Fragments arranged by size: 1842 1455 765 627 325 230 217 BanII G_rGCy'C Cuts at: 1391 2290 2780 1391 Size: 899 490 4072 Fragments arranged by size: 4072 899 490 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2022 3522 4678 1790 Size: 232 1500 1156 2573 Fragments arranged by size: 2573 1500 1156 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 4150 442 Size: 416 53 83 399 24 1775 958 1753 Fragments arranged by size: 1775 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4121 1239 Size: 2882 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 3586 4451 4749 2207 Size: 1379 865 298 2919 Fragments arranged by size: 2919 1379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 882 Size: 617 2625 2219 Fragments arranged by size: 2625 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2354 3252 493 Size: 711 1150 898 2702 Fragments arranged by size: 2702 1150 898 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2690 3268 3650 4285 Size: 53 83 186 936 1157 578 382 635 Cuts at: 4285 275 Size: 1451 Fragments arranged by size: 1451 1157 936 635 578 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2014 3894 4292 4566 4713 5124 2014 Size: 1880 398 274 147 411 2351 Fragments arranged by size: 2351 1880 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 2217 4664 5160 2217 Size: 2447 496 2518 Fragments arranged by size: 2518 2447 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4888 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3672 4700 1540 Size: 275 186 910 761 1028 2301 Fragments arranged by size: 2301 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2290 3522 3607 4678 2290 Size: 1232 85 1071 3073 Fragments arranged by size: 3073 1232 1071 85 BsmI GAATG_Cn' Cuts at: 2450 2972 3065 2450 Size: 522 93 4846 Fragments arranged by size: 4846 522 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2290 2780 3522 3607 4678 1391 Size: 399 232 268 490 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 268 232 85 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 844 Size: 1972 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 4105 4292 1700 Size: 110 204 2091 187 2869 Fragments arranged by size: 2869 2091 204 187 110 BsrGI T'GTAC_A Cuts at: 96 2502 96 Size: 2406 3055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 2164 794 Size: 1370 4091 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 2411 1184 Size: 1227 4234 BstXI CCAn_nnnn'nTGG Cuts at: 2620 2620 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2068 2791 3158 3543 3560 5012 5098 5110 Size: 96 723 367 385 17 1452 86 12 Cuts at: 5110 5456 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 723 385 367 346 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2174 2977 3771 3791 2174 Size: 803 794 20 3844 Fragments arranged by size: 3844 803 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2455 2765 2455 Size: 310 5151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426 Size: 54 1425 50 275 18 1078 889 1629 Cuts at: 5426 8 Size: 43 Fragments arranged by size: 1629 1425 1078 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2078 2333 3391 1353 Size: 677 48 255 1058 3423 Fragments arranged by size: 3423 1058 677 255 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 4422 4568 387 Size: 1064 138 2459 374 146 1280 Fragments arranged by size: 2459 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2288 2288 Size: 5461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 3524 4121 4908 5176 1239 Size: 393 379 1513 597 787 268 1524 Fragments arranged by size: 1524 1513 787 597 393 379 268 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 5461 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 4608 1678 Size: 2930 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373 Size: 32 135 32 2530 32 42 32 2626 Fragments arranged by size: 2626 2530 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2743 2805 3051 669 Size: 988 281 805 62 246 3079 Fragments arranged by size: 3079 988 805 281 246 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4575 4759 1305 Size: 3270 184 2007 Fragments arranged by size: 3270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3639 Fragments arranged by size: 3639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 2342 3419 3778 3937 518 Size: 648 1114 62 1077 359 159 2042 Fragments arranged by size: 2042 1114 1077 648 359 159 62 MspA1I CmG'CkG Cuts at: 1606 2877 3554 4702 4947 1606 Size: 1271 677 1148 245 2120 Fragments arranged by size: 2120 1271 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 NdeI CA'TA_TG Cuts at: 387 2160 387 Size: 1773 3688 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2819 4364 1132 Size: 97 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PstI C_TGCA'G Cuts at: 830 2169 2813 830 Size: 1339 644 3478 Fragments arranged by size: 3478 1339 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SacI G_AGCT'C Cuts at: 2290 2290 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SexAI A'CCwGG_T Cuts at: 2237 2237 Size: 5461 SfcI C'TryA_G Cuts at: 826 1045 2165 2809 3944 4625 4816 826 Size: 219 1120 644 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 681 644 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 2222 3565 4466 4728 820 Size: 197 926 246 33 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 262 246 197 33 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5461 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2900 513 Size: 552 939 297 599 3074 Fragments arranged by size: 3074 939 599 552 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 3707 5419 Size: 274 80 33 51 1443 525 1205 1712 Cuts at: 5419 96 Size: 138 Fragments arranged by size: 1712 1443 1205 525 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 2141 3590 2141 Size: 1449 4012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI