(Linear) (Six-base) MAPSORT of: KIEE5751.seq Check: 8545 from: 1 to: 2124 KIEE5751.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1125 2124 Size: 1125 999 AccI GT'mk_AC Cuts at: 0 269 2124 Size: 269 1855 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1718 1837 2124 Size: 1718 119 287 Fragments arranged by size: 1718 287 119 AhdI GACnn_n'nnGTC Cuts at: 0 286 2124 Size: 286 1838 AlwNI CAG_nnn'CTG Cuts at: 0 1342 2124 Size: 1342 782 ApaI G_GGCC'C Cuts at: 0 1398 2124 Size: 1398 726 ApaLI G'TGCA_C Cuts at: 0 358 2124 Size: 358 1766 ApoI r'AATT_y Cuts at: 0 126 569 956 1645 1810 2124 Size: 126 443 387 689 165 314 Fragments arranged by size: 689 443 387 314 165 126 AseI AT'TA_AT Cuts at: 0 308 509 2124 Size: 308 201 1615 Fragments arranged by size: 1615 308 201 AvaI C'yCGr_G Cuts at: 0 400 2124 Size: 400 1724 BanI G'GyrC_C Cuts at: 0 337 2124 Size: 337 1787 BanII G_rGCy'C Cuts at: 0 213 904 1398 1490 2124 Size: 213 691 494 92 634 Fragments arranged by size: 691 634 494 213 92 BbsI GAAGACnn'nnnn_ Cuts at: 0 1155 1782 2124 Size: 1155 627 342 Fragments arranged by size: 1155 627 342 BclI T'GATC_A Cuts at: 0 1001 2124 Size: 1001 1123 BfrBI ATG'CAT Cuts at: 0 1276 2124 Size: 1276 848 BglI GCCn_nnn'nGGC Cuts at: 0 1108 1996 2124 Size: 1108 888 128 Fragments arranged by size: 1108 888 128 Bme1580I G_kGCm'C Cuts at: 0 362 1398 2124 Size: 362 1036 726 Fragments arranged by size: 1036 726 362 BmrI ACTGGGnnnn_n' Cuts at: 0 798 2124 Size: 798 1326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 888 1256 2124 Size: 428 460 368 868 Fragments arranged by size: 868 460 428 368 Bpu10I CC'TnA_GC Cuts at: 0 40 2124 Size: 40 2084 BsaAI yAC'GTr Cuts at: 0 363 2124 Size: 363 1761 BsaBI GATnn'nnATC Cuts at: 0 1000 2124 Size: 1000 1124 BsaHI Gr'CG_yC Cuts at: 0 196 2124 Size: 196 1928 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1229 1259 2124 Size: 1229 30 865 Fragments arranged by size: 1229 865 30 BseYI C'CCAG_C Cuts at: 0 137 231 2022 2124 Size: 137 94 1791 102 Fragments arranged by size: 1791 137 102 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1153 2124 Size: 1153 971 BsiHKAI G_wGCw'C Cuts at: 0 213 362 779 1490 1901 2124 Size: 213 149 417 711 411 223 Fragments arranged by size: 711 417 411 223 213 149 BsmI GAATG_Cn' Cuts at: 0 101 2124 Size: 101 2023 Bsp1286I G_dGCh'C Cuts at: 0 213 362 779 904 1398 1490 1901 2124 Size: 213 149 417 125 494 92 411 223 Fragments arranged by size: 494 417 411 223 213 149 125 92 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1125 2124 Size: 1125 999 BsrBI CCG'CTC Cuts at: 0 813 2124 Size: 813 1311 BsrDI GCAATG_nn' Cuts at: 0 1040 2124 Size: 1040 1084 BsrGI T'GTAC_A Cuts at: 0 665 2124 Size: 665 1459 BssSI C'ACGA_G Cuts at: 0 225 2124 Size: 225 1899 BstAPI GCAn_nnn'nTGC Cuts at: 0 366 2124 Size: 366 1758 BstXI CCAn_nnnn'nTGG Cuts at: 0 878 2124 Size: 878 1246 BtgI C'CryG_G Cuts at: 0 1106 1874 2124 Size: 1106 768 250 Fragments arranged by size: 1106 768 250 BtsI GCAGTG_nn' Cuts at: 0 663 913 1340 2124 Size: 663 250 427 784 Fragments arranged by size: 784 663 427 250 ClaI AT'CG_AT Cuts at: 0 1145 2124 Size: 1145 979 DraIII CAC_nnn'GTG Cuts at: 0 366 2124 Size: 366 1758 DrdI GACnn_nn'nnGTC Cuts at: 0 252 2124 Size: 252 1872 EaeI y'GGCC_r Cuts at: 0 707 2124 Size: 707 1417 EarI CTCTTCn'nnn_ Cuts at: 0 449 1160 1235 2124 Size: 449 711 75 889 Fragments arranged by size: 889 711 449 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1595 2124 Size: 1595 529 EcoICRI GAG'CTC Cuts at: 0 211 1488 2124 Size: 211 1277 636 Fragments arranged by size: 1277 636 211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 888 1256 1718 1837 2124 Size: 428 460 368 462 119 287 Fragments arranged by size: 462 460 428 368 287 119 EcoO109I rG'GnC_Cy Cuts at: 0 1395 2124 Size: 1395 729 EcoRI G'AATT_C Cuts at: 0 569 1810 2124 Size: 569 1241 314 Fragments arranged by size: 1241 569 314 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 136 168 200 232 272 304 1322 1354 Size: 136 32 32 32 40 32 1018 32 Cuts at: 1354 2022 2054 2124 Size: 668 32 70 Fragments arranged by size: 1018 668 136 70 40 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1618 1737 2124 Size: 1618 119 387 Fragments arranged by size: 1618 387 119 HindIII A'AGCT_T Cuts at: 0 584 2124 Size: 584 1540 HpaI GTT'AAC Cuts at: 0 1737 2124 Size: 1737 387 MfeI C'AATT_G Cuts at: 0 275 2124 Size: 275 1849 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1859 2124 Size: 1859 265 MscI TGG'CCA Cuts at: 0 709 2124 Size: 709 1415 MslI CAynn'nnrTG Cuts at: 0 329 544 1501 1894 2124 Size: 329 215 957 393 230 Fragments arranged by size: 957 393 329 230 215 MspA1I CmG'CkG Cuts at: 0 137 1691 2026 2124 Size: 137 1554 335 98 Fragments arranged by size: 1554 335 137 98 NcoI C'CATG_G Cuts at: 0 1106 2124 Size: 1106 1018 NsiI A_TGCA'T Cuts at: 0 1278 2124 Size: 1278 846 PfoI T'CCnGG_A Cuts at: 0 934 1233 1326 2124 Size: 934 299 93 798 Fragments arranged by size: 934 798 299 93 PmlI CAC'GTG Cuts at: 0 363 2124 Size: 363 1761 PspOMI G'GGCC_C Cuts at: 0 1394 2124 Size: 1394 730 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 658 690 2124 Size: 658 32 1434 Fragments arranged by size: 1434 658 32 PstI C_TGCA'G Cuts at: 0 920 2124 Size: 920 1204 PvuII CAG'CTG Cuts at: 0 137 1691 2124 Size: 137 1554 433 Fragments arranged by size: 1554 433 137 SacI G_AGCT'C Cuts at: 0 213 1490 2124 Size: 213 1277 634 Fragments arranged by size: 1277 634 213 SexAI A'CCwGG_T Cuts at: 0 1409 2124 Size: 1409 715 SfcI C'TryA_G Cuts at: 0 629 916 2124 Size: 629 287 1208 Fragments arranged by size: 1208 629 287 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1108 2124 Size: 1108 1016 StyI C'CwwG_G Cuts at: 0 651 1106 2124 Size: 651 455 1018 Fragments arranged by size: 1018 651 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 558 2124 Size: 558 1566 TatI w'GTAC_w Cuts at: 0 116 665 1943 2124 Size: 116 549 1278 181 Fragments arranged by size: 1278 549 181 116 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1229 1261 2124 Size: 1229 32 863 Fragments arranged by size: 1229 863 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 331 498 1113 1134 2124 Size: 331 167 615 21 990 Fragments arranged by size: 990 615 331 167 21 Enzymes that do cut: AarI AccI AcuI AhdI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbsI BclI BfrBI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI PfoI PmlI PspOMI PsrI PstI PvuII SacI SexAI SfcI SfiI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI CspCI DraI EagI EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI