################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbeI BbrPI BbvCI BcgI BclI BfaI BglI BlnI BsaAI BsaBI BsaHI BseAI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 637 CGCG CG^CG AflII 168 CTTAAG C^TTAAG AspHI 331 GAGCTC G[AT]GC[AT]^C AvaI 1339 CCCGAG C^[CT]CG[GA]G AviII 281 TGCGCA TGC^GCA BalI 1602 TGGCCA TGG^CCA BamHI 1560 GGATCC G^GATCC BanI 112 GGTGCC G^G[CT][GA]CC BciVI 731 GTATCC GTATCC(6/5) BfrI 168 CTTAAG C^TTAAG BglII 1051 AGATCT A^GATCT BlpI 1061 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1061 GCTCAGC GC^T[ACGT]AGC BsaI 1622 GGTCTC GGTCTC(1/5) BsaWI 1568 ACCGGA [AT]^CCGG[AT] BseRI 1255 GAGGAG GAGGAG(10/8) BsiEI 1566 CGACCG CG[GA][CT]^CG BsiHKAI 331 GAGCTC G[AT]GC[AT]^C BsiWI 107 CGTACG C^GTACG BsmBI 602 GAGACG CGTCTC(1/5) BsmFI 1517 GTCCC GGGAC(10/14) BsoBI 1339 CCCGAG C^[CT]CG[GA]G Bsp1407I 392 TGTACA T^GTACA BsrBI 1059 CCGCTC CCGCTC(-3/-3) BsrFI 1145 ACCGGC [GA]^CCGG[CT] BsrGI 392 TGTACA T^GTACA BstAPI 1493 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 630 GGTTACC G^GT[ACGT]ACC BstPI 630 GGTTACC G^GT[ACGT]ACC BstUI 637 CGCG CG^CG BstXI 203 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1061 GCTCAGC GC^T[ACGT]AGC Cfr10I 1145 ACCGGC [GA]^CCGG[CT] DraIII 855 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1144 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1520 CCACGG C^C[GA][CT]GG EaeI 1602 TGGCCA [CT]^GGCC[GA] EcoO65I 630 GGTTACC G^GT[ACGT]ACC EcoRI 402 GAATTC G^AATTC FspI 281 TGCGCA TGC^GCA HindIII 1137 AAGCTT A^AGCTT MluNI 1602 TGGCCA TGG^CCA MscI 1602 TGGCCA TGG^CCA MvnI 637 CGCG CG^CG PvuII 520 CAGCTG CAG^CTG SacI 331 GAGCTC GAGCT^C SspBI 392 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 1084 ACACGT A^C[GA][CT]GT 1511 ACGTGT AocI 181 CCTCAGG CC^T[ACGT]AGG 1105 CCTGAGG BcnI 62 CCGGG CC^[GC]GG 611 CCCGG BsgI 198 CTGCAC GTGCAG(16/14) 1659 GTGCAG BsmI 342 GAATGC GAATGC(1/-1) 1261 GAATGC BstYI 1051 AGATCT [GA]^GATC[CT] 1560 GGATCC Bsu36I 181 CCTCAGG CC^T[ACGT]AGG 1105 CCTGAGG CfoI 282 GCGC GCG^C 638 GCGC Eco81I 181 CCTCAGG CC^T[ACGT]AGG 1105 CCTGAGG EcoT14I 116 CCAAGG C^C[AT][AT]GG 707 CCAAGG HhaI 282 GCGC GCG^C 638 GCGC HinP1I 282 GCGC G^CGC 638 GCGC MflI 1051 AGATCT [GA]^GATC[CT] 1560 GGATCC MspA1I 520 CAGCTG C[AC]G^C[GT]G 1238 CAGCGG NciI 62 CCGGG CC^[GC]GG 611 CCCGG PleI 1582 GACTC GAGTC(4/5) 925 GAGTC PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT] 655 AGGACCT PshAI 657 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1510 GACGTGTGTC SfaNI 750 GATGC GCATC(5/9) 877 GATGC SmlI 168 CTTAAG C^T[CT][GA]AG 589 CTCAAG SphI 386 GCATGC GCATG^C 989 GCATGC StyI 116 CCAAGG C^C[AT][AT]GG 707 CCAAGG TaqI 242 TCGA T^CGA 851 TCGA TthHB8I 242 TCGA T^CGA 851 TCGA XhoII 1051 AGATCT [GA]^GATC[CT] 1560 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI