################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MaeI MluI MroI MspA1I MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 714 GCGG CCGC(-3/-1) AclI 220 AACGTT AA^CGTT AfaI 366 GTAC GT^AC AflIII 362 ACATGT A^C[GA][CT]GT Asp700I 219 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 239 TGGCCA TGG^CCA BanI 61 GGCACC G^G[CT][GA]CC BglII 249 AGATCT A^GATCT BlpI 586 GCTAAGC GC^T[ACGT]AGC Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC BsaBI 489 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 320 GAGACC GGTCTC(1/5) BsmAI 320 GAGAC GTCTC(1/5) BsmFI 697 GTCCC GGGAC(10/14) Bsp1407I 365 TGTACA T^GTACA BspLU11I 362 ACATGT A^CATGT BsrDI 415 GCAATG GCAATG(2/0) BsrGI 365 TGTACA T^GTACA BsrI 870 ACTGG ACTGG(1/-1) CelII 586 GCTAAGC GC^T[ACGT]AGC DraII 695 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 239 TGGCCA [CT]^GGCC[GA] EcoO109I 695 AGGTCCC [GA]G^G[ACGT]CC[CT] FauI 714 GCGGG CCCGC(4/6) HincII 358 GTCAAC GT[CT]^[GA]AC HindII 358 GTCAAC GT[CT]^[GA]AC HindIII 854 AAGCTT A^AGCTT MaeII 221 ACGT A^CGT MamI 489 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 239 TGGCCA TGG^CCA MscI 239 TGGCCA TGG^CCA MslI 210 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 483 CATATG CA^TATG PleI 155 GAGTC GAGTC(4/5) PpuMI 695 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 944 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 220 AACGTT AA^CGTT RsaI 366 GTAC GT^AC SbfI 329 CCTGCAGG CCTGCA^GG Sse8387I 329 CCTGCAGG CCTGCA^GG SspBI 365 TGTACA T^GTACA SspI 141 AATATT AAT^ATT XmnI 219 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 84 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 651 CAGCATCTG AvaII 696 GGTCC G^G[AT]CC 800 GGACC BamHI 467 GGATCC G^GATCC 488 GGATCC BclI 668 TGATCA T^GATCA 758 TGATCA BsaJI 614 CCTTGG C^C[ACGT][ACGT]GG 875 CCTTGG BsgI 90 CTGCAC GTGCAG(16/14) 953 CTGCAC BstAPI 83 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 883 GCAGCCAATGC BstF5I 29 CATCC GGATG(2/0) 970 CATCC Cfr13I 696 GGTCC G^G[ACGT]CC 800 GGACC EcoT14I 614 CCTTGG C^C[AT][AT]GG 875 CCTTGG FbaI 668 TGATCA T^GATCA 758 TGATCA FokI 29 CATCC GGATG(9/13) 970 CATCC MaeIII 445 GTCAC ^GT[ACGT]AC 550 GTCAC MboII 764 TCTTC GAAGA(8/7) 767 TCTTC MseI 685 TTAA T^TAA 935 TTAA NspI 362 ACATGT [GA]CATG^[CT] 441 GCATGT Sau96I 696 GGTCC G^G[ACGT]CC 800 GGACC SfaNI 452 GCATC GCATC(5/9) 653 GCATC StyI 614 CCTTGG C^C[AT][AT]GG 875 CCTTGG TfiI 570 GAATC G^A[AT]TC 703 GAATC Tru9I 685 TTAA T^TAA 935 TTAA Tsp45I 445 GTCAC ^GT[GC]AC 550 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BsiYI BslI BssKI BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MboI MfeI MflI MnlI MunI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI ScrFI SfcI TseI Tsp509I TspRI XhoII