(Circular) (Six-base) MAPSORT of: pFN21AE5530.seq Check: 7595 from: 1 to: 5995 pFN21AE5530 5995 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2270 2857 3338 1937 Size: 333 587 481 4594 Fragments arranged by size: 4594 587 481 333 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2626 2997 4058 5442 5710 1632 Size: 467 527 371 1061 1384 268 1917 Fragments arranged by size: 1917 1384 1061 527 467 371 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2015 3738 5310 2015 Size: 1723 1572 2700 Fragments arranged by size: 2700 1723 1572 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2228 2393 2481 2729 3159 3304 3530 3682 Size: 735 165 88 248 430 145 226 152 Cuts at: 3682 5791 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 735 430 248 226 165 152 145 88 AseI AT'TA_AT Cuts at: 160 1032 2345 4549 160 Size: 872 1313 2204 1606 Fragments arranged by size: 2204 1606 1313 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3320 1870 Size: 73 1377 4545 Fragments arranged by size: 4545 1377 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3314 1391 Size: 1923 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2087 2345 2994 928 Size: 971 188 258 649 3929 Fragments arranged by size: 3929 971 649 258 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 2362 1548 Size: 814 5181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3193 5990 3193 Size: 2797 3198 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 4056 5212 1790 Size: 2266 1156 2573 Fragments arranged by size: 2573 2266 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4655 1239 Size: 3416 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2451 4120 4985 5283 2451 Size: 1669 865 298 3163 Fragments arranged by size: 3163 1669 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4658 882 Size: 617 3159 2219 Fragments arranged by size: 3159 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2838 1186 Size: 1652 4343 BseYI C'CCAG_C Cuts at: 2249 5198 5694 2249 Size: 2949 496 2550 Fragments arranged by size: 2949 2550 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2868 1163 Size: 573 1132 4290 Fragments arranged by size: 4290 1132 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4056 4141 5212 4056 Size: 85 1071 4839 Fragments arranged by size: 4839 1071 85 BsmI GAATG_Cn' Cuts at: 2095 2710 2959 3506 3599 2095 Size: 615 249 547 93 4491 Fragments arranged by size: 4491 615 547 249 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3314 4056 4141 5212 1391 Size: 399 1524 742 85 1071 2174 Fragments arranged by size: 2174 1524 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 844 Size: 2506 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3882 1394 Size: 429 2059 3507 Fragments arranged by size: 3507 2059 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2209 4490 4664 65 Size: 1183 961 2281 174 1396 Fragments arranged by size: 2281 1396 1183 961 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4639 4826 1700 Size: 110 2829 187 2869 Fragments arranged by size: 2869 2829 187 110 BsrGI T'GTAC_A Cuts at: 96 3085 3206 96 Size: 2989 121 2885 Fragments arranged by size: 2989 2885 121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstXI CCAn_nnnn'nTGG Cuts at: 2674 2674 Size: 5995 BstYI r'GATC_y Cuts at: 1972 2662 2875 3193 3325 3692 4077 4094 5546 Size: 690 213 318 132 367 385 17 1452 Cuts at: 5546 5632 5644 5990 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 690 385 367 346 318 213 132 86 17 12 BstZ17I GTA'TAC Cuts at: 2271 2858 2271 Size: 587 5408 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 2434 3299 2434 Size: 865 5130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960 Size: 54 1425 50 275 18 1612 889 1629 Cuts at: 5960 8 Size: 43 Fragments arranged by size: 1629 1612 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2375 2645 3042 3925 1353 Size: 1022 270 397 883 3423 Fragments arranged by size: 3423 1022 883 397 270 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2626 2997 4058 4655 5442 5710 Size: 393 467 527 371 1061 597 787 268 Cuts at: 5710 1239 Size: 1524 Fragments arranged by size: 1524 1061 787 597 527 467 393 371 268 EcoNI CCTnn'n_nnAGG Cuts at: 3015 3015 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 3020 3020 Size: 5995 EcoRI G'AATT_C Cuts at: 2481 3159 3304 3682 2481 Size: 678 145 378 4794 Fragments arranged by size: 4794 678 378 145 EcoRV GAT'ATC Cuts at: 2607 2607 Size: 5995 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 5142 1678 Size: 3464 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 2352 748 Size: 1604 4391 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2894 5109 5293 1305 Size: 1589 2215 184 2007 Fragments arranged by size: 2215 2007 1589 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 3953 4312 4471 518 Size: 648 1591 1196 359 159 2042 Fragments arranged by size: 2042 1591 1196 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3411 4088 5236 5481 1606 Size: 1805 677 1148 245 2120 Fragments arranged by size: 2120 1805 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2895 3084 3353 4898 1132 Size: 97 1666 189 269 1545 2229 Fragments arranged by size: 2229 1666 1545 269 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5995 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3020 3020 Size: 5995 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PstI C_TGCA'G Cuts at: 830 2713 2981 3347 830 Size: 1883 268 366 3478 Fragments arranged by size: 3478 1883 366 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2709 2946 2977 3343 4478 5159 5350 Size: 219 1664 237 31 366 1135 681 191 Cuts at: 5350 826 Size: 1471 Fragments arranged by size: 1664 1471 1135 681 366 237 219 191 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2466 4099 5000 5262 820 Size: 197 926 523 1633 901 262 1553 Fragments arranged by size: 1633 1553 926 901 523 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3175 3919 5 Size: 47 3123 744 2081 Fragments arranged by size: 3123 2081 744 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2667 3041 3434 513 Size: 552 939 111 552 374 393 3074 Fragments arranged by size: 3074 939 552 552 393 374 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2921 4022 4039 4192 4377 4822 325 Size: 1255 1341 1101 17 153 185 445 1498 Fragments arranged by size: 1498 1341 1255 1101 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3001 3085 3206 Size: 274 80 33 51 1443 1024 84 121 Cuts at: 3206 4241 5953 96 Size: 1035 1712 138 Fragments arranged by size: 1712 1443 1035 1024 274 138 121 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2706 2738 5955 5987 2706 Size: 32 3217 32 2714 Fragments arranged by size: 3217 2714 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3145 3331 3145 Size: 186 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3245 3245 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI