Restriction map for
pFN21AE1803
(Circular) (Six-base) MAPSORT of: pFN21AE1803.seq Check: 9146 from: 1 to: 5656
pFN21AE1803 5656 bp
With 182 enzymes: SgfI *
May 20, 2016 17:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2565 2977 2565
Size: 412 5244
AclI AA'CG_TT
Cuts at: 1563 2832 3783 4156 1563
Size: 1269 951 373 3063
Fragments arranged by size:
3063 1269 951 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3719 5103 5371 1632
Size: 1038 1049 1384 268 1917
Fragments arranged by size:
1917 1384 1049 1038 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaI G_GGCC'C
Cuts at: 2090 2090
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2271 2803 2810 2965 3191 3343 5452 1493
Size: 778 532 7 155 226 152 2109 1697
Fragments arranged by size:
2109 1697 778 532 226 155 152 7
AseI AT'TA_AT
Cuts at: 160 1032 2336 2514 4210 160
Size: 872 1304 178 1696 1606
Fragments arranged by size:
1696 1606 1304 872 178
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2227 2902 2986 3353 2227
Size: 675 84 367 4530
Fragments arranged by size:
4530 675 367 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2565 2977 4432 618
Size: 325 217 627 778 412 1455 1842
Fragments arranged by size:
1842 1455 778 627 412 325 217
BanII G_rGCy'C
Cuts at: 1391 2077 2090 2975 1391
Size: 686 13 885 4072
Fragments arranged by size:
4072 885 686 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3847 3881 1903
Size: 34 50 34 1826 34 3678
Fragments arranged by size:
3678 1826 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4267 136
Size: 107 122 71 631 605 2595 1525
Fragments arranged by size:
2595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2461 2589 5651 2461
Size: 128 3062 2466
Fragments arranged by size:
3062 2466 128
BlpI GC'TnA_GC
Cuts at: 2490 3073 2490
Size: 583 5073
Bme1580I G_kGCm'C
Cuts at: 1790 2090 3717 4873 1790
Size: 300 1627 1156 2573
Fragments arranged by size:
2573 1627 1156 300
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2880 4316 1239
Size: 1097 544 1436 2579
Fragments arranged by size:
2579 1436 1097 544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 2887 4319 882
Size: 617 1186 202 1432 2219
Fragments arranged by size:
2219 1432 1186 617 202
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 2907 5650 2907
Size: 2743 2913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2905 4089 4487 4761 4908 5319 2905
Size: 1184 398 274 147 411 3242
Fragments arranged by size:
3242 1184 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2488 1186
Size: 880 422 4354
Fragments arranged by size:
4354 880 422
BseYI C'CCAG_C
Cuts at: 2167 2173 4859 5355 2167
Size: 6 2686 496 2468
Fragments arranged by size:
2686 2468 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 1163
Size: 573 342 4741
Fragments arranged by size:
4741 573 342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2090 2975 3717 3802 4873 1391
Size: 399 287 13 885 742 85 1071 2174
Fragments arranged by size:
2174 1071 885 742 399 287 85 13
BspEI T'CCGG_A
Cuts at: 2905 5319 2905
Size: 2414 3242
BspHI T'CATG_A
Cuts at: 2677 3545 5275 2677
Size: 868 1730 3058
Fragments arranged by size:
3058 1730 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 3011 844
Size: 1202 965 3489
Fragments arranged by size:
3489 1202 965
BsrBI CCG'CTC
Cuts at: 1394 1823 2142 3543 1394
Size: 429 319 1401 3507
Fragments arranged by size:
3507 1401 429 319
BsrDI GCAATG_nn'
Cuts at: 65 1248 2617 4151 4325 65
Size: 1183 1369 1534 174 1396
Fragments arranged by size:
1534 1396 1369 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 2081 3716 4728 2081
Size: 1635 1012 3009
Fragments arranged by size:
3009 1635 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2259 794
Size: 1465 4191
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2227 2461 2589 2902 2986 3353 3738 3755
Size: 255 234 128 313 84 367 385 17
Cuts at: 3755 5207 5293 5305 5651 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 313 255 234
128 86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2453 487
Size: 683 56 1227 3690
Fragments arranged by size:
3690 1227 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 3172 3966 3986 2257
Size: 915 794 20 3927
Fragments arranged by size:
3927 915 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2938 2960 2938
Size: 22 5634
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 3103 3992
Size: 54 1425 50 275 18 636 637 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 637 636 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2670 2880 3719 4316 5103 5371
Size: 393 704 334 210 839 597 787 268
Cuts at: 5371 1239
Size: 1524
Fragments arranged by size:
1524 839 787 704 597 393 334 268
210
EcoO109I rG'GnC_Cy
Cuts at: 2086 2087 2086
Size: 1 5655
EcoRI G'AATT_C
Cuts at: 2803 2810 2965 3343 2803
Size: 7 155 378 5116
Fragments arranged by size:
5116 378 155 7
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 2853 2885 4297
Size: 32 135 32 944 32 305 32 1412
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 944 305 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2480 748
Size: 1732 3924
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2569 2981 2569
Size: 412 5244
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 2619 2910 4770 4954 1305
Size: 1084 230 291 1860 184 2007
Fragments arranged by size:
2007 1860 1084 291 230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 10
Size: 54 1425 343 636 3198
Fragments arranged by size:
3198 1425 636 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2289 3614 3973 4132 518
Size: 648 984 139 1325 359 159 2042
Fragments arranged by size:
2042 1325 984 648 359 159 139
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 3014 4559 1132
Size: 97 1785 1545 2229
Fragments arranged by size:
2229 1785 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2793 1108
Size: 1685 3971
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2086 2086
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2192 3008 830
Size: 1362 816 3478
Fragments arranged by size:
3478 1362 816
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2188 2700 3004 4139 4820 5011 826
Size: 219 1143 512 304 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 681 512 304 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3760 4661 4923 820
Size: 197 926 1817 901 262 1553
Fragments arranged by size:
1817 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 2369 1517
Size: 852 4804
StyI C'CwwG_G
Cuts at: 513 1065 3095 513
Size: 552 2030 3074
Fragments arranged by size:
3074 2030 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI