(Circular) (Six-base) MAPSORT of: pFN21AE9402.seq Check: 8202 from: 1 to: 5707 pFN21AE9402 5707 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2573 2573 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2309 2630 3770 5154 5422 1632 Size: 407 270 321 1140 1384 268 1917 Fragments arranged by size: 1917 1384 1140 407 321 270 268 AfeI AGC'GCT Cuts at: 2937 2937 Size: 5707 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2123 3450 5022 2123 Size: 1327 1572 2808 Fragments arranged by size: 2808 1572 1327 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2097 2164 3032 1870 Size: 73 100 54 67 868 4545 Fragments arranged by size: 4545 868 100 73 67 54 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 2130 3026 1391 Size: 739 896 4072 Fragments arranged by size: 4072 896 739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 928 Size: 971 120 4616 Fragments arranged by size: 4616 971 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2661 5702 2661 Size: 3041 2666 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 2724 1318 Size: 1406 4301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 3438 4396 Size: 416 53 83 399 24 1574 447 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1574 958 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 1239 Size: 3128 2579 Bpu10I CC'TnA_GC Cuts at: 2124 2124 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2758 4370 882 Size: 617 1259 1612 2219 Fragments arranged by size: 2219 1612 1259 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 1186 Size: 897 4810 BseYI C'CCAG_C Cuts at: 2222 4910 5406 2222 Size: 2688 496 2523 Fragments arranged by size: 2688 2523 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2355 3157 3918 4946 1540 Size: 275 186 354 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 354 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 2236 3768 3853 4924 2130 Size: 106 1532 85 1071 2913 Fragments arranged by size: 2913 1532 1071 106 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2547 2547 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2236 3026 3768 3853 4924 1391 Size: 399 340 106 790 742 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 399 340 106 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 2328 2573 3062 844 Size: 1439 45 245 489 3489 Fragments arranged by size: 3489 1439 489 245 45 BsrBI CCG'CTC Cuts at: 1394 1823 2584 3594 1394 Size: 429 761 1010 3507 Fragments arranged by size: 3507 1010 761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2282 4351 4538 1700 Size: 110 472 2069 187 2869 Fragments arranged by size: 2869 2069 472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 2987 1925 Size: 1062 4645 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2344 2515 3004 2344 Size: 171 489 5047 Fragments arranged by size: 5047 489 171 BstYI r'GATC_y Cuts at: 1972 2661 2925 3037 3404 3789 3806 5258 5344 Size: 689 264 112 367 385 17 1452 86 Cuts at: 5344 5356 5702 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 689 385 367 346 264 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2021 4714 809 Size: 507 705 2693 1802 Fragments arranged by size: 2693 1802 705 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2601 2718 Size: 54 1425 50 275 18 450 321 117 Cuts at: 2718 3154 4043 5672 8 Size: 436 889 1629 43 Fragments arranged by size: 1629 1425 889 450 436 321 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 3637 1353 Size: 1277 1007 3423 Fragments arranged by size: 3423 1277 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2128 2128 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2309 2630 3770 4367 5154 5422 Size: 393 407 270 321 1140 597 787 268 Cuts at: 5422 1239 Size: 1524 Fragments arranged by size: 1524 1140 787 597 407 393 321 270 268 EcoO109I rG'GnC_Cy Cuts at: 2502 2502 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2939 4854 1678 Size: 1261 1915 2531 Fragments arranged by size: 2531 1915 1261 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 2413 3051 3297 669 Size: 988 281 199 276 638 246 3079 Fragments arranged by size: 3079 988 638 281 276 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 10 Size: 54 1425 343 888 2997 Fragments arranged by size: 2997 1425 888 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 3123 3800 4948 5193 1606 Size: 457 1060 677 1148 245 2120 Fragments arranged by size: 2120 1148 1060 677 457 245 NaeI GCC'GGC Cuts at: 2284 2284 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5155 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2282 2282 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 2142 1348 Size: 794 4913 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 1108 Size: 1608 4099 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2502 2502 Size: 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 2393 2570 2706 3059 830 Size: 1563 177 136 353 3478 Fragments arranged by size: 3478 1563 353 177 136 PvuI CG_AT'CG Cuts at: 2001 2355 2001 Size: 354 5353 SacI G_AGCT'C Cuts at: 2130 2130 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 2389 2566 2702 3055 4190 4871 5062 Size: 219 1344 177 136 353 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1344 1135 681 353 219 191 177 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2099 3034 1872 Size: 227 935 4545 Fragments arranged by size: 4545 935 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 3146 513 Size: 552 2081 3074 Fragments arranged by size: 3074 2081 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2251 3734 3751 3904 4089 4534 325 Size: 1255 671 1483 17 153 185 445 1498 Fragments arranged by size: 1498 1483 1255 671 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2430 3953 5665 Size: 274 80 33 51 1443 453 1523 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1523 1443 453 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2226 2258 5667 5699 2226 Size: 32 3409 32 2234 Fragments arranged by size: 3409 2234 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2150 1316 Size: 834 4873 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2569 2569 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2097 3032 1870 Size: 227 935 4545 Fragments arranged by size: 4545 935 227 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI