################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AspI AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Cac8I CelII CfoI ClaI CpoI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 48 GGTACC G^GTACC AhdI 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 8 CCTTAGG CC^T[ACGT]AGG Asp700I 24 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 48 GGTACC G^GTACC AspEI 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 130 GGGCTC G[GA]GC[CT]^C BbrPI 463 CACGTG CAC^GTG BbvI 192 GCAGC GCAGC(8/12) BcnI 87 CCCGG CC^[GC]GG BglI 269 GCCGATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 37 GTGCAG GTGCAG(16/14) BsrFI 75 GCCGGT [GA]^CCGG[CT] BstBI 418 TTCGAA TT^CGAA Bsu36I 8 CCTTAGG CC^T[ACGT]AGG Cfr10I 75 GCCGGT [GA]^CCGG[CT] DraI 168 TTTAAA TTT^AAA Eam1105I 143 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 8 CCTTAGG CC^T[ACGT]AGG FauI 145 CCCGC CCCGC(4/6) Fnu4HI 192 GCAGC GC^[ACGT]GC HaeIII 307 GGCC GG^CC HphI 13 GGTGA GGTGA(8/7) ItaI 192 GCAGC GC^[ACGT]GC KpnI 48 GGTACC GGTAC^C NciI 87 CCCGG CC^[GC]GG NspV 418 TTCGAA TT^CGAA PleI 338 GACTC GAGTC(4/5) PmaCI 463 CACGTG CAC^GTG PmlI 463 CACGTG CAC^GTG PstI 201 CTGCAG CTGCA^G SfaNI 43 GATGC GCATC(5/9) SfcI 201 CTGCAG C^T[GA][CT]AG SfuI 418 TTCGAA TT^CGAA SnaBI 438 TACGTA TAC^GTA TfiI 177 GATTC G^A[AT]TC TseI 192 GCAGC G^C[AT]GC XcmI 264 CCACAGCCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 24 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 286 GAATTT [GA]^AATT[CT] 547 AAATTC AluI 194 AGCT AG^CT 368 AGCT AlwI 162 GGATC GGATC(4/5) 376 GGATC ApoI 286 GAATTT [GA]^AATT[CT] 547 AAATTC AvaI 346 CTCGGG C^[CT]CG[GA]G 513 CCCGAG BanI 48 GGTACC G^G[CT][GA]CC 539 GGCACC BfaI 527 CTAG C^TAG 566 CTAG BmyI 64 GTGCCC G[AGT]GC[ACT]^C 130 GGGCTC BsaAI 438 TACGTA [CT]AC^GT[GA] 463 CACGTG BsiYI 119 CCGAAGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 514 CCGAGAGAAGG BslI 119 CCGAAGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 514 CCGAGAGAAGG BsmI 278 GCATTC GAATGC(1/-1) 317 GCATTC BsoBI 346 CTCGGG C^[CT]CG[GA]G 513 CCCGAG Bsp1286I 64 GTGCCC G[AGT]GC[ACT]^C 130 GGGCTC BstF5I 42 GGATG GGATG(2/0) 350 GGATG DpnII 163 GATC ^GATC 377 GATC DraII 141 GGGACCC [GA]G^G[ACGT]CC[CT] 508 GGGACCC EcoO109I 141 GGGACCC [GA]G^G[ACGT]CC[CT] 508 GGGACCC FokI 42 GGATG GGATG(9/13) 350 GGATG HapII 76 CCGG C^CGG 88 CCGG HinfI 177 GATTC G^A[ACGT]TC 338 GACTC HpaII 76 CCGG C^CGG 88 CCGG MaeI 527 CTAG C^TAG 566 CTAG MaeII 439 ACGT A^CGT 464 ACGT MaeIII 101 GTGAC ^GT[ACGT]AC 151 GTCAC MboI 163 GATC ^GATC 377 GATC MboII 421 GAAGA GAAGA(8/7) 227 TCTTC MspI 76 CCGG C^CGG 88 CCGG NdeII 163 GATC ^GATC 377 GATC PpuMI 141 GGGACCC [GA]G^G[AT]CC[CT] 508 GGGACCC Sau3AI 163 GATC ^GATC 377 GATC TaqI 321 TCGA T^CGA 419 TCGA Tsp45I 101 GTGAC ^GT[GC]AC 151 GTCAC Tsp509I 287 AATT ^AATT 548 AATT TthHB8I 321 TCGA T^CGA 419 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AfaI AvaII BsaJI BsmFI BsrI BssKI BstNI Cfr13I DdeI EcoRII MnlI MseI MvaI MwoI NlaIV RsaI Sau96I ScrFI Tru9I TspRI