################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BciVI BfrI BlnI BsaBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I ClaI DraI DrdI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI MamI MfeI MluI MslI MunI NdeI NheI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 562 GGTACC G^GTACC Asp718I 562 GGTACC G^GTACC BbvCI 1347 GCTGAGG CCTCAGC(-5/-2) BclI 2774 TGATCA T^GATCA BfaI 2569 CTAG C^TAG BglI 1117 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2397 AGATCT A^GATCT BsaI 844 GAGACC GGTCTC(1/5) BsiWI 629 CGTACG C^GTACG BsmBI 1754 GAGACG CGTCTC(1/5) BsrBI 558 GAGCGG CCGCTC(-3/-3) BstBI 517 TTCGAA TT^CGAA CpoI 1248 CGGTCCG CG^G[AT]CCG DraIII 1324 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 3115 GAATTC G^AATTC FbaI 2774 TGATCA T^GATCA HincII 1861 GTTAAC GT[CT]^[GA]AC HindII 1861 GTTAAC GT[CT]^[GA]AC HpaI 1861 GTTAAC GTT^AAC KpnI 562 GGTACC GGTAC^C MaeI 2569 CTAG C^TAG NcoI 2987 CCATGG C^CATGG NotI 762 GCGGCCGC GC^GGCCGC NspV 517 TTCGAA TT^CGAA PpuMI 2834 GGGACCT [GA]G^G[AT]CC[CT] PshAI 1296 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1248 CGGTCCG CG^G[AT]CCG SapI 1332 GAAGAGC GCTCTTC(1/4) SfuI 517 TTCGAA TT^CGAA SphI 1467 GCATGC GCATG^C SspI 2468 AATATT AAT^ATT StuI 3059 AGGCCT AGG^CCT XcmI 339 CCAGGAGATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 2273 TCCGGA T^CCGGA 2945 TCCGGA AcsI 3000 GAATTT [GA]^AATT[CT] 3115 GAATTC AocI 2094 CCTAAGG CC^T[ACGT]AGG 2824 CCTGAGG ApoI 3000 GAATTT [GA]^AATT[CT] 3115 GAATTC AviII 1571 TGCGCA TGC^GCA 1609 TGCGCA BalI 1892 TGGCCA TGG^CCA 2330 TGGCCA BbrPI 1177 CACGTG CAC^GTG 1825 CACGTG BseAI 2273 TCCGGA T^CCGGA 2945 TCCGGA BsgI 2436 CTGCAC GTGCAG(16/14) 805 GTGCAG BspEI 2273 TCCGGA T^CCGGA 2945 TCCGGA BspMI 770 ACCTGC ACCTGC(4/8) 1562 ACCTGC BstXI 146 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 602 CCACGGCGCTGG BstYI 1937 AGATCC [GA]^GATC[CT] 2397 AGATCT Bsu36I 2094 CCTAAGG CC^T[ACGT]AGG 2824 CCTGAGG Eco81I 2094 CCTAAGG CC^T[ACGT]AGG 2824 CCTGAGG EcoNI 2157 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3122 CCTTCCGAAGG FspI 1571 TGCGCA TGC^GCA 1609 TGCGCA HindIII 582 AAGCTT A^AGCTT 3185 AAGCTT MflI 1937 AGATCC [GA]^GATC[CT] 2397 AGATCT MluNI 1892 TGGCCA TGG^CCA 2330 TGGCCA MroI 2273 TCCGGA T^CCGGA 2945 TCCGGA MscI 1892 TGGCCA TGG^CCA 2330 TGGCCA NspI 984 GCATGT [GA]CATG^[CT] 1467 GCATGC PmaCI 1177 CACGTG CAC^GTG 1825 CACGTG PmlI 1177 CACGTG CAC^GTG 1825 CACGTG SacI 356 GAGCTC GAGCT^C 3201 GAGCTC SmlI 228 CTTGAG C^T[CT][GA]AG 361 CTCAAG XhoII 1937 AGATCC [GA]^GATC[CT] 2397 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcgI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I