(Circular) (Six-base) MAPSORT of: pFN21AE3368.seq Check: 6864 from: 1 to: 7969 pFN21AE3368 7969 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2784 3576 2784 Size: 792 7177 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5779 278 Size: 53 83 186 936 4243 2468 Fragments arranged by size: 4243 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5312 1937 Size: 3375 4594 Acc65I G'GTAC_C Cuts at: 2567 5290 2567 Size: 2723 5246 AclI AA'CG_TT Cuts at: 1563 6096 6469 1563 Size: 4533 373 3063 Fragments arranged by size: 4533 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4586 4793 4906 4925 5163 5265 6032 7416 Size: 2954 207 113 19 238 102 767 1384 Cuts at: 7416 7684 1632 Size: 268 1917 Fragments arranged by size: 2954 1917 1384 767 268 238 207 113 102 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7772 AflIII A'CryG_T Cuts at: 1203 6868 7845 1203 Size: 5665 977 1327 Fragments arranged by size: 5665 1327 977 AgeI A'CCGG_T Cuts at: 6800 6800 Size: 7969 AhdI GACnn_n'nnGTC Cuts at: 6698 6698 Size: 7969 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7969 AlwNI CAG_nnn'CTG Cuts at: 2059 2323 3043 5712 7284 2059 Size: 264 720 2669 1572 2744 Fragments arranged by size: 2744 2669 1572 720 264 ApaI G_GGCC'C Cuts at: 2585 2676 3384 3647 2585 Size: 91 708 263 6907 Fragments arranged by size: 6907 708 263 91 ApaLI G'TGCA_C Cuts at: 6026 7182 6026 Size: 1156 6813 ApoI r'AATT_y Cuts at: 1493 5005 5120 5278 5504 5656 7765 1493 Size: 3512 115 158 226 152 2109 1697 Fragments arranged by size: 3512 2109 1697 226 158 152 115 AseI AT'TA_AT Cuts at: 160 1032 6523 160 Size: 872 5491 1606 Fragments arranged by size: 5491 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 AvaI C'yCGr_G Cuts at: 1870 1943 2434 3656 4649 5294 1870 Size: 73 491 1222 993 645 4545 Fragments arranged by size: 4545 1222 993 645 491 73 BamHI G'GATC_C Cuts at: 5299 5666 5299 Size: 367 7602 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 2567 2711 2782 2900 Size: 325 217 627 492 288 144 71 118 Cuts at: 2900 3166 3400 3497 3540 4034 4605 4747 5290 Size: 266 234 97 43 494 571 142 543 Cuts at: 5290 6745 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 571 543 494 492 325 288 266 234 217 144 142 118 97 71 43 BanII G_rGCy'C Cuts at: 1391 2365 2490 2585 2676 2937 3002 3276 3384 Size: 974 125 95 91 261 65 274 108 Cuts at: 3384 3647 3657 4518 4829 5210 5288 1391 Size: 263 10 861 311 381 78 4072 Fragments arranged by size: 4072 974 861 381 311 274 263 261 125 108 95 91 78 65 10 BbeI G_GCGC'C Cuts at: 2904 3170 3501 2904 Size: 266 331 7372 Fragments arranged by size: 7372 331 266 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 4100 4282 4574 928 Size: 971 192 2009 182 292 4323 Fragments arranged by size: 4323 2009 971 292 192 182 BbvCI CC'TCA_GC Cuts at: 3353 3353 Size: 7969 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2051 2085 2371 2405 2525 2559 3550 Size: 34 114 34 286 34 120 34 991 Cuts at: 3550 3584 6160 6194 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 991 286 120 114 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5861 7071 5861 Size: 1210 6759 BclI T'GATC_A Cuts at: 1548 4779 1548 Size: 3231 4738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3128 6580 136 Size: 107 122 71 631 605 1456 3452 1525 Fragments arranged by size: 3452 1525 1456 631 605 122 107 71 BglII A'GATC_T Cuts at: 4402 7964 4402 Size: 3562 4407 BlpI GC'TnA_GC Cuts at: 2561 3921 4823 5386 2561 Size: 1360 902 563 5144 Fragments arranged by size: 5144 1360 902 563 Bme1580I G_kGCm'C Cuts at: 1790 2282 2585 2676 2737 3213 3384 3405 3545 Size: 492 303 91 61 476 171 21 140 Cuts at: 3545 3647 6030 7186 1790 Size: 102 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 492 476 303 171 140 102 91 61 21 BmgBI CAC'GTC Cuts at: 1318 3514 4141 1318 Size: 2196 627 5146 Fragments arranged by size: 5146 2196 627 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 3200 4455 Size: 416 53 83 399 24 1078 705 1255 Cuts at: 4455 4795 5700 6658 442 Size: 340 905 958 1753 Fragments arranged by size: 1753 1255 1078 958 905 705 416 399 340 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7969 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4727 Size: 7969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2636 3182 3905 4924 6629 1239 Size: 1184 213 546 723 1019 1705 2579 Fragments arranged by size: 2579 1705 1184 1019 723 546 213 Bpu10I CC'TnA_GC Cuts at: 3353 4296 5103 3353 Size: 943 807 6219 Fragments arranged by size: 6219 943 807 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2254 2351 6094 6959 7257 2254 Size: 97 3743 865 298 2966 Fragments arranged by size: 3743 2966 865 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2843 6632 882 Size: 617 1344 3789 2219 Fragments arranged by size: 3789 2219 1344 617 BsaAI yAC'GTr Cuts at: 493 1204 2638 2941 3184 3832 5266 5760 493 Size: 711 1434 303 243 648 1434 494 2702 Fragments arranged by size: 2702 1434 1434 711 648 494 303 243 BsaBI GATnn'nnATC Cuts at: 7963 7963 Size: 7969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2840 2901 3167 3459 Size: 53 83 186 936 1307 61 266 292 Cuts at: 3459 3498 5776 6158 6793 275 Size: 39 2278 382 635 1451 Fragments arranged by size: 2278 1451 1307 936 635 382 292 266 186 83 61 53 39 BsaWI w'CCGG_w Cuts at: 3534 3870 4122 4278 4950 6402 6800 7074 7221 Size: 336 252 156 672 1452 398 274 147 Cuts at: 7221 7632 3534 Size: 411 3871 Fragments arranged by size: 3871 1452 672 411 398 336 274 252 156 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7744 Fragments arranged by size: 7744 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 3482 4649 1186 Size: 1447 849 1167 4506 Fragments arranged by size: 4506 1447 1167 849 BseYI C'CCAG_C Cuts at: 2516 2995 3212 3484 4517 4767 7172 7668 2516 Size: 479 217 272 1033 250 2405 496 2817 Fragments arranged by size: 2817 2405 1033 496 479 272 250 217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 4426 1163 Size: 573 1095 1595 4706 Fragments arranged by size: 4706 1595 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2633 2771 3315 5419 6180 7208 Size: 275 186 632 138 544 2104 761 1028 Cuts at: 7208 1540 Size: 2301 Fragments arranged by size: 2301 2104 1028 761 632 544 275 186 138 BsiHKAI G_wGCw'C Cuts at: 2365 4302 4659 5109 5210 6030 6115 7186 2365 Size: 1937 357 450 101 820 85 1071 3148 Fragments arranged by size: 3148 1937 1071 820 450 357 101 85 BsiWI C'GTAC_G Cuts at: 2634 2634 Size: 7969 BsmI GAATG_Cn' Cuts at: 5480 5573 5480 Size: 93 7876 BsmBI CGTCTCn'nnnn_ Cuts at: 3753 3753 Size: 7969 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 2365 2490 2585 2676 2737 2937 Size: 399 492 83 125 95 91 61 200 Cuts at: 2937 3002 3213 3276 3384 3405 3545 3647 3657 Size: 65 211 63 108 21 140 102 10 Cuts at: 3657 4302 4518 4659 4829 5109 5210 5288 6030 Size: 645 216 141 170 280 101 78 742 Cuts at: 6030 6115 7186 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 645 492 399 280 216 211 200 170 141 140 125 108 102 101 95 91 85 83 78 65 63 61 21 10 BspEI T'CCGG_A Cuts at: 4278 4950 7632 4278 Size: 672 2682 4615 Fragments arranged by size: 4615 2682 672 BspHI T'CATG_A Cuts at: 5858 7588 5858 Size: 1730 6239 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2784 3576 5324 844 Size: 1940 792 1748 3489 Fragments arranged by size: 3489 1940 1748 792 BsrBI CCG'CTC Cuts at: 1394 1823 2565 5856 1394 Size: 429 742 3291 3507 Fragments arranged by size: 3507 3291 742 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6464 6638 65 Size: 1183 5216 174 1396 Fragments arranged by size: 5216 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 2304 2531 2903 2966 3115 6613 Size: 110 239 255 227 372 63 149 3498 Cuts at: 6613 6800 1700 Size: 187 2869 Fragments arranged by size: 3498 2869 372 255 239 227 187 149 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7969 BssHII G'CGCG_C Cuts at: 1925 2754 3175 3177 1925 Size: 829 421 2 6717 Fragments arranged by size: 6717 829 421 2 BssSI C'ACGA_G Cuts at: 6029 7041 6029 Size: 1012 6957 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 2314 2452 3043 3325 3571 794 Size: 1265 255 138 591 282 246 5192 Fragments arranged by size: 5192 1265 591 282 255 246 138 BstBI TT'CG_AA Cuts at: 2523 6773 2523 Size: 4250 3719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7969 BstXI CCAn_nnnn'nTGG Cuts at: 2158 2614 2158 Size: 456 7513 BstYI r'GATC_y Cuts at: 1972 3942 4402 5299 5666 6051 6068 7520 7606 Size: 1970 460 897 367 385 17 1452 86 Cuts at: 7606 7618 7964 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1970 1452 897 460 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4100 4830 4100 Size: 730 7239 BtgI C'CryG_G Cuts at: 513 1065 2607 2627 2668 2973 3245 4992 513 Size: 552 1542 20 41 305 272 1747 3490 Fragments arranged by size: 3490 1747 1542 552 305 272 41 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4303 4627 487 Size: 683 56 3077 324 3829 Fragments arranged by size: 3829 3077 683 324 56 BtsI GCAGTG_nn' Cuts at: 3116 5485 6279 6299 3116 Size: 2369 794 20 4786 Fragments arranged by size: 4786 2369 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7969 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7934 DraI TTT'AAA Cuts at: 5273 5273 Size: 7969 DraIII CAC_nnn'GTG Cuts at: 3334 3334 Size: 7969 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6976 809 Size: 507 5660 1802 Fragments arranged by size: 5660 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2356 2630 2677 Size: 54 1425 50 275 18 526 274 47 Cuts at: 2677 2768 3113 3312 3897 4164 4335 5416 6305 Size: 91 345 199 585 267 171 1081 889 Cuts at: 6305 7934 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1081 889 585 526 345 275 274 267 199 171 91 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2630 2768 3312 5416 1537 Size: 275 818 138 544 2104 4090 Fragments arranged by size: 4090 2104 818 544 275 138 EarI CTCTTCn'nnn_ Cuts at: 1353 3332 3759 3764 4718 5899 1353 Size: 1979 427 5 954 1181 3423 Fragments arranged by size: 3423 1979 1181 954 427 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3246 3540 3717 4900 6556 6930 Size: 1064 138 1657 294 177 1183 1656 374 Cuts at: 6930 7076 387 Size: 146 1280 Fragments arranged by size: 1657 1656 1280 1183 1064 374 294 177 146 138 EcoICRI GAG'CTC Cuts at: 2363 5208 2363 Size: 2845 5124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2636 3182 3905 4586 4793 4906 Size: 393 791 213 546 723 681 207 113 Cuts at: 4906 4924 4925 5163 5265 6032 6629 7416 7684 Size: 18 1 238 102 767 597 787 268 Cuts at: 7684 1239 Size: 1524 Fragments arranged by size: 1524 791 787 767 723 681 597 546 393 268 238 213 207 113 102 18 1 EcoNI CCTnn'n_nnAGG Cuts at: 4166 5131 4166 Size: 965 7004 EcoO109I rG'GnC_Cy Cuts at: 2581 3380 3381 3644 4840 2581 Size: 799 1 263 1196 5710 Fragments arranged by size: 5710 1196 799 263 1 EcoRI G'AATT_C Cuts at: 5120 5278 5656 5120 Size: 158 378 7433 Fragments arranged by size: 7433 378 158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4070 4378 4070 Size: 308 7661 FspI TGC'GCA Cuts at: 3578 3616 6475 3578 Size: 38 2859 5072 Fragments arranged by size: 5072 2859 38 HaeII r_GCGC'y Cuts at: 1678 2598 2615 2904 3170 3485 3501 3687 4929 Size: 920 17 289 266 315 16 186 1242 Cuts at: 4929 7116 1678 Size: 2187 2531 Fragments arranged by size: 2531 2187 1242 920 315 289 266 186 17 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4523 4555 6610 6642 6684 Size: 32 135 32 2951 32 2055 32 42 Cuts at: 6684 6716 1373 Size: 32 2626 Fragments arranged by size: 2951 2626 2055 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3868 5313 5559 669 Size: 988 281 1930 1445 246 3079 Fragments arranged by size: 3079 1930 1445 988 281 246 HindIII A'AGCT_T Cuts at: 748 2587 5190 748 Size: 1839 2603 3527 Fragments arranged by size: 3527 2603 1839 HpaI GTT'AAC Cuts at: 3868 5559 3868 Size: 1691 6278 KasI G'GCGC_C Cuts at: 2900 3166 3497 2900 Size: 266 331 7372 Fragments arranged by size: 7372 331 266 KpnI G_GTAC'C Cuts at: 2571 5294 2571 Size: 2723 5246 MfeI C'AATT_G Cuts at: 5568 5568 Size: 7969 MluI A'CGCG_T Cuts at: 7845 7845 Size: 7969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3567 4681 7083 7267 1305 Size: 2262 1114 2402 184 2007 Fragments arranged by size: 2402 2262 2007 1114 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3899 4337 10 Size: 54 1425 343 2067 438 3642 Fragments arranged by size: 3642 2067 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5927 6286 6445 518 Size: 648 4761 359 159 2042 Fragments arranged by size: 4761 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2291 2560 2629 2746 2975 2986 3247 3508 Size: 685 269 69 117 229 11 261 261 Cuts at: 3508 3883 4771 5385 6062 7210 7455 1606 Size: 375 888 614 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 685 677 614 375 269 261 261 245 229 117 69 11 NaeI GCC'GGC Cuts at: 2051 2905 3117 2051 Size: 854 212 6903 Fragments arranged by size: 6903 854 212 NarI GG'CG_CC Cuts at: 2901 3167 3498 2901 Size: 266 331 7372 Fragments arranged by size: 7372 331 266 NcoI C'CATG_G Cuts at: 513 1065 4992 513 Size: 552 3927 3490 Fragments arranged by size: 3927 3490 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7969 NgoMIV G'CCGG_C Cuts at: 2049 2903 3115 2049 Size: 854 212 6903 Fragments arranged by size: 6903 854 212 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7969 NotI GC'GGCC_GC Cuts at: 2768 5416 2768 Size: 2648 5321 NspI r_CATG'y Cuts at: 1132 1229 2993 3476 5327 6872 1132 Size: 97 1764 483 1851 1545 2229 Fragments arranged by size: 2229 1851 1764 1545 483 97 PasI CC'CwG_GG Cuts at: 1348 2797 4024 1348 Size: 1449 1227 5293 Fragments arranged by size: 5293 1449 1227 PciI A'CATG_T Cuts at: 6868 6868 Size: 7969 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 4357 4879 4993 5067 1108 Size: 1242 2007 522 114 74 4010 Fragments arranged by size: 4010 2007 1242 522 114 74 PmeI GTTT'AAAC Cuts at: 5273 5273 Size: 7969 PmlI CAC'GTG Cuts at: 3184 3832 3184 Size: 648 7321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6038 6070 7551 7583 6038 Size: 32 1481 32 6424 Fragments arranged by size: 6424 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4840 4840 Size: 7969 PshAI GACnn'nnGTC Cuts at: 1613 3305 1613 Size: 1692 6277 PsiI TTA'TAA Cuts at: 5539 5539 Size: 7969 PspOMI G'GGCC_C Cuts at: 2581 2672 3380 3643 2581 Size: 91 708 263 6907 Fragments arranged by size: 6907 708 263 91 PstI C_TGCA'G Cuts at: 830 2290 2296 2319 2751 3150 3882 5321 830 Size: 1460 6 23 432 399 732 1439 3478 Fragments arranged by size: 3478 1460 1439 732 432 399 23 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7969 PvuII CAG'CTG Cuts at: 2291 2560 2986 3883 4771 2291 Size: 269 426 897 888 5489 Fragments arranged by size: 5489 897 888 426 269 RsrII CG'GwC_CG Cuts at: 3254 3254 Size: 7969 SacI G_AGCT'C Cuts at: 2365 5210 2365 Size: 2845 5124 SacII CC_GC'GG Cuts at: 2630 2976 3248 2630 Size: 346 272 7351 Fragments arranged by size: 7351 346 272 SalI G'TCGA_C Cuts at: 1936 5311 1936 Size: 3375 4594 SapI GCTCTTCn'nnn_ Cuts at: 3332 3332 Size: 7969 SbfI CC_TGCA'GG Cuts at: 5321 5321 Size: 7969 ScaI AGT'ACT Cuts at: 6217 7929 6217 Size: 1712 6257 SfcI C'TryA_G Cuts at: 826 1045 2286 2292 2315 2747 3146 3878 5317 Size: 219 1241 6 23 432 399 732 1439 Cuts at: 5317 6452 7133 7324 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1439 1241 1135 732 681 432 399 219 191 23 6 SfoI GGC'GCC Cuts at: 2902 3168 3499 2902 Size: 266 331 7372 Fragments arranged by size: 7372 331 266 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7969 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7969 SmaI CCC'GGG Cuts at: 1872 5296 1872 Size: 3424 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2233 2366 6073 6974 7236 820 Size: 197 926 290 133 3707 901 262 1553 Fragments arranged by size: 3707 1553 926 901 290 262 197 133 SnaBI TAC'GTA Cuts at: 493 5760 493 Size: 5267 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7969 SphI G_CATG'C Cuts at: 1132 3476 5327 1132 Size: 2344 1851 3774 Fragments arranged by size: 3774 2344 1851 SspI AAT'ATT Cuts at: 5 52 4475 5893 5 Size: 47 4423 1418 2081 Fragments arranged by size: 4423 2081 1418 47 StuI AGG'CCT Cuts at: 1517 5066 1517 Size: 3549 4420 StyI C'CwwG_G Cuts at: 513 1065 2700 3462 3687 4992 5033 5061 5408 Size: 552 1635 762 225 1305 41 28 347 Cuts at: 5408 513 Size: 3074 Fragments arranged by size: 3074 1635 1305 762 552 347 225 41 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2709 2713 2964 3388 4174 5996 6013 Size: 1255 1129 4 251 424 786 1822 17 Cuts at: 6013 6166 6351 6796 325 Size: 153 185 445 1498 Fragments arranged by size: 1822 1498 1255 1129 786 445 424 251 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 4227 6215 Size: 274 80 33 51 1443 429 1821 1988 Cuts at: 6215 7927 96 Size: 1712 138 Fragments arranged by size: 1988 1821 1712 1443 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3596 3628 7929 7961 3596 Size: 32 4301 32 3604 Fragments arranged by size: 4301 3604 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7969 XbaI T'CTAG_A Cuts at: 5305 5305 Size: 7969 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2351 Size: 7969 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7969 XmaI C'CCGG_G Cuts at: 1870 5294 1870 Size: 3424 4545 XmnI GAAnn'nnTTC Cuts at: 6098 6098 Size: 7969 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5777 276 Size: 53 83 186 936 4243 2468 Fragments arranged by size: 4243 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SanDI SexAI SfiI SrfI SwaI