################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BbrPI BfrI BglII BlnI BsiEI BsiWI BsmBI BsmI BspDI BsrDI BsrFI BssHII Bst1107I BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRV FseI FspI HindIII HpaI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 2308 GGTACC G^GTACC Aor51HI 1799 AGCGCT AGC^GCT Asp700I 2508 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2308 GGTACC G^GTACC AspI 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1873 CTCGAG C^[CT]CG[GA]G BamHI 158 GGATCC G^GATCC BbeI 2131 GGCGCC GGCGC^C BbvCI 912 CCTCAGC CCTCAGC(-5/-2) BcgI 2186 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1982 TGATCA T^GATCA BfaI 353 CTAG C^TAG BlpI 2565 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2565 GCTCAGC GC^T[ACGT]AGC BsaAI 1475 CACGTA [CT]AC^GT[GA] BsaBI 2055 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2249 GAGACC GGTCTC(1/5) BseRI 1128 CTCCTC GAGGAG(10/8) BsoBI 1873 CTCGAG C^[CT]CG[GA]G Bsp1407I 440 TGTACA T^GTACA BspLU11I 2366 ACATGT A^CATGT BsrBI 694 GAGCGG CCGCTC(-3/-3) BsrGI 440 TGTACA T^GTACA BstAPI 2102 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2276 CCAAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2565 GCTCAGC GC^T[ACGT]AGC DraIII 1684 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1799 AGCGCT AGC^GCT EcoT22I 1345 ATGCAT ATGCA^T FbaI 1982 TGATCA T^GATCA KasI 2131 GGCGCC G^GCGCC KpnI 2308 GGTACC GGTAC^C MaeI 353 CTAG C^TAG MamI 2055 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2106 CAATTG C^AATTG MseI 973 TTAA T^TAA MunI 2106 CAATTG C^AATTG NarI 2131 GGCGCC GG^CGCC NsiI 1345 ATGCAT ATGCA^T PaeR7I 1873 CTCGAG C^TCGAG PflFI 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 2389 CTGCAG CTGCA^G SalI 2605 GTCGAC G^TCGAC SbfI 2388 CCTGCAGG CCTGCA^GG SexAI 1685 ACCAGGT A^CC[AT]GGT SfoI 2131 GGCGCC GGC^GCC Sse8387I 2388 CCTGCAGG CCTGCA^GG SspBI 440 TGTACA T^GTACA TfiI 954 GAATC G^A[AT]TC Tru9I 973 TTAA T^TAA Tth111I 1063 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 352 TCTAGA T^CTAGA XcmI 487 CCAGGCCCCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1873 CTCGAG C^TCGAG XmnI 2508 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 1179 GAATTC [GA]^AATT[CT] 2427 GAATTC AflIII 1474 ACACGT A^C[GA][CT]GT 2366 ACATGT ApoI 1179 GAATTC [GA]^AATT[CT] 2427 GAATTC BanI 2131 GGCGCC G^G[CT][GA]CC 2308 GGTACC BglI 482 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2134 GCCATCTGGGC BspHI 785 TCATGA T^CATGA 2528 TCATGA BstEII 1113 GGTCACC G^GT[ACGT]ACC 1755 GGTCACC BstPI 1113 GGTCACC G^GT[ACGT]ACC 1755 GGTCACC EcoO65I 1113 GGTCACC G^GT[ACGT]ACC 1755 GGTCACC EcoRI 1179 GAATTC G^AATTC 2427 GAATTC PshAI 501 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2202 GACCAGAGTC RcaI 785 TCATGA T^CATGA 2528 TCATGA SfcI 1971 CTACAG C^T[GA][CT]AG 2389 CTGCAG SmlI 1459 CTCAAG C^T[CT][GA]AG 1873 CTCGAG SphI 750 GCATGC GCATG^C 1289 GCATGC StuI 470 AGGCCT AGG^CCT 1238 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanII BbsI BbvI BciVI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseAI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspEI BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII