(Circular) (Six-base) MAPSORT of: pFN21AE9427.seq Check: 5368 from: 1 to: 7327 pFN21AE9427 7327 bp With 182 enzymes: SgfI * March 28, 2012 11:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3649 3649 Size: 7327 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5137 278 Size: 53 83 186 936 3601 2468 Fragments arranged by size: 3601 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 2550 4611 4670 1937 Size: 145 468 2061 59 4594 Fragments arranged by size: 4594 2061 468 145 59 Acc65I G'GTAC_C Cuts at: 4313 4648 4313 Size: 335 6992 AclI AA'CG_TT Cuts at: 1563 5454 5827 1563 Size: 3891 373 3063 Fragments arranged by size: 3891 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2331 2467 3532 5390 6774 7042 1632 Size: 527 172 136 1065 1858 1384 268 1917 Fragments arranged by size: 1917 1858 1384 1065 527 268 172 136 AfeI AGC'GCT Cuts at: 3806 3806 Size: 7327 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7130 AflIII A'CryG_T Cuts at: 1203 3479 4371 6226 7203 1203 Size: 2276 892 1855 977 1327 Fragments arranged by size: 2276 1855 1327 977 892 AgeI A'CCGG_T Cuts at: 6158 6158 Size: 7327 AhdI GACnn_n'nnGTC Cuts at: 6056 6056 Size: 7327 AleI CACnn'nnGTG Cuts at: 1166 3272 3582 1166 Size: 2106 310 4911 Fragments arranged by size: 4911 2106 310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7327 AlwNI CAG_nnn'CTG Cuts at: 2280 3433 3856 4402 5070 6642 2280 Size: 1153 423 546 668 1572 2965 Fragments arranged by size: 2965 1572 1153 668 546 423 ApaLI G'TGCA_C Cuts at: 5384 6540 5384 Size: 1156 6171 ApoI r'AATT_y Cuts at: 1493 3184 4432 4636 4862 5014 7123 1493 Size: 1691 1248 204 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 1248 226 204 152 AseI AT'TA_AT Cuts at: 160 1032 5881 160 Size: 872 4849 1606 Fragments arranged by size: 4849 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7327 AvaI C'yCGr_G Cuts at: 1870 1943 3878 4652 1870 Size: 73 1935 774 4545 Fragments arranged by size: 4545 1935 774 73 BamHI G'GATC_C Cuts at: 2163 4657 5024 2163 Size: 2494 367 4466 Fragments arranged by size: 4466 2494 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4136 4313 4648 6103 618 Size: 325 217 627 2349 177 335 1455 1842 Fragments arranged by size: 2349 1842 1455 627 335 325 217 177 BanII G_rGCy'C Cuts at: 1391 2707 3134 3443 4646 1391 Size: 1316 427 309 1203 4072 Fragments arranged by size: 4072 1316 1203 427 309 BbeI G_GCGC'C Cuts at: 4140 4140 Size: 7327 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2760 3514 928 Size: 971 446 415 754 4741 Fragments arranged by size: 4741 971 754 446 415 BbvCI CC'TCA_GC Cuts at: 2918 2918 Size: 7327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 5518 5552 1903 Size: 34 2243 34 1304 34 3678 Fragments arranged by size: 3678 2243 1304 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2173 2923 3097 5219 6429 2173 Size: 750 174 2122 1210 3071 Fragments arranged by size: 3071 2122 1210 750 174 BclI T'GATC_A Cuts at: 1548 3987 1548 Size: 2439 4888 BfrBI ATG'CAT Cuts at: 3352 3352 Size: 7327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 4145 5938 Size: 107 122 71 631 605 821 1652 1793 Cuts at: 5938 136 Size: 1525 Fragments arranged by size: 1793 1652 1525 821 631 605 122 107 71 BglII A'GATC_T Cuts at: 7322 7322 Size: 7327 BlpI GC'TnA_GC Cuts at: 4571 4744 4571 Size: 173 7154 Bme1580I G_kGCm'C Cuts at: 1790 3890 5388 6544 1790 Size: 2100 1498 1156 2573 Fragments arranged by size: 2573 2100 1498 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2922 4254 5058 Size: 416 53 83 399 24 1505 1332 804 Cuts at: 5058 6016 442 Size: 958 1753 Fragments arranged by size: 1753 1505 1332 958 804 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7327 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3151 3620 3151 Size: 469 6858 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2168 2208 2401 3283 3419 3692 5987 Size: 832 97 40 193 882 136 273 2295 Cuts at: 5987 1239 Size: 2579 Fragments arranged by size: 2579 2295 882 832 273 193 136 97 40 Bpu10I CC'TnA_GC Cuts at: 2567 2918 2567 Size: 351 6976 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3449 5452 6317 6615 3449 Size: 2003 865 298 4161 Fragments arranged by size: 4161 2003 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4248 5990 882 Size: 617 2749 1742 2219 Fragments arranged by size: 2749 2219 1742 617 BsaAI yAC'GTr Cuts at: 493 1204 3482 5118 493 Size: 711 2278 1636 2702 Fragments arranged by size: 2702 2278 1636 711 BsaBI GATnn'nnATC Cuts at: 4064 7321 4064 Size: 3257 4070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 2123 4137 5134 Size: 53 83 186 936 546 44 2014 997 Cuts at: 5134 5516 6151 275 Size: 382 635 1451 Fragments arranged by size: 2014 1451 997 936 635 546 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 2713 3554 3795 5760 6158 6432 6579 6990 2713 Size: 841 241 1965 398 274 147 411 3050 Fragments arranged by size: 3050 1965 841 411 398 274 241 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 2261 2291 3536 Size: 30 165 30 778 30 50 30 1245 Cuts at: 3536 3549 3566 3579 1178 Size: 13 17 13 4926 Fragments arranged by size: 4926 1245 778 165 50 30 30 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3124 1186 Size: 1938 5389 BseYI C'CCAG_C Cuts at: 6530 7026 6530 Size: 496 6831 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3083 3610 3953 1163 Size: 573 1347 527 343 4537 Fragments arranged by size: 4537 1347 573 527 343 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4777 5538 6566 1540 Size: 275 186 2776 761 1028 2301 Fragments arranged by size: 2776 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2573 3269 3741 3791 3918 5388 5473 6544 2573 Size: 696 472 50 127 1470 85 1071 3356 Fragments arranged by size: 3356 1470 1071 696 472 127 85 50 BsmI GAATG_Cn' Cuts at: 4838 4931 4838 Size: 93 7234 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 2707 3134 3269 3443 3741 3791 Size: 399 783 134 427 135 174 298 50 Cuts at: 3791 3890 3918 4646 5388 5473 6544 1391 Size: 99 28 728 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 728 427 399 298 174 135 134 99 85 50 28 BspEI T'CCGG_A Cuts at: 2713 3554 3795 6990 2713 Size: 841 241 3195 3050 Fragments arranged by size: 3195 3050 841 241 BspHI T'CATG_A Cuts at: 2790 4533 5216 6946 2790 Size: 1743 683 1730 3171 Fragments arranged by size: 3171 1743 1730 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2256 3105 3649 4682 844 Size: 1412 849 544 1033 3489 Fragments arranged by size: 3489 1412 1033 849 544 BsrBI CCG'CTC Cuts at: 1394 1823 2701 5214 1394 Size: 429 878 2513 3507 Fragments arranged by size: 3507 2513 878 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5822 5996 65 Size: 1183 4574 174 1396 Fragments arranged by size: 4574 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5971 6158 1700 Size: 110 4161 187 2869 Fragments arranged by size: 4161 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2445 96 Size: 2349 4978 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7327 BssSI C'ACGA_G Cuts at: 2598 2627 3239 3592 4318 4483 5387 6399 2598 Size: 29 612 353 726 165 904 1012 3526 Fragments arranged by size: 3526 1012 904 726 612 353 165 29 BstAPI GCAn_nnn'nTGC Cuts at: 794 4113 794 Size: 3319 4008 BstBI TT'CG_AA Cuts at: 6131 6131 Size: 7327 BstEII G'GTnAC_C Cuts at: 1184 3118 3760 1184 Size: 1934 642 4751 Fragments arranged by size: 4751 1934 642 BstXI CCAn_nnnn'nTGG Cuts at: 4288 4288 Size: 7327 BstYI r'GATC_y Cuts at: 1972 2163 2994 3231 4245 4657 5024 5409 5426 Size: 191 831 237 1014 412 367 385 17 Cuts at: 5426 6878 6964 6976 7322 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1014 831 412 385 367 346 237 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2985 3039 3123 3141 3156 3211 Size: 552 939 981 54 84 18 15 55 Cuts at: 3211 3577 4086 4249 4623 513 Size: 366 509 163 374 3217 Fragments arranged by size: 3217 981 939 552 509 374 366 163 84 55 54 18 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4183 487 Size: 683 56 2957 3631 Fragments arranged by size: 3631 2957 683 56 BtsI GCAGTG_nn' Cuts at: 2306 2736 4293 4843 5637 5657 2306 Size: 430 1557 550 794 20 3976 Fragments arranged by size: 3976 1557 794 550 430 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7327 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7292 DraI TTT'AAA Cuts at: 4631 4631 Size: 7327 DraIII CAC_nnn'GTG Cuts at: 3694 3694 Size: 7327 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6334 809 Size: 507 5018 1802 Fragments arranged by size: 5018 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 3159 3750 Size: 54 1425 50 275 18 311 1018 591 Cuts at: 3750 3909 4290 4774 5663 7292 8 Size: 159 381 484 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 591 484 381 311 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4774 1537 Size: 275 2962 4090 Fragments arranged by size: 4090 2962 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2792 2853 4059 5257 1353 Size: 1439 61 1206 1198 3423 Fragments arranged by size: 3423 1439 1206 1198 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3863 4483 5914 6288 6434 387 Size: 1064 138 2274 620 1431 374 146 1280 Fragments arranged by size: 2274 1431 1280 1064 620 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2159 2168 2208 2331 2401 2467 Size: 393 439 88 9 40 123 70 66 Cuts at: 2467 3283 3419 3532 3692 5390 5987 6774 7042 Size: 816 136 113 160 1698 597 787 268 Cuts at: 7042 1239 Size: 1524 Fragments arranged by size: 1698 1524 816 787 597 439 393 268 160 136 123 113 88 70 66 40 9 EcoNI CCTnn'n_nnAGG Cuts at: 2231 3250 3656 2231 Size: 1019 406 5902 Fragments arranged by size: 5902 1019 406 EcoO109I rG'GnC_Cy Cuts at: 2495 2539 2562 3853 4542 2495 Size: 44 23 1291 689 5280 Fragments arranged by size: 5280 1291 689 44 23 EcoRI G'AATT_C Cuts at: 3184 4432 4636 5014 3184 Size: 1248 204 378 5497 Fragments arranged by size: 5497 1248 378 204 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2992 2992 Size: 7327 FspI TGC'GCA Cuts at: 5833 5833 Size: 7327 HaeII r_GCGC'y Cuts at: 1678 3039 3701 3808 4140 4233 6474 1678 Size: 1361 662 107 332 93 2241 2531 Fragments arranged by size: 2531 2241 1361 662 332 107 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2376 2408 3536 3549 3568 Size: 32 135 32 804 32 1128 13 19 Cuts at: 3568 3581 5968 6000 6042 6074 1373 Size: 13 2387 32 42 32 2626 Fragments arranged by size: 2626 2387 1128 804 135 42 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2557 2623 4183 4275 4345 Size: 988 281 274 345 66 1560 92 70 Cuts at: 4345 4612 4671 4917 669 Size: 267 59 246 3079 Fragments arranged by size: 3079 1560 988 345 281 274 267 246 92 70 66 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 7327 HpaI GTT'AAC Cuts at: 4917 4917 Size: 7327 KasI G'GCGC_C Cuts at: 4136 4136 Size: 7327 KpnI G_GTAC'C Cuts at: 4317 4652 4317 Size: 335 6992 MfeI C'AATT_G Cuts at: 4111 4926 4111 Size: 815 6512 MluI A'CGCG_T Cuts at: 7203 7203 Size: 7327 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 2993 3117 3984 4530 6441 6625 1305 Size: 1335 353 124 867 546 1911 184 2007 Fragments arranged by size: 2007 1911 1335 867 546 353 184 124 MscI TGG'CCA Cuts at: 10 64 1489 1832 2143 3161 3752 10 Size: 54 1425 343 311 1018 591 3585 Fragments arranged by size: 3585 1425 1018 591 343 311 54 MslI CAynn'nnrTG Cuts at: 518 1166 3074 3272 3582 3653 3659 4202 5285 Size: 648 1908 198 310 71 6 543 1083 Cuts at: 5285 5644 5803 518 Size: 359 159 2042 Fragments arranged by size: 2042 1908 1083 648 543 359 310 198 159 71 6 MspA1I CmG'CkG Cuts at: 1606 2421 3624 3670 4743 5420 6568 6813 1606 Size: 815 1203 46 1073 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 1073 815 677 245 46 NarI GG'CG_CC Cuts at: 4137 4137 Size: 7327 NcoI C'CATG_G Cuts at: 513 1065 2004 3141 4086 4249 513 Size: 552 939 1137 945 163 3591 Fragments arranged by size: 3591 1137 945 939 552 163 NdeI CA'TA_TG Cuts at: 387 387 Size: 7327 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7327 NotI GC'GGCC_GC Cuts at: 4774 4774 Size: 7327 NsiI A_TGCA'T Cuts at: 3354 3354 Size: 7327 NspI r_CATG'y Cuts at: 1132 1229 2759 3298 4375 4685 6230 1132 Size: 97 1530 539 1077 310 1545 2229 Fragments arranged by size: 2229 1545 1530 1077 539 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7327 PciI A'CATG_T Cuts at: 4371 6226 4371 Size: 1855 5472 PflMI CCAn_nnn'nTGG Cuts at: 1108 2469 3907 4473 1108 Size: 1361 1438 566 3962 Fragments arranged by size: 3962 1438 1361 566 PfoI T'CCnGG_A Cuts at: 2104 2881 3540 2104 Size: 777 659 5891 Fragments arranged by size: 5891 777 659 PmeI GTTT'AAAC Cuts at: 4631 4631 Size: 7327 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5396 5428 6909 6941 5396 Size: 32 1481 32 5782 Fragments arranged by size: 5782 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2562 3853 4542 2562 Size: 1291 689 5347 Fragments arranged by size: 5347 1291 689 PshAI GACnn'nnGTC Cuts at: 1613 2510 4211 1613 Size: 897 1701 4729 Fragments arranged by size: 4729 1701 897 PsiI TTA'TAA Cuts at: 4897 4897 Size: 7327 PspXI vC'TCGA_Gb Cuts at: 3878 3878 Size: 7327 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3791 3823 3791 Size: 32 7295 PstI C_TGCA'G Cuts at: 830 4398 4679 830 Size: 3568 281 3478 Fragments arranged by size: 3568 3478 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7327 SalI G'TCGA_C Cuts at: 1936 4610 4669 1936 Size: 2674 59 4594 Fragments arranged by size: 4594 2674 59 SanDI GG'GwC_CC Cuts at: 2562 4542 2562 Size: 1980 5347 SbfI CC_TGCA'GG Cuts at: 4398 4679 4398 Size: 281 7046 ScaI AGT'ACT Cuts at: 5575 7287 5575 Size: 1712 5615 SexAI A'CCwGG_T Cuts at: 3690 3690 Size: 7327 SfcI C'TryA_G Cuts at: 826 1045 3976 4394 4675 5810 6491 6682 826 Size: 219 2931 418 281 1135 681 191 1471 Fragments arranged by size: 2931 1471 1135 681 418 281 219 191 SfoI GGC'GCC Cuts at: 4138 4138 Size: 7327 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7327 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7327 SmaI CCC'GGG Cuts at: 1872 4654 1872 Size: 2782 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3464 3878 5431 6332 6594 820 Size: 197 926 1521 414 1553 901 262 1553 Fragments arranged by size: 1553 1553 1521 926 901 414 262 197 SnaBI TAC'GTA Cuts at: 493 5118 493 Size: 4625 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7327 SphI G_CATG'C Cuts at: 1132 2759 3298 4685 1132 Size: 1627 539 1387 3774 Fragments arranged by size: 3774 1627 1387 539 SspI AAT'ATT Cuts at: 5 52 5251 5 Size: 47 5199 2081 Fragments arranged by size: 5199 2081 47 StuI AGG'CCT Cuts at: 1517 2477 3245 1517 Size: 960 768 5599 Fragments arranged by size: 5599 960 768 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 3141 4086 4249 4281 4467 Size: 552 939 528 609 945 163 32 186 Cuts at: 4467 4577 4766 513 Size: 110 189 3074 Fragments arranged by size: 3074 945 939 609 552 528 189 186 163 110 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3430 5354 5371 5524 5709 6154 325 Size: 1255 1850 1924 17 153 185 445 1498 Fragments arranged by size: 1924 1850 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 2730 3528 Size: 274 80 33 51 1443 468 285 798 Cuts at: 3528 4368 5573 7285 96 Size: 840 1205 1712 138 Fragments arranged by size: 1712 1443 1205 840 798 468 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 2181 2213 2563 2595 3377 3409 3678 Size: 32 49 32 350 32 782 32 269 Cuts at: 3678 3710 7287 7319 2100 Size: 32 3577 32 2108 Fragments arranged by size: 3577 2108 782 350 269 49 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3071 1316 Size: 1755 5572 XbaI T'CTAG_A Cuts at: 2357 4663 2357 Size: 2306 5021 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2499 2499 Size: 7327 XhoI C'TCGA_G Cuts at: 1943 3878 1943 Size: 1935 5392 XmaI C'CCGG_G Cuts at: 1870 4652 1870 Size: 2782 4545 XmnI GAAnn'nnTTC Cuts at: 4517 5456 4517 Size: 939 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5135 276 Size: 53 83 186 936 3601 2468 Fragments arranged by size: 3601 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI