(Linear) (Six-base) MAPSORT of: KIEE9427.seq Check: 2905 from: 1 to: 2622 KIEE9427.orf With 182 enzymes: SgfI * March 22, 2012 18:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1644 2622 Size: 1644 978 AccI GT'mk_AC Cuts at: 0 77 545 2606 2622 Size: 77 468 2061 16 Fragments arranged by size: 2061 468 77 16 Acc65I G'GTAC_C Cuts at: 0 2308 2622 Size: 2308 314 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 326 462 1527 2622 Size: 154 172 136 1065 1095 Fragments arranged by size: 1095 1065 172 154 136 AfeI AGC'GCT Cuts at: 0 1801 2622 Size: 1801 821 AflIII A'CryG_T Cuts at: 0 1474 2366 2622 Size: 1474 892 256 Fragments arranged by size: 1474 892 256 AleI CACnn'nnGTG Cuts at: 0 1267 1577 2622 Size: 1267 310 1045 Fragments arranged by size: 1267 1045 310 AlwNI CAG_nnn'CTG Cuts at: 0 275 1428 1851 2397 2622 Size: 275 1153 423 546 225 Fragments arranged by size: 1153 546 423 275 225 ApoI r'AATT_y Cuts at: 0 1179 2427 2622 Size: 1179 1248 195 Fragments arranged by size: 1248 1179 195 AvaI C'yCGr_G Cuts at: 0 1873 2622 Size: 1873 749 BamHI G'GATC_C Cuts at: 0 158 2622 Size: 158 2464 BanI G'GyrC_C Cuts at: 0 2131 2308 2622 Size: 2131 177 314 Fragments arranged by size: 2131 314 177 BanII G_rGCy'C Cuts at: 0 702 1129 1438 2622 Size: 702 427 309 1184 Fragments arranged by size: 1184 702 427 309 BbeI G_GCGC'C Cuts at: 0 2135 2622 Size: 2135 487 BbsI GAAGACnn'nnnn_ Cuts at: 0 340 755 1509 2622 Size: 340 415 754 1113 Fragments arranged by size: 1113 754 415 340 BbvCI CC'TCA_GC Cuts at: 0 913 2622 Size: 913 1709 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2175 2209 2622 Size: 2175 34 413 Fragments arranged by size: 2175 413 34 BciVI GTATCCnnnnn_n' Cuts at: 0 168 918 1092 2622 Size: 168 750 174 1530 Fragments arranged by size: 1530 750 174 168 BclI T'GATC_A Cuts at: 0 1982 2622 Size: 1982 640 BfrBI ATG'CAT Cuts at: 0 1347 2622 Size: 1347 1275 BglI GCCn_nnn'nGGC Cuts at: 0 488 2140 2622 Size: 488 1652 482 Fragments arranged by size: 1652 488 482 BlpI GC'TnA_GC Cuts at: 0 2566 2622 Size: 2566 56 Bme1580I G_kGCm'C Cuts at: 0 1885 2622 Size: 1885 737 BmrI ACTGGGnnnn_n' Cuts at: 0 917 2249 2622 Size: 917 1332 373 Fragments arranged by size: 1332 917 373 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1146 1615 2622 Size: 1146 469 1007 Fragments arranged by size: 1146 1007 469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 163 203 396 1278 1414 1687 2622 Size: 66 97 40 193 882 136 273 935 Fragments arranged by size: 935 882 273 193 136 97 66 40 Bpu10I CC'TnA_GC Cuts at: 0 562 913 2622 Size: 562 351 1709 Fragments arranged by size: 1709 562 351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1444 2622 Size: 1444 1178 BsaI GGTCTCn'nnnn_ Cuts at: 0 2243 2622 Size: 2243 379 BsaAI yAC'GTr Cuts at: 0 1477 2622 Size: 1477 1145 BsaBI GATnn'nnATC Cuts at: 0 2059 2622 Size: 2059 563 BsaHI Gr'CG_yC Cuts at: 0 74 118 2132 2622 Size: 74 44 2014 490 Fragments arranged by size: 2014 490 74 44 BsaWI w'CCGG_w Cuts at: 0 708 1549 1790 2622 Size: 708 841 241 832 Fragments arranged by size: 841 832 708 241 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 176 206 256 286 1531 1544 1561 1574 Size: 176 30 50 30 1245 13 17 13 Cuts at: 1574 2622 Size: 1048 Fragments arranged by size: 1245 1048 176 50 30 30 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1119 2622 Size: 1119 1503 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1078 1605 1948 2622 Size: 1078 527 343 674 Fragments arranged by size: 1078 674 527 343 BsiHKAI G_wGCw'C Cuts at: 0 568 1264 1736 1786 1913 2622 Size: 568 696 472 50 127 709 Fragments arranged by size: 709 696 568 472 127 50 Bsp1286I G_dGCh'C Cuts at: 0 568 702 1129 1264 1438 1736 1786 1885 Size: 568 134 427 135 174 298 50 99 Cuts at: 1885 1913 2622 Size: 28 709 Fragments arranged by size: 709 568 427 298 174 135 134 99 50 28 BspEI T'CCGG_A Cuts at: 0 708 1549 1790 2622 Size: 708 841 241 832 Fragments arranged by size: 841 832 708 241 BspHI T'CATG_A Cuts at: 0 785 2528 2622 Size: 785 1743 94 Fragments arranged by size: 1743 785 94 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 251 1100 1644 2622 Size: 251 849 544 978 Fragments arranged by size: 978 849 544 251 BsrBI CCG'CTC Cuts at: 0 696 2622 Size: 696 1926 BsrGI T'GTAC_A Cuts at: 0 440 2622 Size: 440 2182 BssSI C'ACGA_G Cuts at: 0 593 622 1234 1587 2313 2478 2622 Size: 593 29 612 353 726 165 144 Fragments arranged by size: 726 612 593 353 165 144 29 BstAPI GCAn_nnn'nTGC Cuts at: 0 2108 2622 Size: 2108 514 BstEII G'GTnAC_C Cuts at: 0 1113 1755 2622 Size: 1113 642 867 Fragments arranged by size: 1113 867 642 BstXI CCAn_nnnn'nTGG Cuts at: 0 2283 2622 Size: 2283 339 BstYI r'GATC_y Cuts at: 0 158 989 1226 2240 2622 Size: 158 831 237 1014 382 Fragments arranged by size: 1014 831 382 237 158 BtgI C'CryG_G Cuts at: 0 980 1034 1118 1136 1151 1206 1572 2081 Size: 980 54 84 18 15 55 366 509 Cuts at: 2081 2244 2622 Size: 163 378 Fragments arranged by size: 980 509 378 366 163 84 55 54 18 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2178 2622 Size: 2178 444 BtsI GCAGTG_nn' Cuts at: 0 301 731 2288 2622 Size: 301 430 1557 334 Fragments arranged by size: 1557 430 334 301 DraIII CAC_nnn'GTG Cuts at: 0 1689 2622 Size: 1689 933 EaeI y'GGCC_r Cuts at: 0 136 1154 1745 1904 2285 2622 Size: 136 1018 591 159 381 337 Fragments arranged by size: 1018 591 381 337 159 136 EarI CTCTTCn'nnn_ Cuts at: 0 787 848 2054 2622 Size: 787 61 1206 568 Fragments arranged by size: 1206 787 568 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1858 2478 2622 Size: 1858 620 144 Fragments arranged by size: 1858 620 144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 154 163 203 326 396 462 1278 Size: 66 88 9 40 123 70 66 816 Cuts at: 1278 1414 1527 1687 2622 Size: 136 113 160 935 Fragments arranged by size: 935 816 160 136 123 113 88 70 66 66 40 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 1245 1651 2622 Size: 226 1019 406 971 Fragments arranged by size: 1019 971 406 226 EcoO109I rG'GnC_Cy Cuts at: 0 490 534 557 1848 2537 2622 Size: 490 44 23 1291 689 85 Fragments arranged by size: 1291 689 490 85 44 23 EcoRI G'AATT_C Cuts at: 0 1179 2427 2622 Size: 1179 1248 195 Fragments arranged by size: 1248 1179 195 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 987 2622 Size: 987 1635 HaeII r_GCGC'y Cuts at: 0 1034 1696 1803 2135 2228 2622 Size: 1034 662 107 332 93 394 Fragments arranged by size: 1034 662 394 332 107 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 371 403 1531 1544 1563 1576 2622 Size: 371 32 1128 13 19 13 1046 Fragments arranged by size: 1128 1046 371 32 19 13 13 HincII GTy'rAC Cuts at: 0 207 552 618 2178 2270 2340 2607 2622 Size: 207 345 66 1560 92 70 267 15 Fragments arranged by size: 1560 345 267 207 92 70 66 15 KasI G'GCGC_C Cuts at: 0 2131 2622 Size: 2131 491 KpnI G_GTAC'C Cuts at: 0 2312 2622 Size: 2312 310 MfeI C'AATT_G Cuts at: 0 2106 2622 Size: 2106 516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 635 988 1112 1979 2525 2622 Size: 635 353 124 867 546 97 Fragments arranged by size: 867 635 546 353 124 97 MscI TGG'CCA Cuts at: 0 138 1156 1747 2622 Size: 138 1018 591 875 Fragments arranged by size: 1018 875 591 138 MslI CAynn'nnrTG Cuts at: 0 1069 1267 1577 1648 1654 2197 2622 Size: 1069 198 310 71 6 543 425 Fragments arranged by size: 1069 543 425 310 198 71 6 MspA1I CmG'CkG Cuts at: 0 416 1619 1665 2622 Size: 416 1203 46 957 Fragments arranged by size: 1203 957 416 46 NarI GG'CG_CC Cuts at: 0 2132 2622 Size: 2132 490 NcoI C'CATG_G Cuts at: 0 1136 2081 2244 2622 Size: 1136 945 163 378 Fragments arranged by size: 1136 945 378 163 NsiI A_TGCA'T Cuts at: 0 1349 2622 Size: 1349 1273 NspI r_CATG'y Cuts at: 0 754 1293 2370 2622 Size: 754 539 1077 252 Fragments arranged by size: 1077 754 539 252 PciI A'CATG_T Cuts at: 0 2366 2622 Size: 2366 256 PflMI CCAn_nnn'nTGG Cuts at: 0 464 1902 2468 2622 Size: 464 1438 566 154 Fragments arranged by size: 1438 566 464 154 PfoI T'CCnGG_A Cuts at: 0 99 876 1535 2622 Size: 99 777 659 1087 Fragments arranged by size: 1087 777 659 99 PpuMI rG'GwC_Cy Cuts at: 0 557 1848 2537 2622 Size: 557 1291 689 85 Fragments arranged by size: 1291 689 557 85 PshAI GACnn'nnGTC Cuts at: 0 505 2206 2622 Size: 505 1701 416 Fragments arranged by size: 1701 505 416 PspXI vC'TCGA_Gb Cuts at: 0 1873 2622 Size: 1873 749 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1786 1818 2622 Size: 1786 32 804 Fragments arranged by size: 1786 804 32 PstI C_TGCA'G Cuts at: 0 2393 2622 Size: 2393 229 SalI G'TCGA_C Cuts at: 0 2605 2622 Size: 2605 17 SanDI GG'GwC_CC Cuts at: 0 557 2537 2622 Size: 557 1980 85 Fragments arranged by size: 1980 557 85 SbfI CC_TGCA'GG Cuts at: 0 2393 2622 Size: 2393 229 SexAI A'CCwGG_T Cuts at: 0 1685 2622 Size: 1685 937 SfcI C'TryA_G Cuts at: 0 1971 2389 2622 Size: 1971 418 233 Fragments arranged by size: 1971 418 233 SfoI GGC'GCC Cuts at: 0 2133 2622 Size: 2133 489 SmlI C'TyrA_G Cuts at: 0 1459 1873 2622 Size: 1459 414 749 Fragments arranged by size: 1459 749 414 SphI G_CATG'C Cuts at: 0 754 1293 2622 Size: 754 539 1329 Fragments arranged by size: 1329 754 539 StuI AGG'CCT Cuts at: 0 472 1240 2622 Size: 472 768 1382 Fragments arranged by size: 1382 768 472 StyI C'CwwG_G Cuts at: 0 527 1136 2081 2244 2276 2462 2572 2622 Size: 527 609 945 163 32 186 110 50 Fragments arranged by size: 945 609 527 186 163 110 50 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1425 2622 Size: 1425 1197 TatI w'GTAC_w Cuts at: 0 440 725 1523 2363 2622 Size: 440 285 798 840 259 Fragments arranged by size: 840 798 440 285 259 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 95 127 176 208 558 590 1372 1404 Size: 95 32 49 32 350 32 782 32 Cuts at: 1404 1673 1705 2622 Size: 269 32 917 Fragments arranged by size: 917 782 350 269 95 49 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1066 2622 Size: 1066 1556 XbaI T'CTAG_A Cuts at: 0 352 2622 Size: 352 2270 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 494 2622 Size: 494 2128 XhoI C'TCGA_G Cuts at: 0 1873 2622 Size: 1873 749 XmnI GAAnn'nnTTC Cuts at: 0 2512 2622 Size: 2512 110 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI KpnI MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI PpuMI PshAI PspXI PsrI PstI SalI SanDI SbfI SexAI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BglII BmgBI BmtI BseYI BsiEI BsiWI BsmI BsmBI BsrDI BsrFI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PsiI PspOMI PvuI PvuII RsrII SacI SacII SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XmaI ZraI