################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AhdI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbvCI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BseRI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MaeI MaeIII MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI ScaI SfuI SnaBI SpeI SphI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1168 GACGTC GACGT^C AccI 814 GTCTAC GT^[AC][GT]AC AccIII 1268 TCCGGA T^CCGGA AflIII 647 ACGTGT A^C[GA][CT]GT Alw44I 282 GTGCAC G^TGCAC Aor51HI 671 AGCGCT AGC^GCT ApaLI 282 GTGCAC G^TGCAC AviII 1115 TGCGCA TGC^GCA BbrPI 598 CACGTG CAC^GTG BcgI 125 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1649 TGATCA T^GATCA BsaI 294 GGTCTC GGTCTC(1/5) BsaWI 1268 TCCGGA [AT]^CCGG[AT] BseAI 1268 TCCGGA T^CCGGA BsmAI 295 GTCTC GTCTC(1/5) Bsp1407I 1349 TGTACA T^GTACA BspEI 1268 TCCGGA T^CCGGA BsrBI 625 CCGCTC CCGCTC(-3/-3) BsrGI 1349 TGTACA T^GTACA DraIII 595 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1578 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 508 CGGCCG C^GGCCG EclXI 508 CGGCCG C^GGCCG Eco47III 671 AGCGCT AGC^GCT Eco52I 508 CGGCCG C^GGCCG EcoT14I 68 CCATGG C^C[AT][AT]GG FbaI 1649 TGATCA T^GATCA FspI 1115 TGCGCA TGC^GCA HincII 1171 GTCAAC GT[CT]^[GA]AC HindII 1171 GTCAAC GT[CT]^[GA]AC MroI 1268 TCCGGA T^CCGGA MseI 1615 TTAA T^TAA NcoI 68 CCATGG C^CATGG PflMI 594 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 598 CACGTG CAC^GTG PmlI 598 CACGTG CAC^GTG SacI 1135 GAGCTC GAGCT^C SbfI 33 CCTGCAGG CCTGCA^GG SfiI 509 GGCCGCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 846 CGCCGGCG C[GA]^CCGG[CT]G SmlI 343 CTCAAG C^T[CT][GA]AG Sse8387I 33 CCTGCAGG CCTGCA^GG SspBI 1349 TGTACA T^GTACA StyI 68 CCATGG C^C[AT][AT]GG Tru9I 1615 TTAA T^TAA TspRI 680 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 594 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 12 GGGCCC GGGCC^C 555 GGGCCC BalI 692 TGGCCA TGG^CCA 962 TGGCCA BanI 447 GGCGCC G^G[CT][GA]CC 850 GGCGCC BbeI 447 GGCGCC GGCGC^C 850 GGCGCC BglII 1505 AGATCT A^GATCT 1520 AGATCT BsaAI 598 CACGTG [CT]AC^GT[GA] 1153 TACGTG BsiEI 508 CGGCCG CG[GA][CT]^CG 870 CGACCG BssHII 527 GCGCGC G^CGCGC 620 GCGCGC BstAPI 860 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 917 GCACCGGCTGC BstXI 68 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 158 CCAACCTGATGG HgaI 613 GCGTC GACGC(5/10) 812 GCGTC KasI 447 GGCGCC G^GCGCC 850 GGCGCC MluNI 692 TGGCCA TGG^CCA 962 TGGCCA MscI 692 TGGCCA TGG^CCA 962 TGGCCA NarI 447 GGCGCC GG^CGCC 850 GGCGCC PstI 34 CTGCAG CTGCA^G 1582 CTGCAG SexAI 997 ACCTGGT A^CC[AT]GGT 1214 ACCTGGT SfaNI 860 GCATC GCATC(5/9) 1683 GCATC SfcI 34 CTGCAG C^T[GA][CT]AG 1582 CTGCAG SfoI 447 GGCGCC GGC^GCC 850 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI SapI Sau3AI Sau96I ScrFI SmaI TaqI TseI TthHB8I XcmI XhoII XmaCI XmaI