(Circular) (Six-base) MAPSORT of: pFN21AE2005.seq Check: 6575 from: 1 to: 6448 pFN21AE2005 6448 bp With 182 enzymes: SgfI * March 28, 2012 12:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 3592 3749 2032 Size: 1560 157 4731 Fragments arranged by size: 4731 1560 157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3177 4258 278 Size: 53 83 186 936 1641 1081 2468 Fragments arranged by size: 2468 1641 1081 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3791 1937 Size: 883 971 4594 Fragments arranged by size: 4594 971 883 Acc65I G'GTAC_C Cuts at: 3769 3769 Size: 6448 AclI AA'CG_TT Cuts at: 1563 4575 4948 1563 Size: 3012 373 3063 Fragments arranged by size: 3063 3012 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 4511 5895 6163 1632 Size: 938 1941 1384 268 1917 Fragments arranged by size: 1941 1917 1384 938 268 AfeI AGC'GCT Cuts at: 2678 2678 Size: 6448 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6251 AflIII A'CryG_T Cuts at: 1203 2652 5347 6324 1203 Size: 1449 2695 977 1327 Fragments arranged by size: 2695 1449 1327 977 AgeI A'CCGG_T Cuts at: 5279 5279 Size: 6448 AhdI GACnn_n'nnGTC Cuts at: 5177 5177 Size: 6448 AleI CACnn'nnGTG Cuts at: 1166 3374 3743 1166 Size: 2208 369 3871 Fragments arranged by size: 3871 2208 369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6448 AlwNI CAG_nnn'CTG Cuts at: 4191 5763 4191 Size: 1572 4876 ApaI G_GGCC'C Cuts at: 2021 2564 2021 Size: 543 5905 ApaLI G'TGCA_C Cuts at: 2287 4505 5661 2287 Size: 2218 1156 3074 Fragments arranged by size: 3074 2218 1156 ApoI r'AATT_y Cuts at: 1493 3757 3983 4135 6244 1493 Size: 2264 226 152 2109 1697 Fragments arranged by size: 2264 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5002 160 Size: 872 3970 1606 Fragments arranged by size: 3970 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2089 2481 2488 2564 2587 2780 Size: 73 71 75 392 7 76 23 193 Cuts at: 2780 3247 3773 1870 Size: 467 526 4545 Fragments arranged by size: 4545 526 467 392 193 76 75 73 71 23 7 BamHI G'GATC_C Cuts at: 3778 4145 3778 Size: 367 6081 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 2855 3769 5224 618 Size: 325 217 627 665 403 914 1455 1842 Fragments arranged by size: 1842 1455 914 665 627 403 325 217 BanII G_rGCy'C Cuts at: 1391 2021 2054 2478 2489 2564 3144 3342 3767 Size: 630 33 424 11 75 580 198 425 Cuts at: 3767 1391 Size: 4072 Fragments arranged by size: 4072 630 580 425 424 198 75 33 11 BbeI G_GCGC'C Cuts at: 2456 2859 2456 Size: 403 6045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2932 3104 3293 928 Size: 971 1033 172 189 4083 Fragments arranged by size: 4083 1033 971 189 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2119 2153 4639 4673 1903 Size: 34 50 34 98 34 2486 34 3678 Fragments arranged by size: 3678 2486 98 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4340 5550 4340 Size: 1210 5238 BclI T'GATC_A Cuts at: 1548 3654 1548 Size: 2106 4342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2521 2705 3089 Size: 107 122 71 631 605 849 184 384 Cuts at: 3089 5059 136 Size: 1970 1525 Fragments arranged by size: 1970 1525 849 631 605 384 184 122 107 71 BglII A'GATC_T Cuts at: 3510 3525 6443 3510 Size: 15 2918 3515 Fragments arranged by size: 3515 2918 15 BlpI GC'TnA_GC Cuts at: 3865 3865 Size: 6448 Bme1580I G_kGCm'C Cuts at: 1790 2021 2291 2564 4509 5665 1790 Size: 231 270 273 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 273 270 231 BmgBI CAC'GTC Cuts at: 1318 2653 3234 1318 Size: 1335 581 4532 Fragments arranged by size: 4532 1335 581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3724 4179 5137 Size: 416 53 83 399 24 2307 455 958 Cuts at: 5137 442 Size: 1753 Fragments arranged by size: 2307 1753 958 455 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6448 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 6448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2680 2710 2936 3335 5108 1239 Size: 1441 30 226 399 1773 2579 Fragments arranged by size: 2579 1773 1441 399 226 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 4573 5438 5736 2333 Size: 2240 865 298 3045 Fragments arranged by size: 3045 2240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2305 5111 882 Size: 617 806 2806 2219 Fragments arranged by size: 2806 2219 806 617 BsaAI yAC'GTr Cuts at: 493 1204 2605 3160 4239 493 Size: 711 1401 555 1079 2702 Fragments arranged by size: 2702 1401 1079 711 555 BsaBI GATnn'nnATC Cuts at: 6442 6442 Size: 6448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2453 2856 3174 4255 Size: 53 83 186 936 920 403 318 1081 Cuts at: 4255 4637 5272 275 Size: 382 635 1451 Fragments arranged by size: 1451 1081 936 920 635 403 382 318 186 83 53 BsaWI w'CCGG_w Cuts at: 3273 4881 5279 5553 5700 6111 3273 Size: 1608 398 274 147 411 3610 Fragments arranged by size: 3610 1608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6223 Fragments arranged by size: 6223 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6448 BseYI C'CCAG_C Cuts at: 3684 5651 6147 3684 Size: 1967 496 3985 Fragments arranged by size: 3985 1967 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2620 2914 3628 1163 Size: 573 884 294 714 3983 Fragments arranged by size: 3983 884 714 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2516 2878 3898 4659 5687 1540 Size: 275 186 515 362 1020 761 1028 2301 Fragments arranged by size: 2301 1028 1020 761 515 362 275 186 BsiHKAI G_wGCw'C Cuts at: 2270 2291 2357 2544 2745 2924 3144 3201 4509 Size: 21 66 187 201 179 220 57 1308 Cuts at: 4509 4594 5665 2270 Size: 85 1071 3053 Fragments arranged by size: 3053 1308 1071 220 201 187 179 85 66 57 21 BsmI GAATG_Cn' Cuts at: 3959 4052 3959 Size: 93 6355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2054 2270 2291 2357 2478 2489 Size: 399 231 33 216 21 66 121 11 Cuts at: 2489 2544 2564 2745 2924 3144 3201 3342 3767 Size: 55 20 181 179 220 57 141 425 Cuts at: 3767 4509 4594 5665 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 425 399 231 220 216 181 179 141 121 85 66 57 55 33 21 20 11 BspEI T'CCGG_A Cuts at: 3273 6111 3273 Size: 2838 3610 BspHI T'CATG_A Cuts at: 4337 6067 4337 Size: 1730 4718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 2287 3592 3749 3803 844 Size: 1188 255 1305 157 54 3489 Fragments arranged by size: 3489 1305 1188 255 157 54 BsrBI CCG'CTC Cuts at: 1394 1823 2632 4335 1394 Size: 429 809 1703 3507 Fragments arranged by size: 3507 1703 809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4943 5117 65 Size: 1183 3695 174 1396 Fragments arranged by size: 3695 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2852 2924 3017 3188 3364 3643 5092 Size: 110 1042 72 93 171 176 279 1449 Cuts at: 5092 5279 1700 Size: 187 2869 Fragments arranged by size: 2869 1449 1042 279 187 176 171 110 93 72 BsrGI T'GTAC_A Cuts at: 96 3354 96 Size: 3258 3190 BssHII G'CGCG_C Cuts at: 1925 2532 2625 1925 Size: 607 93 5748 Fragments arranged by size: 5748 607 93 BssSI C'ACGA_G Cuts at: 2053 2177 2210 3035 4508 5520 2053 Size: 124 33 825 1473 1012 2981 Fragments arranged by size: 2981 1473 1012 825 124 33 BstAPI GCAn_nnn'nTGC Cuts at: 794 2871 2928 794 Size: 2077 57 4314 Fragments arranged by size: 4314 2077 57 BstBI TT'CG_AA Cuts at: 5252 5252 Size: 6448 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6448 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2170 3745 2080 Size: 90 1575 4783 Fragments arranged by size: 4783 1575 90 BstYI r'GATC_y Cuts at: 1972 2118 3423 3510 3525 3720 3778 4145 4530 Size: 146 1305 87 15 195 58 367 385 Cuts at: 4530 4547 5999 6085 6097 6443 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 195 146 87 86 58 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2073 2242 2826 3504 3706 513 Size: 552 1008 169 584 678 202 3255 Fragments arranged by size: 3255 1008 678 584 552 202 169 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2851 487 Size: 683 56 1625 4084 Fragments arranged by size: 4084 1625 683 56 BtsI GCAGTG_nn' Cuts at: 2691 3964 4758 4778 2691 Size: 1273 794 20 4361 Fragments arranged by size: 4361 1273 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6413 DraI TTT'AAA Cuts at: 3752 3752 Size: 6448 DraIII CAC_nnn'GTG Cuts at: 2605 2605 Size: 6448 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3589 5455 809 Size: 507 2273 1866 1802 Fragments arranged by size: 2273 1866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2513 2697 Size: 54 1425 50 275 18 415 268 184 Cuts at: 2697 2887 2967 3012 3366 3486 3645 3895 4784 Size: 190 80 45 354 120 159 250 889 Cuts at: 4784 6413 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 415 354 275 268 250 190 184 159 120 80 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2513 3895 1537 Size: 275 701 1382 4090 Fragments arranged by size: 4090 1382 701 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 2852 3347 3623 4378 1353 Size: 1253 246 495 276 755 3423 Fragments arranged by size: 3423 1253 755 495 276 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2831 5035 5409 5555 387 Size: 1064 138 1242 2204 374 146 1280 Fragments arranged by size: 2204 1280 1242 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3142 3142 Size: 6448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2570 2680 2710 2936 3335 4511 5108 Size: 393 938 110 30 226 399 1176 597 Cuts at: 5108 5895 6163 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1176 938 787 597 399 393 268 226 110 30 EcoO109I rG'GnC_Cy Cuts at: 2018 2560 2561 2018 Size: 542 1 5905 Fragments arranged by size: 5905 542 1 EcoRI G'AATT_C Cuts at: 3757 4135 3757 Size: 378 6070 FspI TGC'GCA Cuts at: 3122 4954 3122 Size: 1832 4616 HaeII r_GCGC'y Cuts at: 1678 2014 2089 2456 2680 2733 2788 2859 5595 Size: 336 75 367 224 53 55 71 2736 Cuts at: 5595 1678 Size: 2531 Fragments arranged by size: 2736 2531 367 336 224 75 71 55 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5089 5121 5163 5195 1373 Size: 32 135 32 3517 32 42 32 2626 Fragments arranged by size: 3517 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3792 4038 669 Size: 988 281 1240 614 246 3079 Fragments arranged by size: 3079 1240 988 614 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6448 HpaI GTT'AAC Cuts at: 4038 4038 Size: 6448 KasI G'GCGC_C Cuts at: 2452 2855 2452 Size: 403 6045 KpnI G_GTAC'C Cuts at: 3773 3773 Size: 6448 MfeI C'AATT_G Cuts at: 4047 4047 Size: 6448 MluI A'CGCG_T Cuts at: 6324 6324 Size: 6448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5562 5746 1305 Size: 4257 184 2007 Fragments arranged by size: 4257 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2699 2969 10 Size: 54 1425 343 867 270 3489 Fragments arranged by size: 3489 1425 867 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 3374 3743 4406 4765 4924 518 Size: 648 912 1296 369 663 359 159 2042 Fragments arranged by size: 2042 1296 912 663 648 369 359 159 MspA1I CmG'CkG Cuts at: 1606 2224 2506 2521 2548 2886 3558 3663 3864 Size: 618 282 15 27 338 672 105 201 Cuts at: 3864 4541 5689 5934 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 618 338 282 245 201 105 27 15 NaeI GCC'GGC Cuts at: 2854 3019 3366 2854 Size: 165 347 5936 Fragments arranged by size: 5936 347 165 NarI GG'CG_CC Cuts at: 2453 2856 2453 Size: 403 6045 NcoI C'CATG_G Cuts at: 513 1065 2073 513 Size: 552 1008 4888 Fragments arranged by size: 4888 1008 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6448 NgoMIV G'CCGG_C Cuts at: 2852 3017 3364 2852 Size: 165 347 5936 Fragments arranged by size: 5936 347 165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6448 NotI GC'GGCC_GC Cuts at: 3895 3895 Size: 6448 NspI r_CATG'y Cuts at: 1132 1229 3806 5351 1132 Size: 97 2577 1545 2229 Fragments arranged by size: 2577 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6448 PciI A'CATG_T Cuts at: 5347 5347 Size: 6448 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 4951 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 6448 PmeI GTTT'AAAC Cuts at: 3752 3752 Size: 6448 PmlI CAC'GTG Cuts at: 2605 2605 Size: 6448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4517 4549 6030 6062 4517 Size: 32 1481 32 4903 Fragments arranged by size: 4903 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6448 PsiI TTA'TAA Cuts at: 4018 4018 Size: 6448 PspOMI G'GGCC_C Cuts at: 2017 2560 2017 Size: 543 5905 PstI C_TGCA'G Cuts at: 830 2043 3591 3800 830 Size: 1213 1548 209 3478 Fragments arranged by size: 3478 1548 1213 209 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6448 SacI G_AGCT'C Cuts at: 3144 3144 Size: 6448 SalI G'TCGA_C Cuts at: 1936 3790 1936 Size: 1854 4594 SapI GCTCTTCn'nnn_ Cuts at: 2606 3347 3623 2606 Size: 741 276 5431 Fragments arranged by size: 5431 741 276 SbfI CC_TGCA'GG Cuts at: 2043 3800 2043 Size: 1757 4691 ScaI AGT'ACT Cuts at: 4696 6408 4696 Size: 1712 4736 SexAI A'CCwGG_T Cuts at: 3002 3219 3002 Size: 217 6231 SfcI C'TryA_G Cuts at: 826 1045 2039 3587 3796 4931 5612 5803 826 Size: 219 994 1548 209 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 994 681 219 209 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2521 2521 Size: 6448 SfoI GGC'GCC Cuts at: 2454 2857 2454 Size: 403 6045 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 SgrAI Cr'CCGG_yG Cuts at: 1700 2852 1700 Size: 1152 5296 SmaI CCC'GGG Cuts at: 1872 2016 2091 2483 2566 2589 3775 1872 Size: 144 75 392 83 23 1186 4545 Fragments arranged by size: 4545 1186 392 144 83 75 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 4552 5453 5715 820 Size: 197 926 405 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 4239 493 Size: 3746 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6448 SphI G_CATG'C Cuts at: 1132 3806 1132 Size: 2674 3774 SrfI GCCC'GGGC Cuts at: 2016 2091 2016 Size: 75 6373 SspI AAT'ATT Cuts at: 5 52 4372 5 Size: 47 4320 2081 Fragments arranged by size: 4320 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6448 StyI C'CwwG_G Cuts at: 513 1065 2073 3887 513 Size: 552 1008 1814 3074 Fragments arranged by size: 3074 1814 1008 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2974 4475 4492 4645 4830 5275 325 Size: 1255 1394 1501 17 153 185 445 1498 Fragments arranged by size: 1501 1498 1394 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 3354 4694 Size: 274 80 33 51 1443 678 699 1340 Cuts at: 4694 6406 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1340 699 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6408 6440 6408 Size: 32 6416 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6448 XbaI T'CTAG_A Cuts at: 3784 3784 Size: 6448 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 3109 3502 2071 Size: 1038 393 5017 Fragments arranged by size: 5017 1038 393 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6448 XmaI C'CCGG_G Cuts at: 1870 2014 2089 2481 2564 2587 3773 1870 Size: 144 75 392 83 23 1186 4545 Fragments arranged by size: 4545 1186 392 144 83 75 23 XmnI GAAnn'nnTTC Cuts at: 4577 4577 Size: 6448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3175 4256 276 Size: 53 83 186 936 1641 1081 2468 Fragments arranged by size: 2468 1641 1081 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SwaI