################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BfaI BfrI BglI BlnI BpuAI BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 367 GTAGAC GT^[AC][GT]AC AcsI 522 AAATTT [GA]^AATT[CT] ApaI 1074 GGGCCC GGGCC^C ApoI 522 AAATTT [GA]^AATT[CT] AspHI 988 GAGCTC G[AT]GC[AT]^C BamHI 752 GGATCC G^GATCC BanI 8 GGCACC G^G[CT][GA]CC BcgI 977 GCACGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 381 CCGGG CC^[GC]GG BglII 413 AGATCT A^GATCT BlpI 620 GCTCAGC GC^T[ACGT]AGC Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC BsaWI 1045 TCCGGT [AT]^CCGG[AT] BsiHKAI 988 GAGCTC G[AT]GC[AT]^C BsmAI 651 GAGAC GTCTC(1/5) BsrBI 807 CCGCTC CCGCTC(-3/-3) BsrDI 131 GCAATG GCAATG(2/0) BssSI 978 CACGAG CACGAG(-5/-1) BstAPI 9 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 620 GCTCAGC GC^T[ACGT]AGC CfoI 1019 GCGC GCG^C DraII 998 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 70 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 310 CTCTTC CTCTTC(1/4) EcoO109I 998 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 1054 CCATGG C^C[AT][AT]GG HaeII 1018 GGCGCT [GA]GCGC^[CT] HgaI 983 GCGTC GACGC(5/10) HhaI 1019 GCGC GCG^C HinP1I 1019 GCGC G^CGC HinfI 3 GAATC G^A[ACGT]TC Ksp632I 310 CTCTTC CTCTTC(1/4) MseI 1033 TTAA T^TAA MspA1I 930 CCGCTG C[AC]G^C[GT]G NaeI 299 GCCGGC GCC^GGC NciI 381 CCGGG CC^[GC]GG NcoI 1054 CCATGG C^CATGG NgoMIV 299 GCCGGC G^CCGGC PpuMI 998 GGGACCC [GA]G^G[AT]CC[CT] PstI 16 CTGCAG CTGCA^G SacI 988 GAGCTC GAGCT^C SapI 309 GCTCTTC GCTCTTC(1/4) SfcI 16 CTGCAG C^T[GA][CT]AG SgrAI 92 CGCCGGTG C[GA]^CCGG[CT]G SmlI 166 CTCAAG C^T[CT][GA]AG StyI 1054 CCATGG C^C[AT][AT]GG TaqI 986 TCGA T^CGA TfiI 3 GAATC G^A[AT]TC Tru9I 1033 TTAA T^TAA TthHB8I 986 TCGA T^CGA XcmI 572 CCATGTTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 804 GTAC GT^AC 879 GTAC AlwNI 209 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 540 CAGCATCTG BalI 707 TGGCCA TGG^CCA 1037 TGGCCA BclI 227 TGATCA T^GATCA 923 TGATCA BsaBI 498 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 753 GATCCGCATC BsgI 772 CTGCAC GTGCAG(16/14) 528 GTGCAG BsrI 275 ACTGG ACTGG(1/-1) 558 CCAGT BstYI 413 AGATCT [GA]^GATC[CT] 752 GGATCC DdeI 621 CTCAG C^T[ACGT]AG 838 CTCAG DsaI 872 CCGTGG C^C[GA][CT]GG 1054 CCATGG EaeI 707 TGGCCA [CT]^GGCC[GA] 1037 TGGCCA FbaI 227 TGATCA T^GATCA 923 TGATCA MamI 498 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 753 GATCCGCATC MflI 413 AGATCT [GA]^GATC[CT] 752 GGATCC MluNI 707 TGGCCA TGG^CCA 1037 TGGCCA MscI 707 TGGCCA TGG^CCA 1037 TGGCCA NspI 666 GCATGC [GA]CATG^[CT] 860 GCATGC RsaI 804 GTAC GT^AC 879 GTAC SphI 666 GCATGC GCATG^C 860 GCATGC TspRI 209 CAGTG CA[GC]TG[ACGT][ACGT]^ 559 CAGTG XhoII 413 AGATCT [GA]^GATC[CT] 752 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I