(Circular) (Six-base) MAPSORT of: pFN21AE0806.seq Check: 9455 from: 1 to: 5794 pFN21AE0806 5794 bp With 182 enzymes: SgfI * March 28, 2012 12:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3137 1937 Size: 436 764 4594 Fragments arranged by size: 4594 764 436 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 2527 3857 5241 5509 1632 Size: 881 14 1330 1384 268 1917 Fragments arranged by size: 1917 1384 1330 881 268 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 4597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2548 1184 Size: 1364 4430 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 2813 2845 2405 Size: 32 376 32 5354 Fragments arranged by size: 5354 376 32 32 AlwNI CAG_nnn'CTG Cuts at: 2219 2550 3537 5109 2219 Size: 331 987 1572 2904 Fragments arranged by size: 2904 1572 987 331 ApaI G_GGCC'C Cuts at: 3083 3083 Size: 5794 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2527 3103 3329 3481 5590 1493 Size: 1034 576 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 576 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 3119 1870 Size: 73 1176 4545 Fragments arranged by size: 4545 1176 73 BamHI G'GATC_C Cuts at: 2757 3124 3491 2757 Size: 367 367 5060 Fragments arranged by size: 5060 367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 3115 4570 618 Size: 325 217 627 226 1102 1455 1842 Fragments arranged by size: 1842 1455 1102 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 2997 3068 3083 3113 1391 Size: 1606 71 15 30 4072 Fragments arranged by size: 4072 1606 71 30 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2971 3005 3985 4019 1903 Size: 34 1034 34 980 34 3678 Fragments arranged by size: 3678 1034 980 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 2232 2928 1548 Size: 684 696 4414 Fragments arranged by size: 4414 696 684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4405 136 Size: 107 122 71 631 605 2733 1525 Fragments arranged by size: 2733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2418 5789 2418 Size: 3371 2423 BlpI GC'TnA_GC Cuts at: 2626 3211 2626 Size: 585 5209 Bme1580I G_kGCm'C Cuts at: 1790 2792 3083 3855 5011 1790 Size: 1002 291 772 1156 2573 Fragments arranged by size: 2573 1156 1002 772 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 4483 442 Size: 416 53 83 399 24 2108 958 1753 Fragments arranged by size: 2108 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2609 2662 2878 2927 3012 4454 1239 Size: 893 477 53 216 49 85 1442 2579 Fragments arranged by size: 2579 1442 893 477 216 85 53 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 3919 4784 5082 2156 Size: 1763 865 298 2868 Fragments arranged by size: 2868 1763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3585 493 Size: 711 2381 2702 Fragments arranged by size: 2702 2381 711 BsaBI GATnn'nnATC Cuts at: 2507 2762 5788 2507 Size: 255 3026 2513 Fragments arranged by size: 3026 2513 255 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3601 3983 4618 275 Size: 53 83 186 936 2068 382 635 1451 Fragments arranged by size: 2068 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3050 4227 4625 4899 5046 5457 3050 Size: 1177 398 274 147 411 3387 Fragments arranged by size: 3387 1177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 2876 2903 2906 Size: 30 165 30 1002 30 441 27 3 Cuts at: 2906 2933 1178 Size: 27 4039 Fragments arranged by size: 4039 1002 441 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5794 BseYI C'CCAG_C Cuts at: 2741 4997 5493 2741 Size: 2256 496 3042 Fragments arranged by size: 3042 2256 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 2762 1163 Size: 573 818 208 4195 Fragments arranged by size: 4195 818 573 208 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3244 4005 5033 1540 Size: 275 186 1243 761 1028 2301 Fragments arranged by size: 2301 1243 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 3855 3940 5011 2997 Size: 858 85 1071 3780 Fragments arranged by size: 3780 1071 858 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2792 2997 3068 3083 3113 3855 3940 Size: 399 1002 205 71 15 30 742 85 Cuts at: 3940 5011 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1002 742 399 205 85 71 30 15 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 3683 5413 3683 Size: 1730 4064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3149 844 Size: 2305 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2814 3681 1394 Size: 429 991 867 3507 Fragments arranged by size: 3507 991 867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2143 4289 4463 65 Size: 1183 895 2146 174 1396 Fragments arranged by size: 2146 1396 1183 895 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2304 2667 4438 4625 1700 Size: 110 288 206 363 1771 187 2869 Fragments arranged by size: 2869 1771 363 288 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 2983 3854 4866 2983 Size: 871 1012 3911 Fragments arranged by size: 3911 1012 871 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 794 Size: 1226 4568 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstYI r'GATC_y Cuts at: 1972 2418 2757 3124 3491 3876 3893 5345 5431 Size: 446 339 367 367 385 17 1452 86 Cuts at: 5431 5443 5789 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 446 385 367 367 346 339 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2877 3059 513 Size: 552 1812 182 3248 Fragments arranged by size: 3248 1812 552 182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2220 3310 4104 4124 2220 Size: 1090 794 20 3890 Fragments arranged by size: 3890 1090 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2081 4801 809 Size: 507 765 2720 1802 Fragments arranged by size: 2720 1802 765 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 3042 3241 Size: 54 1425 50 275 18 882 330 199 Cuts at: 3241 4130 5759 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 882 330 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3241 1537 Size: 275 1429 4090 Fragments arranged by size: 4090 1429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 3724 1353 Size: 968 1403 3423 Fragments arranged by size: 3423 1403 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4381 4755 4901 387 Size: 1064 138 2792 374 146 1280 Fragments arranged by size: 2792 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 2995 Size: 5794 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2513 2527 2609 2662 2878 2927 Size: 393 500 381 14 82 53 216 49 Cuts at: 2927 3012 3857 4454 5241 5509 1239 Size: 85 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 597 500 393 381 268 216 85 82 53 49 14 EcoO109I rG'GnC_Cy Cuts at: 3004 3004 Size: 5794 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 3027 4941 1678 Size: 1349 1914 2531 Fragments arranged by size: 2531 1914 1349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 4435 4467 4509 Size: 32 135 32 838 32 1993 32 42 Cuts at: 4509 4541 1373 Size: 32 2626 Fragments arranged by size: 2626 1993 838 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3138 3384 669 Size: 988 281 1200 246 3079 Fragments arranged by size: 3079 1200 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4908 5092 1305 Size: 3603 184 2007 Fragments arranged by size: 3603 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 3044 10 Size: 54 1425 343 882 330 2760 Fragments arranged by size: 2760 1425 882 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2705 2708 2855 3752 4111 4270 Size: 648 1197 342 3 147 897 359 159 Cuts at: 4270 518 Size: 2042 Fragments arranged by size: 2042 1197 897 648 359 342 159 147 3 MspA1I CmG'CkG Cuts at: 1606 2937 3210 3887 5035 5280 1606 Size: 1331 273 677 1148 245 2120 Fragments arranged by size: 2120 1331 1148 677 273 245 NaeI GCC'GGC Cuts at: 2306 2306 Size: 5794 NcoI C'CATG_G Cuts at: 513 1065 3059 513 Size: 552 1994 3248 Fragments arranged by size: 3248 1994 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 2304 2304 Size: 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 2675 2869 3152 4697 1132 Size: 97 1446 194 283 1545 2229 Fragments arranged by size: 2229 1545 1446 283 194 97 PasI CC'CwG_GG Cuts at: 1348 2605 1348 Size: 1257 4537 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5794 PfoI T'CCnGG_A Cuts at: 2177 2586 2177 Size: 409 5385 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2405 2437 3863 3895 5376 5408 2405 Size: 32 1426 32 1481 32 2791 Fragments arranged by size: 2791 1481 1426 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3004 Size: 5794 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 3079 3079 Size: 5794 PstI C_TGCA'G Cuts at: 830 2025 3146 830 Size: 1195 1121 3478 Fragments arranged by size: 3478 1195 1121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 SacI G_AGCT'C Cuts at: 2997 2997 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SanDI GG'GwC_CC Cuts at: 3004 3004 Size: 5794 SapI GCTCTTCn'nnn_ Cuts at: 2321 2321 Size: 5794 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2021 3142 4277 4958 5149 826 Size: 219 976 1121 1135 681 191 1471 Fragments arranged by size: 1471 1135 1121 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 2098 1700 Size: 398 5396 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 3898 4799 5061 820 Size: 197 926 228 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 262 228 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 2675 2869 3152 1132 Size: 1543 194 283 3774 Fragments arranged by size: 3774 1543 283 194 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5794 StyI C'CwwG_G Cuts at: 513 1065 3059 3233 513 Size: 552 1994 174 3074 Fragments arranged by size: 3074 1994 552 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3821 3838 3991 4176 4621 325 Size: 1255 2241 17 153 185 445 1498 Fragments arranged by size: 2241 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 4040 5752 Size: 274 80 33 51 1443 906 1157 1712 Cuts at: 5752 96 Size: 138 Fragments arranged by size: 1712 1443 1157 906 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 5754 5786 2159 Size: 32 3563 32 2167 Fragments arranged by size: 3563 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2584 2584 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI