################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI DraI DrdI Eam1105I EarI EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I TspRI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 364 CGCG CG^CG AlwNI 469 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 580 AGCGCT AGC^GCT ApaI 14 GGGCCC GGGCC^C AspI 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 40 GGTCC G^G[AT]CC BamHI 368 GGATCC G^GATCC BpmI 448 CTGGAG CTGGAG(16/14) BsaBI 63 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 221 CGGCCG CG[GA][CT]^CG Bsp1407I 113 TGTACA T^GTACA BspHI 299 TCATGA T^CATGA BsrGI 113 TGTACA T^GTACA BssSI 277 CTCGTG CACGAG(-5/-1) BstEII 198 GGTGACC G^GT[ACGT]ACC BstF5I 3 GGATG GGATG(2/0) BstPI 198 GGTGACC G^GT[ACGT]ACC BstUI 364 CGCG CG^CG CpoI 39 CGGTCCG CG^G[AT]CCG DraIII 274 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 221 CGGCCG C^GGCCG EclXI 221 CGGCCG C^GGCCG Eco47III 580 AGCGCT AGC^GCT Eco52I 221 CGGCCG C^GGCCG EcoNI 402 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 198 GGTGACC G^GT[ACGT]ACC FauI 365 GCGGG CCCGC(4/6) FokI 3 GGATG GGATG(9/13) HinfI 303 GAATC G^A[ACGT]TC KspI 363 CCGCGG CCGC^GG MamI 63 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 459 CACCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 364 CGCG CG^CG NaeI 614 GCCGGC GCC^GGC NcoI 374 CCATGG C^CATGG NgoMIV 614 GCCGGC G^CCGGC NspI 464 ACATGC [GA]CATG^[CT] PflFI 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 299 TCATGA T^CATGA RsrII 39 CGGTCCG CG^G[AT]CCG SacII 363 CCGCGG CCGC^GG SfaNI 4 GATGC GCATC(5/9) SmaI 10 CCCGGG CCC^GGG SspBI 113 TGTACA T^GTACA TaqI 158 TCGA T^CGA TfiI 303 GAATC G^A[AT]TC Tth111I 319 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 158 TCGA T^CGA XmaCI 10 CCCGGG C^CCGGG XmaI 10 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 295 GACGTC GACGT^C 547 GACGTC AcyI 295 GACGTC G[GA]^CG[CT]C 547 GACGTC AfaI 114 GTAC GT^AC 165 GTAC AocI 343 CCTGAGG CC^T[ACGT]AGG 402 CCTGAGG AvaI 10 CCCGGG C^[CT]CG[GA]G 133 CCCGAG BbsI 232 GAAGAC GAAGAC(2/6) 550 GTCTTC BglI 7 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 565 GCCAAGGTGGC BpuAI 232 GAAGAC GAAGAC(2/6) 550 GTCTTC BsaHI 295 GACGTC G[GA]^CG[CT]C 547 GACGTC BsoBI 10 CCCGGG C^[CT]CG[GA]G 133 CCCGAG BsrFI 242 ACCGGC [GA]^CCGG[CT] 614 GCCGGC BstYI 368 GGATCC [GA]^GATC[CT] 491 AGATCC Bsu36I 343 CCTGAGG CC^T[ACGT]AGG 402 CCTGAGG Cfr10I 242 ACCGGC [GA]^CCGG[CT] 614 GCCGGC DsaI 363 CCGCGG C^C[GA][CT]GG 374 CCATGG EaeI 170 CGGCCA [CT]^GGCC[GA] 221 CGGCCG Eco81I 343 CCTGAGG CC^T[ACGT]AGG 402 CCTGAGG EcoT14I 374 CCATGG C^C[AT][AT]GG 566 CCAAGG Hin1I 295 GACGTC G[GA]^CG[CT]C 547 GACGTC HphI 198 GGTGA GGTGA(8/7) 458 TCACC MflI 368 GGATCC [GA]^GATC[CT] 491 AGATCC PvuII 421 CAGCTG CAG^CTG 445 CAGCTG RsaI 114 GTAC GT^AC 165 GTAC StyI 374 CCATGG C^C[AT][AT]GG 566 CCAAGG XhoII 368 GGATCC [GA]^GATC[CT] 491 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AlwI AspHI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I