################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 175 GAATTC [GA]^AATT[CT] AgeI 764 ACCGGT A^CCGGT Aor51HI 593 AGCGCT AGC^GCT ApoI 175 GAATTC [GA]^AATT[CT] AvaI 72 CTCGGG C^[CT]CG[GA]G BalI 45 TGGCCA TGG^CCA BbsI 124 GAAGAC GAAGAC(2/6) BbvI 189 GCAGC GCAGC(8/12) BcnI 59 CCCGG CC^[GC]GG BglI 431 GCCAGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 750 GCTCAGC GC^T[ACGT]AGC Bpu1102I 750 GCTCAGC GC^T[ACGT]AGC BpuAI 124 GAAGAC GAAGAC(2/6) BsaWI 764 ACCGGT [AT]^CCGG[AT] BseRI 628 CTCCTC GAGGAG(10/8) BsoBI 72 CTCGGG C^[CT]CG[GA]G BsrBI 34 GAGCGG CCGCTC(-3/-3) BsrFI 764 ACCGGT [GA]^CCGG[CT] BssHII 21 GCGCGC G^CGCGC CelII 750 GCTCAGC GC^T[ACGT]AGC Cfr10I 764 ACCGGT [GA]^CCGG[CT] Eco47III 593 AGCGCT AGC^GCT EcoNI 642 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 175 GAATTC G^AATTC EcoT14I 83 CCAAGG C^C[AT][AT]GG FauI 220 CCCGC CCCGC(4/6) HaeII 593 AGCGCT [GA]GCGC^[CT] HincII 445 GTTAAC GT[CT]^[GA]AC HindII 445 GTTAAC GT[CT]^[GA]AC HinfI 505 GATTC G^A[ACGT]TC HpaI 445 GTTAAC GTT^AAC MluNI 45 TGGCCA TGG^CCA MscI 45 TGGCCA TGG^CCA MslI 456 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 59 CCCGG CC^[GC]GG PinAI 764 ACCGGT A^CCGGT SapI 589 GAAGAGC GCTCTTC(1/4) StyI 83 CCAAGG C^C[AT][AT]GG TaqI 246 TCGA T^CGA TfiI 505 GATTC G^A[AT]TC TseI 189 GCAGC G^C[AT]GC Tsp45I 784 GTCAC ^GT[GC]AC Tsp509I 176 AATT ^AATT TthHB8I 246 TCGA T^CGA XcmI 101 CCAAGATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 54 GACGCC G[GA]^CG[CT]C 127 GACGCC AspHI 516 GTGCTC G[AT]GC[AT]^C 748 GTGCTC AvaII 284 GGTCC G^G[AT]CC 553 GGACC BsaHI 54 GACGCC G[GA]^CG[CT]C 127 GACGCC BsiHKAI 516 GTGCTC G[AT]GC[AT]^C 748 GTGCTC BsmFI 65 GGGAC GGGAC(10/14) 92 GGGAC BsrI 296 ACTGG ACTGG(1/-1) 539 CCAGT BstYI 104 AGATCC [GA]^GATC[CT] 348 GGATCT Cac8I 21 GCGCGC GC[ACGT]^[ACGT]GC 431 GCCAGC DraII 283 GGGTCCT [GA]G^G[ACGT]CC[CT] 552 AGGACCC EaeI 45 TGGCCA [CT]^GGCC[GA] 232 CGGCCA EarI 136 CTCTTC CTCTTC(1/4) 589 GAAGAG EcoO109I 283 GGGTCCT [GA]G^G[ACGT]CC[CT] 552 AGGACCC Hin1I 54 GACGCC G[GA]^CG[CT]C 127 GACGCC HphI 314 TCACC GGTGA(8/7) 785 TCACC Ksp632I 136 CTCTTC CTCTTC(1/4) 589 GAAGAG MaeIII 424 GTAAC ^GT[ACGT]AC 784 GTCAC MflI 104 AGATCC [GA]^GATC[CT] 348 GGATCT MseI 446 TTAA T^TAA 496 TTAA PpuMI 283 GGGTCCT [GA]G^G[AT]CC[CT] 552 AGGACCC SfaNI 490 GATGC GCATC(5/9) 366 GCATC Tru9I 446 TTAA T^TAA 496 TTAA TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^ 394 CAGTG XhoII 104 AGATCC [GA]^GATC[CT] 348 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanI BanII BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI