(Circular) (Six-base) MAPSORT of: pFN21AB5924.seq Check: 4982 from: 1 to: 6265 pFN21AB5924 6265 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3003 3608 1937 Size: 784 282 605 4594 Fragments arranged by size: 4594 784 605 282 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2835 3222 3450 4328 5712 5980 1632 Size: 1203 387 228 878 1384 268 1917 Fragments arranged by size: 1917 1384 1203 878 387 268 228 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2186 3247 5164 6141 1203 Size: 983 1061 1917 977 1327 Fragments arranged by size: 1917 1327 1061 983 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 2539 4994 2539 Size: 2455 3810 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3291 1184 Size: 2107 4158 AlwNI CAG_nnn'CTG Cuts at: 2278 3428 4008 5580 2278 Size: 1150 580 1572 2963 Fragments arranged by size: 2963 1572 1150 580 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2105 2468 3043 3149 3281 3574 3800 3952 Size: 612 363 575 106 132 293 226 152 Cuts at: 3952 6061 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 612 575 363 293 226 152 132 106 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3126 3590 1870 Size: 73 1183 464 4545 Fragments arranged by size: 4545 1183 464 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3586 5041 618 Size: 325 217 627 1799 1455 1842 Fragments arranged by size: 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2562 3133 3584 1391 Size: 1171 571 451 4072 Fragments arranged by size: 4072 1171 571 451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2823 928 Size: 971 924 4370 Fragments arranged by size: 4370 971 924 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2690 2927 4876 Size: 107 122 71 631 605 1018 237 1949 Cuts at: 4876 136 Size: 1525 Fragments arranged by size: 1949 1525 1018 631 605 237 122 107 71 BglII A'GATC_T Cuts at: 2586 6260 2586 Size: 3674 2591 BlpI GC'TnA_GC Cuts at: 2405 3682 2405 Size: 1277 4988 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2333 2946 3996 Size: 416 53 83 399 24 916 613 1050 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1050 958 916 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2769 3568 2769 Size: 799 5466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2209 2546 3081 3402 4925 1239 Size: 970 337 535 321 1523 2579 Fragments arranged by size: 2579 1523 970 535 337 321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3539 4928 882 Size: 617 2040 1389 2219 Fragments arranged by size: 2219 2040 1389 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 2754 6259 2754 Size: 3505 2760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2463 4698 5096 5370 5517 5928 2463 Size: 2235 398 274 147 411 2800 Fragments arranged by size: 2800 2235 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6040 Fragments arranged by size: 6040 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2646 3405 3473 1186 Size: 841 619 759 68 3978 Fragments arranged by size: 3978 841 759 619 68 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5692 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2562 4326 4411 5482 2562 Size: 1764 85 1071 3345 Fragments arranged by size: 3345 1764 1071 85 BsmI GAATG_Cn' Cuts at: 2217 3349 3776 3869 2217 Size: 1132 427 93 4613 Fragments arranged by size: 4613 1132 427 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2562 3133 3584 4326 4411 5482 1391 Size: 399 772 571 451 742 85 1071 2174 Fragments arranged by size: 2174 1071 772 742 571 451 399 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 3240 4154 5884 3240 Size: 914 1730 3621 Fragments arranged by size: 3621 1730 914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2062 4152 1394 Size: 429 239 2090 3507 Fragments arranged by size: 3507 2090 429 239 BsrDI GCAATG_nn' Cuts at: 65 1248 2848 2869 4760 4934 65 Size: 1183 1600 21 1891 174 1396 Fragments arranged by size: 1891 1600 1396 1183 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 4909 5096 1700 Size: 110 229 2870 187 2869 Fragments arranged by size: 2870 2869 229 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 2017 1925 Size: 92 6173 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2278 794 Size: 1484 4781 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2586 3595 3962 4347 4364 5816 5902 5914 Size: 614 1009 367 385 17 1452 86 12 Cuts at: 5914 6260 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1009 614 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3004 3004 Size: 6265 Bsu36I CC'TnA_GG Cuts at: 2069 2667 2069 Size: 598 5667 BtgI C'CryG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3781 Fragments arranged by size: 3781 993 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 2158 3569 2158 Size: 1411 4854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3260 5272 809 Size: 507 1944 2012 1802 Fragments arranged by size: 2012 1944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 3363 3712 Size: 54 1425 50 275 18 804 729 349 Cuts at: 3712 4601 6230 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 804 729 349 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 2711 2823 3050 3382 3537 4195 1353 Size: 1160 198 112 227 332 155 658 3423 Fragments arranged by size: 3423 1160 658 332 227 198 155 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2560 2560 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2209 2546 2835 3081 3222 3402 3450 Size: 393 577 337 289 246 141 180 48 Cuts at: 3450 4328 4925 5712 5980 1239 Size: 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 597 577 393 337 289 268 246 180 141 48 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2168 Size: 6265 EcoO109I rG'GnC_Cy Cuts at: 2065 2692 3370 2065 Size: 627 678 4960 Fragments arranged by size: 4960 678 627 EcoRI G'AATT_C Cuts at: 3281 3574 3952 3281 Size: 293 378 5594 Fragments arranged by size: 5594 378 293 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 2525 2557 2615 Size: 32 135 32 740 32 181 32 58 Cuts at: 2615 2643 2647 2675 4906 4938 4980 5012 1373 Size: 28 4 28 2231 32 42 32 2626 Fragments arranged by size: 2626 2231 740 181 135 58 42 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2851 2904 3253 3609 3855 669 Size: 988 281 913 53 349 356 246 3079 Fragments arranged by size: 3079 988 913 356 349 281 246 53 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 2904 3855 2904 Size: 951 5314 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 2546 3864 2546 Size: 1318 4947 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2708 2834 5379 5563 1305 Size: 1403 126 2545 184 2007 Fragments arranged by size: 2545 2007 1403 184 126 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2359 2841 4223 4582 4741 518 Size: 648 1193 482 1382 359 159 2042 Fragments arranged by size: 2042 1382 1193 648 482 359 159 MspA1I CmG'CkG Cuts at: 1606 2575 2777 2966 3681 4358 5506 5751 1606 Size: 969 202 189 715 677 1148 245 2120 Fragments arranged by size: 2120 1148 969 715 677 245 202 189 NaeI GCC'GGC Cuts at: 2041 2041 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3781 Fragments arranged by size: 3781 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2039 2039 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2190 3251 3623 5168 1132 Size: 97 961 1061 372 1545 2229 Fragments arranged by size: 2229 1545 1061 961 372 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 2186 3247 5164 2186 Size: 1061 1917 3287 Fragments arranged by size: 3287 1917 1061 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 6265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 2168 3835 2168 Size: 1667 4598 PstI C_TGCA'G Cuts at: 830 3352 3617 830 Size: 2522 265 3478 Fragments arranged by size: 3478 2522 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2777 2966 2777 Size: 189 6076 SacI G_AGCT'C Cuts at: 2562 2562 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 2823 2513 Size: 310 5955 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 2211 3530 4513 6225 2211 Size: 1319 983 1712 2251 Fragments arranged by size: 2251 1712 1319 983 SfcI C'TryA_G Cuts at: 826 1045 3079 3348 3613 4748 5429 5620 826 Size: 219 2034 269 265 1135 681 191 1471 Fragments arranged by size: 2034 1471 1135 681 269 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4369 5270 5532 820 Size: 197 926 2426 901 262 1553 Fragments arranged by size: 2426 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2004 2997 3256 3704 513 Size: 552 939 993 259 448 3074 Fragments arranged by size: 3074 993 939 552 448 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2633 4292 4309 4462 4647 5092 325 Size: 1255 1053 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 1053 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2209 3528 4511 Size: 274 80 33 51 1443 232 1319 983 Cuts at: 4511 6223 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1319 983 274 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6225 6257 6225 Size: 32 6233 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 2938 3601 2938 Size: 663 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3373 3373 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI