################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvrII BbeI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwNI 36 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 9 AGCGCT AGC^GCT AspI 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 686 TGCGCA TGC^GCA BalI 56 TGGCCA TGG^CCA BamHI 70 GGATCC G^GATCC BanI 637 GGCACC G^G[CT][GA]CC BbrPI 261 CACGTG CAC^GTG BbsI 483 GAAGAC GAAGAC(2/6) BfaI 574 CTAG C^TAG BglI 454 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 205 CTGGAG CTGGAG(16/14) BpuAI 483 GAAGAC GAAGAC(2/6) BsmBI 468 CGTCTC CGTCTC(1/5) BspHI 401 TCATGA T^CATGA BstAPI 231 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 59 CCACTTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 325 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 440 CCATGG C^C[GA][CT]GG EarI 847 GAAGAG CTCTTC(1/4) Eco47III 9 AGCGCT AGC^GCT EcoNI 563 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 949 GAATTC G^AATTC FspI 686 TGCGCA TGC^GCA Ksp632I 847 GAAGAG CTCTTC(1/4) MaeI 574 CTAG C^TAG MaeIII 253 GTGAC ^GT[ACGT]AC MluNI 56 TGGCCA TGG^CCA MscI 56 TGGCCA TGG^CCA MseI 1015 TTAA T^TAA NcoI 440 CCATGG C^CATGG PflFI 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 261 CACGTG CAC^GTG PmlI 261 CACGTG CAC^GTG PpuMI 163 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 401 TCATGA T^CATGA SbfI 177 CCTGCAGG CCTGCA^GG SexAI 248 ACCAGGT A^CC[AT]GGT Sse8387I 177 CCTGCAGG CCTGCA^GG Tru9I 1015 TTAA T^TAA Tsp45I 253 GTGAC ^GT[GC]AC Tth111I 697 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 706 AAATTC [GA]^AATT[CT] 949 GAATTC AflIII 260 ACACGT A^C[GA][CT]GT 465 ACACGT ApoI 706 AAATTC [GA]^AATT[CT] 949 GAATTC BsaAI 261 CACGTG [CT]AC^GT[GA] 364 TACGTG BseRI 712 GAGGAG GAGGAG(10/8) 754 GAGGAG BsgI 229 CTGCAC GTGCAG(16/14) 381 CTGCAC BsmAI 838 GAGAC GTCTC(1/5) 469 GTCTC BssSI 346 CACGAG CACGAG(-5/-1) 823 CTCGTG BstYI 70 GGATCC [GA]^GATC[CT] 446 AGATCC DdeI 969 CTCAG C^T[ACGT]AG 1029 CTCAG DraII 163 GGGTCCC [GA]G^G[ACGT]CC[CT] 680 AGGCCCT EaeI 56 TGGCCA [CT]^GGCC[GA] 452 CGGCCA EcoO109I 163 GGGTCCC [GA]G^G[ACGT]CC[CT] 680 AGGCCCT EcoT14I 371 CCAAGG C^C[AT][AT]GG 440 CCATGG HaeII 9 AGCGCT [GA]GCGC^[CT] 277 AGCGCC HphI 252 GGTGA GGTGA(8/7) 599 TCACC MflI 70 GGATCC [GA]^GATC[CT] 446 AGATCC PstI 178 CTGCAG CTGCA^G 868 CTGCAG SacI 208 GAGCTC GAGCT^C 349 GAGCTC SfaNI 237 GATGC GCATC(5/9) 503 GCATC SfcI 178 CTGCAG C^T[GA][CT]AG 868 CTGCAG StyI 371 CCAAGG C^C[AT][AT]GG 440 CCATGG XhoII 70 GGATCC [GA]^GATC[CT] 446 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp509I TspRI TthHB8I