(Circular) (Six-base) MAPSORT of: pFN21AE3875.seq Check: 6226 from: 1 to: 5761 pFN21AE3875 5761 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2790 2924 3003 3104 1937 Size: 853 134 79 101 4594 Fragments arranged by size: 4594 853 134 101 79 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2507 2921 3824 5208 5476 1632 Size: 713 162 414 903 1384 268 1917 Fragments arranged by size: 1917 1384 903 713 414 268 162 AfeI AGC'GCT Cuts at: 2016 2016 Size: 5761 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2265 2470 4660 5637 1203 Size: 1062 205 2190 977 1327 Fragments arranged by size: 2190 1327 1062 977 205 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2046 3504 5076 2046 Size: 1458 1572 2731 Fragments arranged by size: 2731 1572 1458 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2711 2954 3070 3296 3448 5557 1493 Size: 1218 243 116 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 243 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 2075 3091 3458 2075 Size: 1016 367 4378 Fragments arranged by size: 4378 1016 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2642 3082 4537 618 Size: 325 217 627 855 440 1455 1842 Fragments arranged by size: 1842 1455 855 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2217 2358 2568 3080 1391 Size: 826 141 210 512 4072 Fragments arranged by size: 4072 826 512 210 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2495 928 Size: 971 596 4194 Fragments arranged by size: 4194 971 596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2465 4372 136 Size: 107 122 71 631 605 793 1907 1525 Fragments arranged by size: 1907 1525 793 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 2376 3822 4978 1790 Size: 586 1446 1156 2573 Fragments arranged by size: 2573 1446 1156 586 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 3492 4450 Size: 416 53 83 399 24 697 1378 958 Cuts at: 4450 442 Size: 1753 Fragments arranged by size: 1753 1378 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 4421 1239 Size: 992 2190 2579 Fragments arranged by size: 2579 2190 992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2268 2371 3552 493 Size: 711 1064 103 1181 2702 Fragments arranged by size: 2702 1181 1064 711 103 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 1178 Size: 30 165 30 1203 30 4303 Fragments arranged by size: 4303 1203 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2732 2774 1186 Size: 1546 42 4173 Fragments arranged by size: 4173 1546 42 BseYI C'CCAG_C Cuts at: 2751 4964 5460 2751 Size: 2213 496 3052 Fragments arranged by size: 3052 2213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2371 1163 Size: 573 483 152 4553 Fragments arranged by size: 4553 573 483 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2358 2982 3822 3907 4978 2217 Size: 141 624 840 85 1071 3000 Fragments arranged by size: 3000 1071 840 624 141 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2479 2479 Size: 5761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2358 2376 2568 2982 3080 3822 Size: 399 427 141 18 192 414 98 742 Cuts at: 3822 3907 4978 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 414 399 192 141 98 85 18 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 2406 3650 5380 2406 Size: 1244 1730 2787 Fragments arranged by size: 2787 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 2351 2828 3821 4833 2351 Size: 477 993 1012 3279 Fragments arranged by size: 3279 1012 993 477 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 4313 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2071 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2075 2451 3091 3458 3843 3860 5312 5398 Size: 103 376 640 367 385 17 1452 86 Cuts at: 5398 5410 5756 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 640 385 376 367 346 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3829 Fragments arranged by size: 3829 1380 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2223 3277 4071 4091 2223 Size: 1054 794 20 3893 Fragments arranged by size: 3893 1054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DraIII CAC_nnn'GTG Cuts at: 2335 2335 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2457 3208 Size: 54 1425 50 275 18 231 396 751 Cuts at: 3208 4097 5726 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 751 396 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2847 3691 1353 Size: 1494 844 3423 Fragments arranged by size: 3423 1494 844 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2467 2575 4348 4722 4868 387 Size: 1064 138 878 108 1773 374 146 1280 Fragments arranged by size: 1773 1280 1064 878 374 146 138 108 EcoICRI GAG'CTC Cuts at: 2215 2356 2215 Size: 141 5620 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2345 2507 2921 3824 4421 5208 Size: 393 599 114 162 414 903 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 903 787 599 597 414 393 268 162 114 EcoNI CCTnn'n_nnAGG Cuts at: 2572 2572 Size: 5761 EcoO109I rG'GnC_Cy Cuts at: 2169 2686 2169 Size: 517 5244 EcoRI G'AATT_C Cuts at: 2954 3070 3448 2954 Size: 116 378 5267 Fragments arranged by size: 5267 378 116 FspI TGC'GCA Cuts at: 2693 4267 2693 Size: 1574 4187 HaeII r_GCGC'y Cuts at: 1678 2018 2286 4908 1678 Size: 340 268 2622 2531 Fragments arranged by size: 2622 2531 340 268 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 4402 4434 4476 Size: 32 135 32 495 32 2303 32 42 Cuts at: 4476 4508 1373 Size: 32 2626 Fragments arranged by size: 2626 2303 495 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 10 Size: 54 1425 343 231 3708 Fragments arranged by size: 3708 1425 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 3719 4078 4237 518 Size: 648 2553 359 159 2042 Fragments arranged by size: 2553 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 2665 2755 2878 2992 3177 3854 5002 Size: 603 456 90 123 114 185 677 1148 Cuts at: 5002 5247 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 603 456 245 185 123 114 90 NcoI C'CATG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3829 Fragments arranged by size: 3829 1380 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2268 2268 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 3830 3862 5343 5375 2957 Size: 32 841 32 1481 32 3343 Fragments arranged by size: 3343 1481 841 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2169 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2187 2877 3113 830 Size: 1357 690 236 3478 Fragments arranged by size: 3478 1357 690 236 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2209 2878 2992 2209 Size: 669 114 4978 Fragments arranged by size: 4978 669 114 SacI G_AGCT'C Cuts at: 2217 2358 2217 Size: 141 5620 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SanDI GG'GwC_CC Cuts at: 2169 2169 Size: 5761 SbfI CC_TGCA'GG Cuts at: 2187 3113 2187 Size: 926 4835 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SexAI A'CCwGG_T Cuts at: 2253 2253 Size: 5761 SfcI C'TryA_G Cuts at: 826 1045 2183 2873 3109 4244 4925 5116 826 Size: 219 1138 690 236 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 690 681 236 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2376 2445 3200 513 Size: 552 1311 69 755 3074 Fragments arranged by size: 3074 1311 755 552 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2133 2621 2693 2765 3788 3805 3958 Size: 1255 553 488 72 72 1023 17 153 Cuts at: 3958 4143 4588 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1023 553 488 445 185 153 72 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 2502 2613 Size: 274 80 33 51 1443 456 69 111 Cuts at: 2613 4007 5719 96 Size: 1394 1712 138 Fragments arranged by size: 1712 1443 1394 456 274 138 111 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 2705 1316 Size: 1389 4372 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI