################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 322 GTAGAC GT^[AC][GT]AC AccII 15 CGCG CG^CG AfaI 646 GTAC GT^AC AflII 882 CTTAAG C^TTAAG AlwI 1003 GATCC GGATC(4/5) Asp700I 124 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 827 GTGCTC G[AT]GC[AT]^C BclI 531 TGATCA T^GATCA BfaI 643 CTAG C^TAG BfrI 882 CTTAAG C^TTAAG BglII 603 AGATCT A^GATCT BpmI 609 CTCCAG CTGGAG(16/14) BseRI 1109 GAGGAG GAGGAG(10/8) BsiHKAI 827 GTGCTC G[AT]GC[AT]^C BsiYI 457 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 457 CCTGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 423 GGGAC GGGAC(10/14) BsmI 1130 GCATTC GAATGC(1/-1) BstAPI 820 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 15 CGCG CG^CG BstXI 569 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 603 AGATCT [GA]^GATC[CT] DraI 304 TTTAAA TTT^AAA EarI 1074 CTCTTC CTCTTC(1/4) FauI 16 GCGGG CCCGC(4/6) FbaI 531 TGATCA T^GATCA HindIII 44 AAGCTT A^AGCTT Ksp632I 1074 CTCTTC CTCTTC(1/4) MaeI 643 CTAG C^TAG MflI 603 AGATCT [GA]^GATC[CT] MslI 523 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 514 CAGCTG C[AC]G^C[GT]G MvnI 15 CGCG CG^CG PaeR7I 839 CTCGAG C^TCGAG PvuII 514 CAGCTG CAG^CTG RsaI 646 GTAC GT^AC SfcI 949 CTACAG C^T[GA][CT]AG SmaI 420 CCCGGG CCC^GGG TaqI 840 TCGA T^CGA TthHB8I 840 TCGA T^CGA XhoI 839 CTCGAG C^TCGAG XhoII 603 AGATCT [GA]^GATC[CT] XmaCI 420 CCCGGG C^CCGGG XmaI 420 CCCGGG C^CCGGG XmnI 124 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 7 GCGG CCGC(-3/-1) 16 GCGG AlwNI 140 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 344 CAGAAGCTG AspI 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1152 GACCCAGTC AvaI 420 CCCGGG C^[CT]CG[GA]G 839 CTCGAG BanII 499 GGGCTC G[GA]GC[CT]^C 842 GAGCCC BsoBI 420 CCCGGG C^[CT]CG[GA]G 839 CTCGAG HapII 421 CCGG C^CGG 593 CCGG HpaII 421 CCGG C^CGG 593 CCGG MspI 421 CCGG C^CGG 593 CCGG MwoI 7 GCGGAGAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 820 GCAAGATGTGC NdeI 523 CATATG CA^TATG 1022 CATATG PflFI 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1152 GACCCAGTC PleI 366 GACTC GAGTC(4/5) 241 GAGTC SfaNI 967 GATGC GCATC(5/9) 447 GCATC TfiI 774 GATTC G^A[AT]TC 1114 GAATC Tth111I 397 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1152 GACCCAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AluI ApoI AvaII BbvI BcnI BmyI BsaJI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MaeII MboI MboII MnlI MseI MvaI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SmlI Tru9I TseI Tsp509I TspRI