################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AciI AclI AcyI AfaI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstF5I BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccI 282 GTATAC GT^[AC][GT]AC AccIII 10 TCCGGA T^CCGGA AcsI 607 GAATTT [GA]^AATT[CT] AflIII 324 ACATGT A^C[GA][CT]GT Alw44I 596 GTGCAC G^TGCAC AlwI 109 GGATC GGATC(4/5) AlwNI 382 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 596 GTGCAC G^TGCAC ApoI 607 GAATTT [GA]^AATT[CT] AspHI 596 GTGCAC G[AT]GC[AT]^C BbvCI 427 GCTGAGG CCTCAGC(-5/-2) BbvI 660 GCAGC GCAGC(8/12) BglII 99 AGATCT A^GATCT BmyI 596 GTGCAC G[AGT]GC[ACT]^C BsaJI 287 CCAAGG C^C[ACGT][ACGT]GG BsaWI 10 TCCGGA [AT]^CCGG[AT] BseAI 10 TCCGGA T^CCGGA BsgI 208 GTGCAG GTGCAG(16/14) BsiHKAI 596 GTGCAC G[AT]GC[AT]^C Bsp1286I 596 GTGCAC G[AGT]GC[ACT]^C BspEI 10 TCCGGA T^CCGGA BspHI 576 TCATGA T^CATGA BspLU11I 324 ACATGT A^CATGT BsrI 592 ACTGG ACTGG(1/-1) BssKI 26 CCAGG ^CC[ACGT]GG Bst1107I 282 GTATAC GTA^TAC BstAPI 210 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 26 CCAGG CC^[AT]GG BstYI 99 AGATCT [GA]^GATC[CT] BstZ17I 282 GTATAC GTA^TAC DraI 610 TTTAAA TTT^AAA EcoRII 26 CCAGG ^CC[AT]GG EcoT14I 287 CCAAGG C^C[AT][AT]GG Fnu4HI 660 GCAGC GC^[ACGT]GC HapII 11 CCGG C^CGG HincII 235 GTTGAC GT[CT]^[GA]AC HindII 235 GTTGAC GT[CT]^[GA]AC HindIII 358 AAGCTT A^AGCTT HpaII 11 CCGG C^CGG HphI 202 GGTGA GGTGA(8/7) ItaI 660 GCAGC GC^[ACGT]GC MaeII 156 ACGT A^CGT MaeIII 308 GTAAC ^GT[ACGT]AC MflI 99 AGATCT [GA]^GATC[CT] MroI 10 TCCGGA T^CCGGA MspI 11 CCGG C^CGG MvaI 26 CCAGG CC^[AT]GG NspI 324 ACATGT [GA]CATG^[CT] PshAI 138 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 576 TCATGA T^CATGA SapI 250 GCTCTTC GCTCTTC(1/4) ScrFI 26 CCAGG CC^[ACGT]GG SpeI 304 ACTAGT A^CTAGT SspI 371 AATATT AAT^ATT StyI 287 CCAAGG C^C[AT][AT]GG TaqI 462 TCGA T^CGA TseI 660 GCAGC G^C[AT]GC TspRI 403 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 462 TCGA T^CGA XbaI 490 TCTAGA T^CTAGA XhoII 99 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 359 AGCT AG^CT 426 AGCT BbsI 135 GAAGAC GAAGAC(2/6) 171 GTCTTC BpuAI 135 GAAGAC GAAGAC(2/6) 171 GTCTTC HinfI 477 GATTC G^A[ACGT]TC 573 GAATC MwoI 210 GCAGAATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 250 GCTCTTCTTGC PstI 140 CTGCAG CTGCA^G 176 CTGCAG SfcI 140 CTGCAG C^T[GA][CT]AG 176 CTGCAG TfiI 477 GATTC G^A[AT]TC 573 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BfaI BsmAI DdeI DpnII EarI Ksp632I MaeI MboI MboII MnlI MseI NdeII NlaIII Sau3AI Tru9I Tsp509I