(Circular) (Six-base) MAPSORT of: pFN21AE1727.seq Check: 3797 from: 1 to: 5413 pFN21AE1727 5413 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3223 278 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2288 2756 1937 Size: 351 468 4594 Fragments arranged by size: 4594 468 351 Acc65I G'GTAC_C Cuts at: 2734 2734 Size: 5413 AclI AA'CG_TT Cuts at: 1563 3540 3913 1563 Size: 1977 373 3063 Fragments arranged by size: 3063 1977 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 3476 4860 5128 1632 Size: 480 1364 1384 268 1917 Fragments arranged by size: 1917 1384 1364 480 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5216 AflIII A'CryG_T Cuts at: 1203 2329 4312 5289 1203 Size: 1126 1983 977 1327 Fragments arranged by size: 1983 1327 1126 977 AgeI A'CCGG_T Cuts at: 4244 4244 Size: 5413 AhdI GACnn_n'nnGTC Cuts at: 4142 4142 Size: 5413 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5413 AlwNI CAG_nnn'CTG Cuts at: 2392 3156 4728 2392 Size: 764 1572 3077 Fragments arranged by size: 3077 1572 764 ApaLI G'TGCA_C Cuts at: 2601 3470 4626 2601 Size: 869 1156 3388 Fragments arranged by size: 3388 1156 869 ApoI r'AATT_y Cuts at: 1493 2612 2722 2948 3100 5209 1493 Size: 1119 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 1119 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 3967 160 Size: 872 2935 1606 Fragments arranged by size: 2935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 AvaI C'yCGr_G Cuts at: 1870 1943 2738 1870 Size: 73 795 4545 Fragments arranged by size: 4545 795 73 BamHI G'GATC_C Cuts at: 2743 3110 2743 Size: 367 5046 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 4189 618 Size: 325 217 627 947 1455 1842 Fragments arranged by size: 1842 1455 947 627 325 217 BanII G_rGCy'C Cuts at: 1391 2732 1391 Size: 1341 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2147 2169 928 Size: 971 248 22 4172 Fragments arranged by size: 4172 971 248 22 BbvCI CC'TCA_GC Cuts at: 2433 2433 Size: 5413 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 1903 Size: 34 1667 34 3678 Fragments arranged by size: 3678 1667 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4515 3305 Size: 1210 4203 BclI T'GATC_A Cuts at: 1548 1548 Size: 5413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4024 136 Size: 107 122 71 631 605 2352 1525 Fragments arranged by size: 2352 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2104 5408 2104 Size: 3304 2109 BlpI GC'TnA_GC Cuts at: 2830 2830 Size: 5413 Bme1580I G_kGCm'C Cuts at: 1790 2605 3474 4630 1790 Size: 815 869 1156 2573 Fragments arranged by size: 2573 1156 869 815 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5413 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3144 4102 442 Size: 416 53 83 399 24 1727 958 1753 Fragments arranged by size: 1753 1727 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4073 1239 Size: 2834 2579 Bpu10I CC'TnA_GC Cuts at: 2433 2433 Size: 5413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3538 4403 4701 3538 Size: 865 298 4250 Fragments arranged by size: 4250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 882 Size: 617 2577 2219 Fragments arranged by size: 2577 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3204 493 Size: 711 2000 2702 Fragments arranged by size: 2702 2000 711 BsaBI GATnn'nnATC Cuts at: 5407 5407 Size: 5413 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3220 3602 4237 275 Size: 53 83 186 936 1687 382 635 1451 Fragments arranged by size: 1687 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2015 3846 4244 4518 4665 5076 2015 Size: 1831 398 274 147 411 2352 Fragments arranged by size: 2352 1831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 1178 Size: 30 165 30 820 30 4338 Fragments arranged by size: 4338 820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5413 BseYI C'CCAG_C Cuts at: 4616 5112 4616 Size: 496 4917 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 1163 Size: 573 498 4342 Fragments arranged by size: 4342 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2863 3624 4652 1540 Size: 275 186 862 761 1028 2301 Fragments arranged by size: 2301 1028 862 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2605 3474 3559 4630 2605 Size: 869 85 1071 3388 Fragments arranged by size: 3388 1071 869 85 BsmI GAATG_Cn' Cuts at: 2924 3017 2924 Size: 93 5320 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2605 2732 3474 3559 4630 1391 Size: 399 815 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 815 742 399 127 85 BspEI T'CCGG_A Cuts at: 2015 5076 2015 Size: 3061 2352 BspHI T'CATG_A Cuts at: 2581 3302 5032 2581 Size: 721 1730 2962 Fragments arranged by size: 2962 1730 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2768 844 Size: 1924 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3300 1394 Size: 429 1477 3507 Fragments arranged by size: 3507 1477 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3908 4082 65 Size: 1183 2660 174 1396 Fragments arranged by size: 2660 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4057 4244 1700 Size: 110 2247 187 2869 Fragments arranged by size: 2869 2247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5413 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5413 BssSI C'ACGA_G Cuts at: 3473 4485 3473 Size: 1012 4401 BstAPI GCAn_nnn'nTGC Cuts at: 794 2221 794 Size: 1427 3986 BstBI TT'CG_AA Cuts at: 4217 4217 Size: 5413 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5413 BstYI r'GATC_y Cuts at: 1972 2104 2743 3110 3495 3512 4964 5050 5062 Size: 132 639 367 385 17 1452 86 12 Cuts at: 5062 5408 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 639 385 367 346 132 86 17 12 BstZ17I GTA'TAC Cuts at: 2289 2289 Size: 5413 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4674 Fragments arranged by size: 4674 683 56 BtsI GCAGTG_nn' Cuts at: 2414 2929 3723 3743 2414 Size: 515 794 20 4084 Fragments arranged by size: 4084 794 515 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5413 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5378 DraI TTT'AAA Cuts at: 2617 2717 2617 Size: 100 5313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4420 809 Size: 507 3104 1802 Fragments arranged by size: 3104 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378 Size: 54 1425 50 275 18 1030 889 1629 Cuts at: 5378 8 Size: 43 Fragments arranged by size: 1629 1425 1030 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2860 1537 Size: 275 1048 4090 Fragments arranged by size: 4090 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2559 2700 3343 1353 Size: 909 297 141 643 3423 Fragments arranged by size: 3423 909 643 297 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4000 4374 4520 387 Size: 1064 138 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 3476 4073 4860 5128 1239 Size: 393 480 1364 597 787 268 1524 Fragments arranged by size: 1524 1364 787 597 480 393 268 EcoRI G'AATT_C Cuts at: 2722 3100 2722 Size: 378 5035 FspI TGC'GCA Cuts at: 3919 3919 Size: 5413 HaeII r_GCGC'y Cuts at: 1678 4560 1678 Size: 2882 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2644 2676 4054 4086 4128 Size: 32 135 32 1072 32 1378 32 42 Cuts at: 4128 4160 1373 Size: 32 2626 Fragments arranged by size: 2626 1378 1072 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 2757 3003 669 Size: 988 281 304 515 246 3079 Fragments arranged by size: 3079 988 515 304 281 246 HindIII A'AGCT_T Cuts at: 748 2363 748 Size: 1615 3798 HpaI GTT'AAC Cuts at: 3003 3003 Size: 5413 KpnI G_GTAC'C Cuts at: 2738 2738 Size: 5413 MfeI C'AATT_G Cuts at: 3012 3012 Size: 5413 MluI A'CGCG_T Cuts at: 5289 5289 Size: 5413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2215 2550 4527 4711 1305 Size: 910 335 1977 184 2007 Fragments arranged by size: 2007 1977 910 335 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3591 Fragments arranged by size: 3591 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3371 3730 3889 518 Size: 648 2205 359 159 2042 Fragments arranged by size: 2205 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2829 3506 4654 4899 1606 Size: 1223 677 1148 245 2120 Fragments arranged by size: 2120 1223 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3922 Fragments arranged by size: 3922 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5413 NotI GC'GGCC_GC Cuts at: 2860 2860 Size: 5413 NspI r_CATG'y Cuts at: 1132 1229 2333 2771 4316 1132 Size: 97 1104 438 1545 2229 Fragments arranged by size: 2229 1545 1104 438 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5413 PciI A'CATG_T Cuts at: 2329 4312 2329 Size: 1983 3430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5413 PfoI T'CCnGG_A Cuts at: 2030 2030 Size: 5413 PmeI GTTT'AAAC Cuts at: 2717 2717 Size: 5413 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4995 5027 3482 Size: 32 1481 32 3868 Fragments arranged by size: 3868 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2147 1613 Size: 534 4879 PsiI TTA'TAA Cuts at: 2983 2983 Size: 5413 PstI C_TGCA'G Cuts at: 830 2149 2185 2765 830 Size: 1319 36 580 3478 Fragments arranged by size: 3478 1319 580 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5413 SalI G'TCGA_C Cuts at: 1936 2755 1936 Size: 819 4594 SapI GCTCTTCn'nnn_ Cuts at: 2262 2262 Size: 5413 SbfI CC_TGCA'GG Cuts at: 2765 2765 Size: 5413 ScaI AGT'ACT Cuts at: 3661 5373 3661 Size: 1712 3701 SfcI C'TryA_G Cuts at: 826 1045 2145 2181 2761 3896 4577 4768 826 Size: 219 1100 36 580 1135 681 191 1471 Fragments arranged by size: 1471 1135 1100 681 580 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5413 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5413 SmaI CCC'GGG Cuts at: 1872 2740 1872 Size: 868 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3517 4418 4680 820 Size: 197 926 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3204 493 Size: 2711 2702 SpeI A'CTAG_T Cuts at: 152 2309 152 Size: 2157 3256 SphI G_CATG'C Cuts at: 1132 2771 1132 Size: 1639 3774 SspI AAT'ATT Cuts at: 5 52 2378 3337 5 Size: 47 2326 959 2081 Fragments arranged by size: 2326 2081 959 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5413 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 2852 513 Size: 552 939 288 560 3074 Fragments arranged by size: 3074 939 560 552 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3440 3457 3610 3795 4240 325 Size: 1255 1860 17 153 185 445 1498 Fragments arranged by size: 1860 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3659 5371 96 Size: 274 80 33 51 1443 1682 1712 138 Fragments arranged by size: 1712 1682 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5373 5405 5373 Size: 32 5381 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5413 XbaI T'CTAG_A Cuts at: 2495 2749 2495 Size: 254 5159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5413 XmaI C'CCGG_G Cuts at: 1870 2738 1870 Size: 868 4545 XmnI GAAnn'nnTTC Cuts at: 3542 3542 Size: 5413 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3221 276 Size: 53 83 186 936 1687 2468 Fragments arranged by size: 2468 1687 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI