(Linear) (Six-base) MAPSORT of: KIEE1727.seq  Check: 3718  from: 1  to: 708



KIEE1727.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

AccI GT'mk_AC

Cuts at:      0     283     708 
   Size:        283     425
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     708 
   Size:        107     601
 
AflIII A'CryG_T

Cuts at:      0     324     708 
   Size:        324     384
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387     708 
   Size:        387     321
 
ApaLI G'TGCA_C

Cuts at:      0     596     708 
   Size:        596     112
 
ApoI r'AATT_y

Cuts at:      0     607     708 
   Size:        607     101
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     142     164     708 
   Size:        142      22     544

  Fragments arranged by size: 

                 544     142      22
 
BbvCI CC'TCA_GC

Cuts at:      0     428     708 
   Size:        428     280
 
BglII A'GATC_T

Cuts at:      0      99     708 
   Size:         99     609
 
Bme1580I G_kGCm'C

Cuts at:      0     600     708 
   Size:        600     108
 
Bpu10I CC'TnA_GC

Cuts at:      0     428     708 
   Size:        428     280
 
BsaWI w'CCGG_w

Cuts at:      0      10     708 
   Size:         10     698
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     218     248     708 
   Size:        218      30     460

  Fragments arranged by size: 

                 460     218      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     708 
   Size:        229     479
 
BsiHKAI G_wGCw'C

Cuts at:      0     600     708 
   Size:        600     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     600     708 
   Size:        600     108
 
BspEI T'CCGG_A

Cuts at:      0      10     708 
   Size:         10     698
 
BspHI T'CATG_A

Cuts at:      0     576     708 
   Size:        576     132
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     216     708 
   Size:        216     492
 
BstYI r'GATC_y

Cuts at:      0      99     708 
   Size:         99     609
 
BstZ17I GTA'TAC

Cuts at:      0     284     708 
   Size:        284     424
 
BtsI GCAGTG_nn'

Cuts at:      0     409     708 
   Size:        409     299
 
DraI TTT'AAA

Cuts at:      0     612     708 
   Size:        612      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     554     695     708 
   Size:        257     297     141      13

  Fragments arranged by size: 

                 297     257     141      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     708 
   Size:        107     601
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     639     671     708 
   Size:        639      32      37

  Fragments arranged by size: 

                 639      37      32
 
HincII GTy'rAC

Cuts at:      0     237     708 
   Size:        237     471
 
HindIII A'AGCT_T

Cuts at:      0     358     708 
   Size:        358     350
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     545     708 
   Size:        210     335     163

  Fragments arranged by size: 

                 335     210     163
 
NspI r_CATG'y

Cuts at:      0     328     708 
   Size:        328     380
 
PciI A'CATG_T

Cuts at:      0     324     708 
   Size:        324     384
 
PfoI T'CCnGG_A

Cuts at:      0      25     708 
   Size:         25     683
 
PshAI GACnn'nnGTC

Cuts at:      0     142     708 
   Size:        142     566
 
PstI C_TGCA'G

Cuts at:      0     144     180     708 
   Size:        144      36     528

  Fragments arranged by size: 

                 528     144      36
 
SapI GCTCTTCn'nnn_

Cuts at:      0     257     708 
   Size:        257     451
 
SfcI C'TryA_G

Cuts at:      0     140     176     708 
   Size:        140      36     532

  Fragments arranged by size: 

                 532     140      36
 
SpeI A'CTAG_T

Cuts at:      0     304     708 
   Size:        304     404
 
SspI AAT'ATT

Cuts at:      0     373     708 
   Size:        373     335
 
StyI C'CwwG_G

Cuts at:      0     287     708 
   Size:        287     421
 
XbaI T'CTAG_A

Cuts at:      0     490     708 
   Size:        490     218
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI    ApaLI     ApoI     BbsI    BbvCI
    BglII Bme1580I   Bpu10I    BsaWI    BsaXI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspHI   BstAPI    BstYI  BstZ17I     BtsI     DraI     EarI
  Eco57MI    Hin4I   HincII  HindIII     MmeI     NspI     PciI     PfoI
    PshAI     PstI     SapI     SfcI     SpeI     SspI     StyI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI