(Circular) (Six-base) MAPSORT of: pFN21AE6493.seq Check: 7088 from: 1 to: 6535 pFN21AE6493 6535 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4345 278 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2238 2429 3878 1937 Size: 301 191 1449 4594 Fragments arranged by size: 4594 1449 301 191 Acc65I G'GTAC_C Cuts at: 2916 3856 2916 Size: 940 5595 AclI AA'CG_TT Cuts at: 1563 4662 5035 1563 Size: 3099 373 3063 Fragments arranged by size: 3099 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3106 3559 4598 5982 6250 1632 Size: 1474 453 1039 1384 268 1917 Fragments arranged by size: 1917 1474 1384 1039 453 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6338 AflIII A'CryG_T Cuts at: 1203 5434 6411 1203 Size: 4231 977 1327 Fragments arranged by size: 4231 1327 977 AgeI A'CCGG_T Cuts at: 5366 5366 Size: 6535 AhdI GACnn_n'nnGTC Cuts at: 5264 5264 Size: 6535 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6535 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3754 3786 3754 Size: 32 6503 AlwNI CAG_nnn'CTG Cuts at: 3095 4278 5850 3095 Size: 1183 1572 3780 Fragments arranged by size: 3780 1572 1183 ApaLI G'TGCA_C Cuts at: 4592 5748 4592 Size: 1156 5379 ApoI r'AATT_y Cuts at: 1493 2686 2695 3466 3844 4070 4222 6331 1493 Size: 1193 9 771 378 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 771 378 226 152 9 AseI AT'TA_AT Cuts at: 160 1032 3075 5089 160 Size: 872 2043 2014 1606 Fragments arranged by size: 2043 2014 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 AvaI C'yCGr_G Cuts at: 1870 1943 3860 1870 Size: 73 1917 4545 Fragments arranged by size: 4545 1917 73 BamHI G'GATC_C Cuts at: 3865 4232 3865 Size: 367 6168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2222 2916 3856 5311 Size: 325 217 627 240 195 694 940 1455 Cuts at: 5311 618 Size: 1842 Fragments arranged by size: 1842 1455 940 694 627 325 240 217 195 BanII G_rGCy'C Cuts at: 1391 2901 3187 3385 3854 1391 Size: 1510 286 198 469 4072 Fragments arranged by size: 4072 1510 469 286 198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2302 928 Size: 971 403 5161 Fragments arranged by size: 5161 971 403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4726 4760 1903 Size: 34 2789 34 3678 Fragments arranged by size: 3678 2789 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3606 4427 5637 3606 Size: 821 1210 4504 Fragments arranged by size: 4504 1210 821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6535 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5146 136 Size: 107 122 71 631 605 3474 1525 Fragments arranged by size: 3474 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3103 6530 3103 Size: 3427 3108 BlpI GC'TnA_GC Cuts at: 3952 3952 Size: 6535 Bme1580I G_kGCm'C Cuts at: 1790 2172 2225 4596 5752 1790 Size: 382 53 2371 1156 2573 Fragments arranged by size: 2573 2371 1156 382 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2342 2549 4266 Size: 416 53 83 399 24 925 207 1717 Cuts at: 4266 5224 442 Size: 958 1753 Fragments arranged by size: 1753 1717 958 925 416 399 207 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2196 1056 Size: 1140 5395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3052 5195 1239 Size: 1813 2143 2579 Fragments arranged by size: 2579 2143 1813 Bpu10I CC'TnA_GC Cuts at: 3082 3082 Size: 6535 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4660 5525 5823 4660 Size: 865 298 5372 Fragments arranged by size: 5372 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3147 5198 882 Size: 617 1648 2051 2219 Fragments arranged by size: 2219 2051 1648 617 BsaAI yAC'GTr Cuts at: 493 1204 2050 4326 493 Size: 711 846 2276 2702 Fragments arranged by size: 2702 2276 846 711 BsaBI GATnn'nnATC Cuts at: 2605 6529 2605 Size: 3924 2611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3212 4342 4724 5359 Size: 53 83 186 936 1679 1130 382 635 Cuts at: 5359 275 Size: 1451 Fragments arranged by size: 1679 1451 1130 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4968 5366 5640 5787 6198 4968 Size: 398 274 147 411 5305 Fragments arranged by size: 5305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3318 3348 1178 Size: 30 165 30 1915 30 4365 Fragments arranged by size: 4365 1915 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6535 BseYI C'CCAG_C Cuts at: 2108 5738 6234 2108 Size: 3630 496 2409 Fragments arranged by size: 3630 2409 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2506 2518 2816 2940 1163 Size: 573 770 12 298 124 4758 Fragments arranged by size: 4758 770 573 298 124 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 2152 3985 4746 5774 1540 Size: 275 186 81 70 1833 761 1028 2301 Fragments arranged by size: 2301 1833 1028 761 275 186 81 70 BsiHKAI G_wGCw'C Cuts at: 3331 3385 4596 4681 5752 3331 Size: 54 1211 85 1071 4114 Fragments arranged by size: 4114 1211 1071 85 54 BsmI GAATG_Cn' Cuts at: 4046 4139 4046 Size: 93 6442 BsmBI CGTCTCn'nnnn_ Cuts at: 2393 2393 Size: 6535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2225 2901 3187 3331 3385 3854 Size: 399 382 53 676 286 144 54 469 Cuts at: 3854 4596 4681 5752 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 676 469 399 382 286 144 85 54 53 BspEI T'CCGG_A Cuts at: 6198 6198 Size: 6535 BspHI T'CATG_A Cuts at: 4424 6154 4424 Size: 1730 4805 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3890 844 Size: 3046 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4422 1394 Size: 429 2599 3507 Fragments arranged by size: 3507 2599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3413 3419 3826 5030 5204 65 Size: 1183 2165 6 407 1204 174 1396 Fragments arranged by size: 2165 1396 1204 1183 407 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 5179 5366 1700 Size: 110 3369 187 2869 Fragments arranged by size: 3369 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6535 BssSI C'ACGA_G Cuts at: 4595 5607 4595 Size: 1012 5523 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6535 BstBI TT'CG_AA Cuts at: 5339 5339 Size: 6535 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6535 BstYI r'GATC_y Cuts at: 1972 3103 3865 4232 4617 4634 6086 6172 6184 Size: 1131 762 367 385 17 1452 86 12 Cuts at: 6184 6530 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1131 762 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2430 2430 Size: 6535 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 2172 513 Size: 552 939 11 157 4876 Fragments arranged by size: 4876 939 552 157 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5796 Fragments arranged by size: 5796 683 56 BtsI GCAGTG_nn' Cuts at: 2172 4051 4845 4865 2172 Size: 1879 794 20 3842 Fragments arranged by size: 3842 1879 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2496 2531 529 Size: 35 1932 35 4533 Fragments arranged by size: 4533 1932 35 35 DraI TTT'AAA Cuts at: 2658 3839 2658 Size: 1181 5354 DraIII CAC_nnn'GTG Cuts at: 2543 2543 Size: 6535 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5542 809 Size: 507 4226 1802 Fragments arranged by size: 4226 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2116 2149 Size: 54 1425 50 275 18 249 37 33 Cuts at: 2149 3982 4871 6500 8 Size: 1833 889 1629 43 Fragments arranged by size: 1833 1629 1425 889 275 249 54 50 43 37 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2079 2149 3982 1537 Size: 275 267 70 1833 4090 Fragments arranged by size: 4090 1833 275 267 70 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 4465 1353 Size: 1058 2054 3423 Fragments arranged by size: 3423 2054 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5122 5496 5642 387 Size: 1064 138 3533 374 146 1280 Fragments arranged by size: 3533 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3383 3383 Size: 6535 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3052 3106 3559 4598 5195 5982 6250 Size: 393 1420 54 453 1039 597 787 268 Cuts at: 6250 1239 Size: 1524 Fragments arranged by size: 1524 1420 1039 787 597 453 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2751 2751 Size: 6535 EcoRI G'AATT_C Cuts at: 3844 4222 3844 Size: 378 6157 EcoRV GAT'ATC Cuts at: 3433 3433 Size: 6535 FspI TGC'GCA Cuts at: 5041 5041 Size: 6535 HaeII r_GCGC'y Cuts at: 1678 2180 5682 1678 Size: 502 3502 2531 Fragments arranged by size: 3502 2531 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3674 3706 5176 5208 5250 Size: 32 135 32 2102 32 1470 32 42 Cuts at: 5250 5282 1373 Size: 32 2626 Fragments arranged by size: 2626 2102 1470 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2782 3879 4125 669 Size: 988 281 844 1097 246 3079 Fragments arranged by size: 3079 1097 988 844 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6535 HpaI GTT'AAC Cuts at: 4125 4125 Size: 6535 KpnI G_GTAC'C Cuts at: 2920 3860 2920 Size: 940 5595 MfeI C'AATT_G Cuts at: 3321 4134 3321 Size: 813 5722 MluI A'CGCG_T Cuts at: 6411 6411 Size: 6535 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3352 3565 5649 5833 1305 Size: 2047 213 2084 184 2007 Fragments arranged by size: 2084 2047 2007 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4713 Fragments arranged by size: 4713 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2925 3485 4493 4852 5011 518 Size: 648 867 892 560 1008 359 159 2042 Fragments arranged by size: 2042 1008 892 867 648 560 359 159 MspA1I CmG'CkG Cuts at: 1606 3951 4628 5776 6021 1606 Size: 2345 677 1148 245 2120 Fragments arranged by size: 2345 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5044 Fragments arranged by size: 5044 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6535 NheI G'CTAG_C Cuts at: 1052 2192 1052 Size: 1140 5395 NotI GC'GGCC_GC Cuts at: 2149 3982 2149 Size: 1833 4702 NspI r_CATG'y Cuts at: 1132 1229 2068 2883 3725 3893 5438 1132 Size: 97 839 815 842 168 1545 2229 Fragments arranged by size: 2229 1545 842 839 815 168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6535 PciI A'CATG_T Cuts at: 5434 5434 Size: 6535 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6535 PfoI T'CCnGG_A Cuts at: 3007 3016 3007 Size: 9 6526 PmeI GTTT'AAAC Cuts at: 3839 3839 Size: 6535 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4604 4636 6117 6149 4604 Size: 32 1481 32 4990 Fragments arranged by size: 4990 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2751 2751 Size: 6535 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6535 PsiI TTA'TAA Cuts at: 4105 4105 Size: 6535 PstI C_TGCA'G Cuts at: 830 3887 830 Size: 3057 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6535 SacI G_AGCT'C Cuts at: 3385 3385 Size: 6535 SalI G'TCGA_C Cuts at: 1936 3877 1936 Size: 1941 4594 SbfI CC_TGCA'GG Cuts at: 3887 3887 Size: 6535 ScaI AGT'ACT Cuts at: 4783 6495 4783 Size: 1712 4823 SfcI C'TryA_G Cuts at: 826 1045 3883 5018 5699 5890 826 Size: 219 2838 1135 681 191 1471 Fragments arranged by size: 2838 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6535 SmaI CCC'GGG Cuts at: 1872 3862 1872 Size: 1990 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4639 5540 5802 820 Size: 197 926 2696 901 262 1553 Fragments arranged by size: 2696 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4326 493 Size: 3833 2702 SpeI A'CTAG_T Cuts at: 152 3500 152 Size: 3348 3187 SphI G_CATG'C Cuts at: 1132 2068 3893 1132 Size: 936 1825 3774 Fragments arranged by size: 3774 1825 936 SspI AAT'ATT Cuts at: 5 52 4459 5 Size: 47 4407 2081 Fragments arranged by size: 4407 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6535 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 2933 3160 3436 3974 513 Size: 552 939 126 803 227 276 538 3074 Fragments arranged by size: 3074 939 803 552 538 276 227 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2531 4562 4579 4732 4917 5362 325 Size: 1255 951 2031 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 951 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3570 4781 6493 Size: 274 80 33 51 1443 1593 1211 1712 Cuts at: 6493 96 Size: 138 Fragments arranged by size: 1712 1593 1443 1211 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3012 3044 3156 3188 6495 6527 3012 Size: 32 112 32 3307 32 3020 Fragments arranged by size: 3307 3020 112 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6535 XbaI T'CTAG_A Cuts at: 3871 3871 Size: 6535 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6535 XmaI C'CCGG_G Cuts at: 1870 3860 1870 Size: 1990 4545 XmnI GAAnn'nnTTC Cuts at: 4664 4664 Size: 6535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4343 276 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI