################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MamI MluI MroI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 365 GACGTC GACGT^C AcyI 365 GACGTC G[GA]^CG[CT]C AflII 900 CTTAAG C^TTAAG AflIII 1116 ACATGT A^C[GA][CT]GT Alw44I 1168 GTGCAC G^TGCAC AlwI 451 GGATC GGATC(4/5) ApaLI 1168 GTGCAC G^TGCAC AseI 469 ATTAAT AT^TAAT AsnI 469 ATTAAT AT^TAAT AspI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 1020 GAGCTC G[GA]GC[CT]^C BciVI 1486 GGATAC GTATCC(6/5) BclI 105 TGATCA T^GATCA BcnI 196 CCGGG CC^[GC]GG BfrI 900 CTTAAG C^TTAAG BglII 858 AGATCT A^GATCT BsaHI 365 GACGTC G[GA]^CG[CT]C BsaI 1714 GGTCTC GGTCTC(1/5) BsgI 269 CTGCAC GTGCAG(16/14) BsmI 1680 GAATGC GAATGC(1/-1) Bsp1407I 1047 TGTACA T^GTACA BspHI 722 TCATGA T^CATGA BspLU11I 1116 ACATGT A^CATGT BsrDI 905 GCAATG GCAATG(2/0) BsrGI 1047 TGTACA T^GTACA BssSI 578 CACGAG CACGAG(-5/-1) BstAPI 1007 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 858 AGATCT [GA]^GATC[CT] DraI 409 TTTAAA TTT^AAA DraII 632 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 1519 CTCTTC CTCTTC(1/4) EcoO109I 632 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1591 GAATTC G^AATTC FauI 1030 GCGGG CCCGC(4/6) FbaI 105 TGATCA T^GATCA HapII 196 CCGG C^CGG Hin1I 365 GACGTC G[GA]^CG[CT]C HpaII 196 CCGG C^CGG Ksp632I 1519 CTCTTC CTCTTC(1/4) MaeII 366 ACGT A^CGT MfeI 1365 CAATTG C^AATTG MflI 858 AGATCT [GA]^GATC[CT] MspI 196 CCGG C^CGG MunI 1365 CAATTG C^AATTG NciI 196 CCGGG CC^[GC]GG NspI 1116 ACATGT [GA]CATG^[CT] PflFI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 632 AGGACCT [GA]G^G[AT]CC[CT] PshBI 469 ATTAAT AT^TAAT PvuII 758 CAGCTG CAG^CTG RcaI 722 TCATGA T^CATGA SacI 1020 GAGCTC GAGCT^C ScaI 1515 AGTACT AGT^ACT SmlI 900 CTTAAG C^T[CT][GA]AG SspBI 1047 TGTACA T^GTACA SspI 727 AATATT AAT^ATT Tth111I 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 858 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 313 GGACC G^G[AT]CC 633 GGACC BalI 176 TGGCCA TGG^CCA 815 TGGCCA BbsI 1370 GTCTTC GAAGAC(2/6) 1382 GTCTTC BfaI 1249 CTAG C^TAG 1543 CTAG BpuAI 1370 GTCTTC GAAGAC(2/6) 1382 GTCTTC BsaJI 117 CCAAGG C^C[ACGT][ACGT]GG 309 CCAAGG BsiYI 304 CCAGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1316 CCCAGTGCTGG BslI 304 CCAGACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1316 CCCAGTGCTGG BsmAI 350 GAGAC GTCTC(1/5) 1715 GTCTC EaeI 176 TGGCCA [CT]^GGCC[GA] 815 TGGCCA EcoT14I 117 CCAAGG C^C[AT][AT]GG 309 CCAAGG HindIII 897 AAGCTT A^AGCTT 1280 AAGCTT MaeI 1249 CTAG C^TAG 1543 CTAG MluNI 176 TGGCCA TGG^CCA 815 TGGCCA MscI 176 TGGCCA TGG^CCA 815 TGGCCA MspA1I 489 CCGCTG C[AC]G^C[GT]G 758 CAGCTG NlaIV 1095 GGGGCC GG[ACGT]^[ACGT]CC 1767 GGAGCC StyI 117 CCAAGG C^C[AT][AT]GG 309 CCAAGG TaqI 447 TCGA T^CGA 1228 TCGA Tsp45I 94 GTGAC ^GT[GC]AC 1799 GTCAC TthHB8I 447 TCGA T^CGA 1228 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BbvI BmyI BpmI BsiHKAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI