################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 25 CGCG CG^CG AlwNI 13 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 15 GGTCC G^G[AT]CC BamHI 410 GGATCC G^GATCC BfaI 389 CTAG C^TAG BsaI 55 GGTCTC GGTCTC(1/5) BsgI 10 GTGCAG GTGCAG(16/14) BsmAI 56 GTCTC GTCTC(1/5) BspMI 12 GCAGGT ACCTGC(4/8) BsrFI 43 ACCGGC [GA]^CCGG[CT] BsrI 352 ACTGG ACTGG(1/-1) BstUI 25 CGCG CG^CG BstYI 410 GGATCC [GA]^GATC[CT] Cac8I 26 GCGGGC GC[ACGT]^[ACGT]GC Cfr10I 43 ACCGGC [GA]^CCGG[CT] Cfr13I 15 GGTCC G^G[ACGT]CC DraII 14 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 48 CCACGG C^C[GA][CT]GG EcoO109I 14 AGGTCCT [GA]G^G[ACGT]CC[CT] FauI 26 GCGGG CCCGC(4/6) HapII 44 CCGG C^CGG HinfI 399 GATTC G^A[ACGT]TC HpaII 44 CCGG C^CGG MaeI 389 CTAG C^TAG MaeII 347 ACGT A^CGT MflI 410 GGATCC [GA]^GATC[CT] MspI 44 CCGG C^CGG MvnI 25 CGCG CG^CG MwoI 258 GCATCGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 240 CATG CATG^ PpuMI 14 AGGTCCT [GA]G^G[AT]CC[CT] PstI 31 CTGCAG CTGCA^G Sau96I 15 GGTCC G^G[ACGT]CC SfcI 31 CTGCAG C^T[GA][CT]AG TfiI 399 GATTC G^A[AT]TC XhoII 410 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 26 GCGG CCGC(-3/-1) 53 GCGG AcsI 319 AAATTC [GA]^AATT[CT] 340 AAATTC AfaI 107 GTAC GT^AC 373 GTAC ApoI 319 AAATTC [GA]^AATT[CT] 340 AAATTC BanI 243 GGTGCC G^G[CT][GA]CC 355 GGCACC BbsI 121 GAAGAC GAAGAC(2/6) 148 GAAGAC BpuAI 121 GAAGAC GAAGAC(2/6) 148 GAAGAC BsaJI 48 CCACGG C^C[ACGT][ACGT]GG 60 CCGAGG BstF5I 225 GGATG GGATG(2/0) 231 GGATG EaeI 45 CGGCCA [CT]^GGCC[GA] 168 TGGCCG FokI 225 GGATG GGATG(9/13) 231 GGATG HaeIII 46 GGCC GG^CC 169 GGCC HphI 41 TCACC GGTGA(8/7) 174 TCACC MaeIII 173 GTCAC ^GT[ACGT]AC 349 GTGAC RsaI 107 GTAC GT^AC 373 GTAC SfaNI 94 GATGC GCATC(5/9) 258 GCATC Tsp45I 173 GTCAC ^GT[GC]AC 349 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AlwI BbvI DpnII Fnu4HI ItaI MboI MboII MnlI NdeII NlaIV Sau3AI TseI Tsp509I