Restriction map for
pFN21AE6571
(Circular) (Six-base) MAPSORT of: pFN21AE6571.seq Check: 2828 from: 1 to: 5140
pFN21AE6571 5140 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2950 278
Size: 53 83 186 936 1414 2468
Fragments arranged by size:
2468 1414 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2483 1937
Size: 546 4594
Acc65I G'GTAC_C
Cuts at: 2461 2461
Size: 5140
AclI AA'CG_TT
Cuts at: 1563 3267 3640 1563
Size: 1704 373 3063
Fragments arranged by size:
3063 1704 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3203 4587 4855 1632
Size: 1571 1384 268 1917
Fragments arranged by size:
1917 1571 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4943
AflIII A'CryG_T
Cuts at: 1203 4039 5016 1203
Size: 2836 977 1327
Fragments arranged by size:
2836 1327 977
AgeI A'CCGG_T
Cuts at: 3971 3971
Size: 5140
AhdI GACnn_n'nnGTC
Cuts at: 3869 3869
Size: 5140
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5140
AlwNI CAG_nnn'CTG
Cuts at: 2023 2883 4455 2023
Size: 860 1572 2708
Fragments arranged by size:
2708 1572 860
ApaLI G'TGCA_C
Cuts at: 3197 4353 3197
Size: 1156 3984
ApoI r'AATT_y
Cuts at: 1493 2324 2345 2449 2675 2827 4936 1493
Size: 831 21 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 226 152 104 21
AseI AT'TA_AT
Cuts at: 160 1032 3694 160
Size: 872 2662 1606
Fragments arranged by size:
2662 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5140
AvaI C'yCGr_G
Cuts at: 1870 1943 2465 1870
Size: 73 522 4545
Fragments arranged by size:
4545 522 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2103 2136 2103
Size: 33 5107
BamHI G'GATC_C
Cuts at: 2415 2470 2837 2415
Size: 55 367 4718
Fragments arranged by size:
4718 367 55
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 2360 2461 3916 618
Size: 325 217 627 461 112 101 1455 1842
Fragments arranged by size:
1842 1455 627 461 325 217 112 101
BanII G_rGCy'C
Cuts at: 1391 2459 1391
Size: 1068 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 2160 928
Size: 971 234 27 3908
Fragments arranged by size:
3908 971 234 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3331 3365 1903
Size: 34 1394 34 3678
Fragments arranged by size:
3678 1394 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3032 4242 3032
Size: 1210 3930
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5140
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3751 136
Size: 107 122 71 631 605 2079 1525
Fragments arranged by size:
2079 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5135 5135
Size: 5140
BlpI GC'TnA_GC
Cuts at: 2557 2557
Size: 5140
Bme1580I G_kGCm'C
Cuts at: 1790 3201 4357 1790
Size: 1411 1156 2573
Fragments arranged by size:
2573 1411 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2871 3829 442
Size: 416 53 83 399 24 1454 958 1753
Fragments arranged by size:
1753 1454 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3800 1239
Size: 2561 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3265 4130 4428 3265
Size: 865 298 3977
Fragments arranged by size:
3977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 3803 882
Size: 617 567 1737 2219
Fragments arranged by size:
2219 1737 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 2931 493
Size: 711 1727 2702
Fragments arranged by size:
2702 1727 711
BsaBI GATnn'nnATC
Cuts at: 5134 5134
Size: 5140
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2947 3329 3964 275
Size: 53 83 186 936 1414 382 635 1451
Fragments arranged by size:
1451 1414 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3573 3971 4245 4392 4803 3573
Size: 398 274 147 411 3910
Fragments arranged by size:
3910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4915
Fragments arranged by size:
4915 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5140
BseYI C'CCAG_C
Cuts at: 4343 4839 4343
Size: 496 4644
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 1163
Size: 573 300 4267
Fragments arranged by size:
4267 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2590 3351 4379 1540
Size: 275 186 589 761 1028 2301
Fragments arranged by size:
2301 1028 761 589 275 186
BsiHKAI G_wGCw'C
Cuts at: 3201 3286 4357 3201
Size: 85 1071 3984
Fragments arranged by size:
3984 1071 85
BsmI GAATG_Cn'
Cuts at: 2651 2744 2651
Size: 93 5047
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2459 3201 3286 4357 1391
Size: 399 669 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 399 85
BspEI T'CCGG_A
Cuts at: 4803 4803
Size: 5140
BspHI T'CATG_A
Cuts at: 3029 4759 3029
Size: 1730 3410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2008 2495 844
Size: 1164 487 3489
Fragments arranged by size:
3489 1164 487
BsrBI CCG'CTC
Cuts at: 1394 1823 3027 1394
Size: 429 1204 3507
Fragments arranged by size:
3507 1204 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3635 3809 65
Size: 1183 2387 174 1396
Fragments arranged by size:
2387 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 3784 3971 1700
Size: 110 238 1736 187 2869
Fragments arranged by size:
2869 1736 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5140
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5140
BssSI C'ACGA_G
Cuts at: 3200 4212 3200
Size: 1012 4128
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5140
BstBI TT'CG_AA
Cuts at: 3944 3944
Size: 5140
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5140
BstYI r'GATC_y
Cuts at: 1972 2415 2470 2837 3222 3239 4691 4777 4789
Size: 443 55 367 385 17 1452 86 12
Cuts at: 4789 5135 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 443 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2053 513
Size: 552 939 49 3600
Fragments arranged by size:
3600 939 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4401
Fragments arranged by size:
4401 683 56
BtsI GCAGTG_nn'
Cuts at: 2656 3450 3470 2656
Size: 794 20 4326
Fragments arranged by size:
4326 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5105
DraI TTT'AAA
Cuts at: 2444 2444
Size: 5140
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4147 809
Size: 507 2831 1802
Fragments arranged by size:
2831 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2173 2587
Size: 54 1425 50 275 18 220 123 414
Cuts at: 2587 3476 5105 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 414 275 220 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2587 1537
Size: 275 775 4090
Fragments arranged by size:
4090 775 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3070 1353
Size: 1717 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3727 4101 4247 387
Size: 1064 138 2138 374 146 1280
Fragments arranged by size:
2138 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3203 3800 4587 4855 1239
Size: 393 1571 597 787 268 1524
Fragments arranged by size:
1571 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2020 2020
Size: 5140
EcoRI G'AATT_C
Cuts at: 2449 2827 2449
Size: 378 4762
FspI TGC'GCA
Cuts at: 3646 3646
Size: 5140
HaeII r_GCGC'y
Cuts at: 1678 4287 1678
Size: 2609 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2275 2307 3781 3813 3855
Size: 32 135 32 703 32 1474 32 42
Cuts at: 3855 3887 1373
Size: 32 2626
Fragments arranged by size:
2626 1474 703 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2484 2730 669
Size: 988 281 546 246 3079
Fragments arranged by size:
3079 988 546 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5140
HpaI GTT'AAC
Cuts at: 2730 2730
Size: 5140
KpnI G_GTAC'C
Cuts at: 2465 2465
Size: 5140
MfeI C'AATT_G
Cuts at: 2739 2739
Size: 5140
MluI A'CGCG_T
Cuts at: 5016 5016
Size: 5140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 4254 4438 1305
Size: 1016 1933 184 2007
Fragments arranged by size:
2007 1933 1016 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3318
Fragments arranged by size:
3318 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 3457 3616 518
Size: 648 1932 359 159 2042
Fragments arranged by size:
2042 1932 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2556 3233 4381 4626 1606
Size: 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3649
Fragments arranged by size:
3649 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5140
NotI GC'GGCC_GC
Cuts at: 2587 2587
Size: 5140
NspI r_CATG'y
Cuts at: 1132 1229 2498 4043 1132
Size: 97 1269 1545 2229
Fragments arranged by size:
2229 1545 1269 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5140
PciI A'CATG_T
Cuts at: 4039 4039
Size: 5140
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5140
PmeI GTTT'AAAC
Cuts at: 2444 2444
Size: 5140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 4722 4754 3209
Size: 32 1481 32 3595
Fragments arranged by size:
3595 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2020
Size: 5140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5140
PsiI TTA'TAA
Cuts at: 2710 2710
Size: 5140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2361 2393 2361
Size: 32 5108
PstI C_TGCA'G
Cuts at: 830 2040 2492 830
Size: 1210 452 3478
Fragments arranged by size:
3478 1210 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5140
SalI G'TCGA_C
Cuts at: 1936 2482 1936
Size: 546 4594
SbfI CC_TGCA'GG
Cuts at: 2492 2492
Size: 5140
ScaI AGT'ACT
Cuts at: 3388 5100 3388
Size: 1712 3428
SfcI C'TryA_G
Cuts at: 826 1045 2036 2488 3623 4304 4495 826
Size: 219 991 452 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 452 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5140
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5140
SmaI CCC'GGG
Cuts at: 1872 2467 1872
Size: 595 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3244 4145 4407 820
Size: 197 926 1301 901 262 1553
Fragments arranged by size:
1553 1301 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2931 493
Size: 2438 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5140
SphI G_CATG'C
Cuts at: 1132 2498 1132
Size: 1366 3774
SspI AAT'ATT
Cuts at: 5 52 3064 5
Size: 47 3012 2081
Fragments arranged by size:
3012 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5140
StyI C'CwwG_G
Cuts at: 513 1065 2004 2579 513
Size: 552 939 575 3074
Fragments arranged by size:
3074 939 575 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3167 3184 3337 3522 3967 325
Size: 1255 1587 17 153 185 445 1498
Fragments arranged by size:
1587 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3386 5098 96
Size: 274 80 33 51 1443 1409 1712 138
Fragments arranged by size:
1712 1443 1409 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2232 2264 5100 5132 2232
Size: 32 2836 32 2240
Fragments arranged by size:
2836 2240 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5140
XbaI T'CTAG_A
Cuts at: 2476 2476
Size: 5140
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5140
XmaI C'CCGG_G
Cuts at: 1870 2465 1870
Size: 595 4545
XmnI GAAnn'nnTTC
Cuts at: 3269 3269
Size: 5140
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2948 276
Size: 53 83 186 936 1414 2468
Fragments arranged by size:
2468 1414 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI