Restriction map for pFN21AB6827
 (Circular) (Six-base) MAPSORT of: pFN21AB6827.seq  Check: 8364  from: 1  to: 5203



pFN21AB6827  5203 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3013     278 
   Size:         53      83     186     936    1477    2468

  Fragments arranged by size: 

                2468    1477     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2546    1937 
   Size:        609    4594
 
Acc65I G'GTAC_C

Cuts at:   2524    2524 
   Size:       5203
 
AclI AA'CG_TT

Cuts at:   1563    3330    3703    1563 
   Size:       1767     373    3063

  Fragments arranged by size: 

                3063    1767     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3266    4650    4918    1632 
   Size:       1634    1384     268    1917

  Fragments arranged by size: 

                1917    1634    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2105     820 
   Size:        197    1088    3918

  Fragments arranged by size: 

                3918    1088     197
 
AflIII A'CryG_T

Cuts at:   1203    4102    5079    1203 
   Size:       2899     977    1327

  Fragments arranged by size: 

                2899    1327     977
 
AgeI A'CCGG_T

Cuts at:   4034    4034 
   Size:       5203
 
AhdI GACnn_n'nnGTC

Cuts at:   3932    3932 
   Size:       5203
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5203
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5203
 
AlwNI CAG_nnn'CTG

Cuts at:   2476    2946    4518    2476 
   Size:        470    1572    3161

  Fragments arranged by size: 

                3161    1572     470
 
ApaLI G'TGCA_C

Cuts at:   3260    4416    3260 
   Size:       1156    4047
 
ApoI r'AATT_y

Cuts at:   1493    2512    2738    2890    4999    1493 
   Size:       1019     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1019     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3757     160 
   Size:        872    2725    1606

  Fragments arranged by size: 

                2725    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5203
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2528    1870 
   Size:         73     585    4545

  Fragments arranged by size: 

                4545     585      73
 
BamHI G'GATC_C

Cuts at:   2533    2900    2533 
   Size:        367    4836
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2524    3979     618 
   Size:        325     217     627     737    1455    1842

  Fragments arranged by size: 

                1842    1455     737     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2522    1391 
   Size:       1131    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2304     928 
   Size:        971     405    3827

  Fragments arranged by size: 

                3827     971     405
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3394    3428    1903 
   Size:         34    1457      34    3678

  Fragments arranged by size: 

                3678    1457      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3095    4305    3095 
   Size:       1210    3993
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5203
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3814     136 
   Size:        107     122      71     631     605    2142    1525

  Fragments arranged by size: 

                2142    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5198    5198 
   Size:       5203
 
BlpI GC'TnA_GC

Cuts at:   2620    2620 
   Size:       5203
 
Bme1580I G_kGCm'C

Cuts at:   1790    3264    4420    1790 
   Size:       1474    1156    2573

  Fragments arranged by size: 

                2573    1474    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5203
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2934    3892     442 
   Size:        416      53      83     399      24    1517     958    1753

  Fragments arranged by size: 

                1753    1517     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5203
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3863    1239 
   Size:       2624    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3328    4193    4491    3328 
   Size:        865     298    4040

  Fragments arranged by size: 

                4040     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2279    3866     882 
   Size:        617     780    1587    2219

  Fragments arranged by size: 

                2219    1587     780     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2994     493 
   Size:        711    1790    2702

  Fragments arranged by size: 

                2702    1790     711
 
BsaBI GATnn'nnATC

Cuts at:   5197    5197 
   Size:       5203
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3010    3392    4027     275 
   Size:         53      83     186     936    1477     382     635    1451

  Fragments arranged by size: 

                1477    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3636    4034    4308    4455    4866    3636 
   Size:        398     274     147     411    3973

  Fragments arranged by size: 

                3973     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4978

  Fragments arranged by size: 

                4978     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2201    1186 
   Size:       1015    4188
 
BseYI C'CCAG_C

Cuts at:   4406    4902    4406 
   Size:        496    4707
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4630
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2653    3414    4442    1540 
   Size:        275     186     652     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     652     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3264    3349    4420    3264 
   Size:         85    1071    4047

  Fragments arranged by size: 

                4047    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2714    2807    2714 
   Size:         93    5110
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2522    3264    3349    4420    1391 
   Size:        399     732     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     732     399      85
 
BspEI T'CCGG_A

Cuts at:   4866    4866 
   Size:       5203
 
BspHI T'CATG_A

Cuts at:   3092    4822    3092 
   Size:       1730    3473
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2114    2558     844 
   Size:       1270     444    3489

  Fragments arranged by size: 

                3489    1270     444
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3090    1394 
   Size:        429    1267    3507

  Fragments arranged by size: 

                3507    1267     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3698    3872      65 
   Size:       1183    2450     174    1396

  Fragments arranged by size: 

                2450    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3847    4034    1700 
   Size:        110    2037     187    2869

  Fragments arranged by size: 

                2869    2037     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5203
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5203
 
BssSI C'ACGA_G

Cuts at:   3263    4275    3263 
   Size:       1012    4191
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5203
 
BstBI TT'CG_AA

Cuts at:   2349    4007    2349 
   Size:       1658    3545
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5203
 
BstYI r'GATC_y

Cuts at:   1972    2533    2900    3285    3302    4754    4840    4852    5198 
   Size:        561     367     385      17    1452      86      12     346

Cuts at:   5198    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     561     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4651
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4464

  Fragments arranged by size: 

                4464     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2719    3513    3533    2719 
   Size:        794      20    4389

  Fragments arranged by size: 

                4389     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5203
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5168
 
DraI TTT'AAA

Cuts at:   2245    2254    2507    2245 
   Size:          9     253    4941

  Fragments arranged by size: 

                4941     253       9
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2306    2384    4210     809 
   Size:        507     990      78    1826    1802

  Fragments arranged by size: 

                1826    1802     990     507      78
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2650    3539    5168 
   Size:         54    1425      50     275      18     820     889    1629

Cuts at:   5168       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     820     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2650    1537 
   Size:        275     838    4090

  Fragments arranged by size: 

                4090     838     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2485    3133    1353 
   Size:       1132     648    3423

  Fragments arranged by size: 

                3423    1132     648
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3790    4164    4310     387 
   Size:       1064     138    2201     374     146    1280

  Fragments arranged by size: 

                2201    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3266    3863    4650    4918    1239 
   Size:        393    1634     597     787     268    1524

  Fragments arranged by size: 

                1634    1524     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2077    2077 
   Size:       5203
 
EcoRI G'AATT_C

Cuts at:   2512    2890    2512 
   Size:        378    4825
 
FspI TGC'GCA

Cuts at:   3709    3709 
   Size:       5203
 
HaeII r_GCGC'y

Cuts at:   1678    4350    1678 
   Size:       2672    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3844    3876    3918    3950    1373 
   Size:         32     135      32    2272      32      42      32    2626

  Fragments arranged by size: 

                2626    2272     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2547    2793     669 
   Size:        988     281     609     246    3079

  Fragments arranged by size: 

                3079     988     609     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5203
 
HpaI GTT'AAC

Cuts at:   2793    2793 
   Size:       5203
 
KpnI G_GTAC'C

Cuts at:   2528    2528 
   Size:       5203
 
MfeI C'AATT_G

Cuts at:   2802    2802 
   Size:       5203
 
MluI A'CGCG_T

Cuts at:   5079    5079 
   Size:       5203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2413    4317    4501    1305 
   Size:       1108    1904     184    2007

  Fragments arranged by size: 

                2007    1904    1108     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3381

  Fragments arranged by size: 

                3381    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2133    2417    3161    3520    3679     518 
   Size:        648     967     284     744     359     159    2042

  Fragments arranged by size: 

                2042     967     744     648     359     284     159
 
MspA1I CmG'CkG

Cuts at:   1606    2619    3296    4444    4689    1606 
   Size:       1013     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1013     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4651
 
NdeI CA'TA_TG

Cuts at:    387    2083     387 
   Size:       1696    3507
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5203
 
NotI GC'GGCC_GC

Cuts at:   2650    2650 
   Size:       5203
 
NspI r_CATG'y

Cuts at:   1132    1229    2561    4106    1132 
   Size:         97    1332    1545    2229

  Fragments arranged by size: 

                2229    1545    1332      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5203
 
PciI A'CATG_T

Cuts at:   4102    4102 
   Size:       5203
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2422    1108 
   Size:       1314    3889
 
PmeI GTTT'AAAC

Cuts at:   2507    2507 
   Size:       5203
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3272    3304    4785    4817    3272 
   Size:         32    1481      32    3658

  Fragments arranged by size: 

                3658    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2077    2077 
   Size:       5203
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5203
 
PsiI TTA'TAA

Cuts at:   2773    2773 
   Size:       5203
 
PstI C_TGCA'G

Cuts at:    830    2555     830 
   Size:       1725    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5203
 
SalI G'TCGA_C

Cuts at:   1936    2545    1936 
   Size:        609    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2485    2485 
   Size:       5203
 
SbfI CC_TGCA'GG

Cuts at:   2555    2555 
   Size:       5203
 
ScaI AGT'ACT

Cuts at:   3451    5163    3451 
   Size:       1712    3491
 
SfcI C'TryA_G

Cuts at:    826    1045    2551    3686    4367    4558     826 
   Size:        219    1506    1135     681     191    1471

  Fragments arranged by size: 

                1506    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5203
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5203
 
SmaI CCC'GGG

Cuts at:   1872    2530    1872 
   Size:        658    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2105    3307    4208    4470     820 
   Size:        197     926     162    1202     901     262    1553

  Fragments arranged by size: 

                1553    1202     926     901     262     197     162
 
SnaBI TAC'GTA

Cuts at:    493    2994     493 
   Size:       2501    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5203
 
SphI G_CATG'C

Cuts at:   1132    2561    1132 
   Size:       1429    3774
 
SspI AAT'ATT

Cuts at:      5      52    2114    3127       5 
   Size:         47    2062    1013    2081

  Fragments arranged by size: 

                2081    2062    1013      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5203
 
StyI C'CwwG_G

Cuts at:    513    1065    2642     513 
   Size:        552    1577    3074

  Fragments arranged by size: 

                3074    1577     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3230    3247    3400    3585    4030     325 
   Size:       1255    1650      17     153     185     445    1498

  Fragments arranged by size: 

                1650    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3449    5161      96 
   Size:        274      80      33      51    1443    1472    1712     138

  Fragments arranged by size: 

                1712    1472    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5163    5195    5163 
   Size:         32    5171
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5203
 
XbaI T'CTAG_A

Cuts at:   2539    2539 
   Size:       5203
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5203
 
XmaI C'CCGG_G

Cuts at:   1870    2528    1870 
   Size:        658    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3332    3332 
   Size:       5203
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3011     276 
   Size:         53      83     186     936    1477    2468

  Fragments arranged by size: 

                2468    1477     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI     SalI     SapI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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