Restriction map for pFN21AB6149
 (Linear) (Six-base) MAPSORT of: KIBB6149.seq  Check: 764  from: 1  to: 1788



KIBB6149.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:30  ..

AccI GT'mk_AC

Cuts at:      0     185     536    1070    1788 
   Size:        185     351     534     718

  Fragments arranged by size: 

                 718     534     351     185
 
AclI AA'CG_TT

Cuts at:      0     848    1788 
   Size:        848     940
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    1371    1379    1676    1788 
   Size:        317    1054       8     297     112

  Fragments arranged by size: 

                1054     317     297     112       8
 
AflII C'TTAA_G

Cuts at:      0    1659    1788 
   Size:       1659     129
 
AlwNI CAG_nnn'CTG

Cuts at:      0     700    1788 
   Size:        700    1088
 
ApaI G_GGCC'C

Cuts at:      0      21    1788 
   Size:         21    1767
 
ApoI r'AATT_y

Cuts at:      0     489    1236    1750    1788 
   Size:        489     747     514      38

  Fragments arranged by size: 

                 747     514     489      38
 
AseI AT'TA_AT

Cuts at:      0     811    1788 
   Size:        811     977
 
BanII G_rGCy'C

Cuts at:      0      21      83    1788 
   Size:         21      62    1705

  Fragments arranged by size: 

                1705      62      21
 
BclI T'GATC_A

Cuts at:      0     906    1788 
   Size:        906     882
 
Bme1580I G_kGCm'C

Cuts at:      0      21     231    1788 
   Size:         21     210    1557

  Fragments arranged by size: 

                1557     210      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1598    1788 
   Size:       1598     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253    1432    1685    1788 
   Size:        253    1179     253     103

  Fragments arranged by size: 

                1179     253     253     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1322    1788 
   Size:       1322     466
 
BsaWI w'CCGG_w

Cuts at:      0     950    1788 
   Size:        950     838
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     251     281    1788 
   Size:        251      30    1507

  Fragments arranged by size: 

                1507     251      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1391    1788 
   Size:       1391     397
 
BseYI C'CCAG_C

Cuts at:      0     114    1788 
   Size:        114    1674
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    1788 
   Size:        826     962
 
BsiHKAI G_wGCw'C

Cuts at:      0      83    1788 
   Size:         83    1705
 
BsmI GAATG_Cn'

Cuts at:      0    1528    1707    1788 
   Size:       1528     179      81

  Fragments arranged by size: 

                1528     179      81
 
Bsp1286I G_dGCh'C

Cuts at:      0      21      83     231    1788 
   Size:         21      62     148    1557

  Fragments arranged by size: 

                1557     148      62      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65     848    1788 
   Size:         65     783     940

  Fragments arranged by size: 

                 940     783      65
 
BsrFI r'CCGG_y

Cuts at:      0    1268    1788 
   Size:       1268     520
 
BssHII G'CGCG_C

Cuts at:      0      26     110    1788 
   Size:         26      84    1678

  Fragments arranged by size: 

                1678      84      26
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      45    1788 
   Size:         45    1743
 
BstBI TT'CG_AA

Cuts at:      0     798    1788 
   Size:        798     990
 
BstEII G'GTnAC_C

Cuts at:      0     250    1788 
   Size:        250    1538
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     788    1788 
   Size:        788    1000
 
BstYI r'GATC_y

Cuts at:      0    1152    1362    1683    1788 
   Size:       1152     210     321     105

  Fragments arranged by size: 

                1152     321     210     105
 
BstZ17I GTA'TAC

Cuts at:      0     537    1788 
   Size:        537    1251
 
Bsu36I CC'TnA_GG

Cuts at:      0    1486    1788 
   Size:       1486     302
 
BtgI C'CryG_G

Cuts at:      0      21     398     938    1788 
   Size:         21     377     540     850

  Fragments arranged by size: 

                 850     540     377      21
 
BtsI GCAGTG_nn'

Cuts at:      0     326     607     766    1788 
   Size:        326     281     159    1022

  Fragments arranged by size: 

                1022     326     281     159
 
DraI TTT'AAA

Cuts at:      0     582    1319    1788 
   Size:        582     737     469

  Fragments arranged by size: 

                 737     582     469
 
EarI CTCTTCn'nnn_

Cuts at:      0     334     613    1022    1171    1258    1788 
   Size:        334     279     409     149      87     530

  Fragments arranged by size: 

                 530     409     334     279     149      87
 
EcoICRI GAG'CTC

Cuts at:      0      81    1788 
   Size:         81    1707
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     317    1371    1379    1432    1676    1685    1788 
   Size:        253      64    1054       8      53     244       9     103

  Fragments arranged by size: 

                1054     253     244     103      64      53       9       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     259    1788 
   Size:        259    1529
 
EcoO109I rG'GnC_Cy

Cuts at:      0      17     264    1664    1788 
   Size:         17     247    1400     124

  Fragments arranged by size: 

                1400     247     124      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299    1788 
   Size:        267      32    1489

  Fragments arranged by size: 

                1489     267      32
 
HindIII A'AGCT_T

Cuts at:      0    1589    1788 
   Size:       1589     199
 
MslI CAynn'nnrTG

Cuts at:      0     985    1788 
   Size:        985     803
 
MspA1I CmG'CkG

Cuts at:      0      23     101     152    1146    1739    1788 
   Size:         23      78      51     994     593      49

  Fragments arranged by size: 

                 994     593      78      51      49      23
 
NcoI C'CATG_G

Cuts at:      0     938    1788 
   Size:        938     850
 
NdeI CA'TA_TG

Cuts at:      0     564    1788 
   Size:        564    1224
 
NspI r_CATG'y

Cuts at:      0    1023    1602    1788 
   Size:       1023     579     186

  Fragments arranged by size: 

                1023     579     186
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276    1788 
   Size:        276    1512
 
PfoI T'CCnGG_A

Cuts at:      0     269    1409    1788 
   Size:        269    1140     379

  Fragments arranged by size: 

                1140     379     269
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1725    1757    1788 
   Size:       1725      32      31

  Fragments arranged by size: 

                1725      32      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     264    1788 
   Size:        264    1524
 
PspOMI G'GGCC_C

Cuts at:      0      17    1788 
   Size:         17    1771
 
PvuII CAG'CTG

Cuts at:      0     101     152    1146    1739    1788 
   Size:        101      51     994     593      49

  Fragments arranged by size: 

                 994     593     101      51      49
 
SacI G_AGCT'C

Cuts at:      0      83    1788 
   Size:         83    1705
 
SacII CC_GC'GG

Cuts at:      0      24    1788 
   Size:         24    1764
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1258    1788 
   Size:       1258     530
 
ScaI AGT'ACT

Cuts at:      0     440    1335    1788 
   Size:        440     895     453

  Fragments arranged by size: 

                 895     453     440
 
SfcI C'TryA_G

Cuts at:      0    1071    1788 
   Size:       1071     717
 
SmlI C'TyrA_G

Cuts at:      0    1337    1659    1788 
   Size:       1337     322     129

  Fragments arranged by size: 

                1337     322     129
 
StuI AGG'CCT

Cuts at:      0    1184    1484    1788 
   Size:       1184     300     304

  Fragments arranged by size: 

                1184     304     300
 
StyI C'CwwG_G

Cuts at:      0     189     787     938    1788 
   Size:        189     598     151     850

  Fragments arranged by size: 

                 850     598     189     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     677     852    1788 
   Size:        677     175     936

  Fragments arranged by size: 

                 936     677     175
 
TatI w'GTAC_w

Cuts at:      0     438    1031    1333    1775    1788 
   Size:        438     593     302     442      13

  Fragments arranged by size: 

                 593     442     438     302      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     937     969    1788 
   Size:        937      32     819

  Fragments arranged by size: 

                 937     819      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     284    1788 
   Size:        284    1504
 
XbaI T'CTAG_A

Cuts at:      0    1206    1788 
   Size:       1206     582
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     196     396    1788 
   Size:        196     200    1392

  Fragments arranged by size: 

                1392     200     196
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII    AlwNI     ApaI     ApoI     AseI
    BanII     BclI Bme1580I     BmrI     BpmI    BpuEI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     DraI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    Hin4I  HindIII     MslI   MspA1I     NcoI     NdeI     NspI    PflMI
     PfoI     PpiI    PpuMI   PspOMI    PvuII     SacI    SacII     SapI
     ScaI     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI    BssSI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XhoI     XmaI     XmnI
     ZraI
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