################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 743 GTCGAC GT^[AC][GT]AC AcyI 965 GGCGCC G[GA]^CG[CT]C AlwI 791 GGATC GGATC(4/5) AocI 802 CCTGAGG CC^T[ACGT]AGG ApaI 91 GGGCCC GGGCC^C AvaI 668 CCCGGG C^[CT]CG[GA]G AvrII 543 CCTAGG C^CTAGG BbeI 965 GGCGCC GGCGC^C BlnI 543 CCTAGG C^CTAGG BsaHI 965 GGCGCC G[GA]^CG[CT]C BsoBI 668 CCCGGG C^[CT]CG[GA]G BspMI 503 ACCTGC ACCTGC(4/8) BsrDI 11 CATTGC GCAATG(2/0) BstAPI 710 GCATCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 802 CCTGAGG CC^T[ACGT]AGG DrdI 376 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 802 CCTGAGG CC^T[ACGT]AGG EcoNI 504 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 117 GCGGG CCCGC(4/6) Hin1I 965 GGCGCC G[GA]^CG[CT]C HincII 743 GTCGAC GT[CT]^[GA]AC HindII 743 GTCGAC GT[CT]^[GA]AC KasI 965 GGCGCC G^GCGCC MaeIII 374 GTGAC ^GT[ACGT]AC MboII 900 TCTTC GAAGA(8/7) MslI 885 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 965 GGCGCC GG^CGCC PpuMI 242 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 917 CAGCTG CAG^CTG SacI 621 GAGCTC GAGCT^C SalI 743 GTCGAC G^TCGAC SfoI 965 GGCGCC GGC^GCC SmaI 668 CCCGGG CCC^GGG SphI 857 GCATGC GCATG^C TfiI 696 GATTC G^A[AT]TC Tsp45I 374 GTGAC ^GT[GC]AC XbaI 261 TCTAGA T^CTAGA XmaCI 668 CCCGGG C^CCGGG XmaI 668 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwNI 499 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 522 CAGTGGCTG AspHI 621 GAGCTC G[AT]GC[AT]^C 892 GTGCTC BalI 50 TGGCCA TGG^CCA 137 TGGCCA BfaI 262 CTAG C^TAG 544 CTAG BsiHKAI 621 GAGCTC G[AT]GC[AT]^C 892 GTGCTC BsmFI 995 GGGAC GGGAC(10/14) 244 GTCCC CfoI 177 GCGC GCG^C 966 GCGC DpnII 133 GATC ^GATC 792 GATC DsaI 237 CCACGG C^C[GA][CT]GG 956 CCACGG EaeI 50 TGGCCA [CT]^GGCC[GA] 137 TGGCCA HaeII 176 AGCGCC [GA]GCGC^[CT] 965 GGCGCC HapII 669 CCGG C^CGG 963 CCGG HhaI 177 GCGC GCG^C 966 GCGC HinP1I 177 GCGC G^CGC 966 GCGC HpaII 669 CCGG C^CGG 963 CCGG MaeI 262 CTAG C^TAG 544 CTAG MboI 133 GATC ^GATC 792 GATC MluNI 50 TGGCCA TGG^CCA 137 TGGCCA MscI 50 TGGCCA TGG^CCA 137 TGGCCA MspA1I 115 CAGCGG C[AC]G^C[GT]G 917 CAGCTG MspI 669 CCGG C^CGG 963 CCGG NdeII 133 GATC ^GATC 792 GATC NspI 82 GCATGT [GA]CATG^[CT] 857 GCATGC PflMI 79 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 521 CCAGTGGCTGG PleI 333 GACTC GAGTC(4/5) 452 GAGTC Sau3AI 133 GATC ^GATC 792 GATC SmlI 557 CTCAAG C^T[CT][GA]AG 904 CTCAAG TaqI 713 TCGA T^CGA 744 TCGA TthHB8I 713 TCGA T^CGA 744 TCGA Van91I 79 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 521 CCAGTGGCTGG XcmI 95 CCACCTTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 479 CCAACCTCACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MnlI MvaI MwoI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI StuI StyI TseI TspRI