(Circular) (Six-base) MAPSORT of: pFN21AE1834.seq Check: 3866 from: 1 to: 5761 pFN21AE1834 5761 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2749 3104 1937 Size: 812 355 4594 Fragments arranged by size: 4594 812 355 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 3824 5208 5476 1632 Size: 945 1247 1384 268 1917 Fragments arranged by size: 1917 1384 1247 945 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2894 1166 Size: 1728 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2509 2532 3504 5076 2509 Size: 23 972 1572 3194 Fragments arranged by size: 3194 1572 972 23 ApaI G_GGCC'C Cuts at: 2100 2100 Size: 5761 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2673 3086 1870 Size: 73 730 413 4545 Fragments arranged by size: 4545 730 413 73 AvrII C'CTAG_G Cuts at: 2548 2548 Size: 5761 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2583 2930 2970 3082 4537 Size: 325 217 627 796 347 40 112 1455 Cuts at: 4537 618 Size: 1842 Fragments arranged by size: 1842 1455 796 627 347 325 217 112 40 BanII G_rGCy'C Cuts at: 1391 2100 2630 2948 3080 1391 Size: 709 530 318 132 4072 Fragments arranged by size: 4072 709 530 318 132 BbeI G_GCGC'C Cuts at: 2974 2974 Size: 5761 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 2100 2263 2586 2846 3822 4978 1790 Size: 310 163 323 260 976 1156 2573 Fragments arranged by size: 2573 1156 976 323 310 260 163 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2534 2615 2958 4421 1239 Size: 893 402 81 343 1463 2579 Fragments arranged by size: 2579 1463 893 402 343 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2547 2894 3886 4751 5049 2547 Size: 347 992 865 298 3259 Fragments arranged by size: 3259 992 865 347 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2971 3568 3950 4585 Size: 53 83 186 936 1438 597 382 635 Cuts at: 4585 275 Size: 1451 Fragments arranged by size: 1451 1438 936 635 597 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2002 2024 2350 1186 Size: 816 22 326 4597 Fragments arranged by size: 4597 816 326 22 BseYI C'CCAG_C Cuts at: 2178 2467 4964 5460 2178 Size: 289 2497 496 2479 Fragments arranged by size: 2497 2479 496 289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2211 2438 3076 1163 Size: 573 417 58 227 638 3848 Fragments arranged by size: 3848 638 573 417 227 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2630 2901 3822 3907 4978 2630 Size: 271 921 85 1071 3413 Fragments arranged by size: 3413 1071 921 271 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2263 2586 2630 2846 2901 2948 Size: 399 310 163 323 44 216 55 47 Cuts at: 2948 3080 3822 3907 4978 1391 Size: 132 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 323 310 216 163 132 85 55 47 44 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3116 844 Size: 1673 599 3489 Fragments arranged by size: 3489 1673 599 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2015 4256 4430 65 Size: 1183 767 2241 174 1396 Fragments arranged by size: 2241 1396 1183 767 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 2721 794 Size: 1927 3834 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756 Size: 1119 367 385 17 1452 86 12 346 Cuts at: 5756 1972 Size: 1977 Fragments arranged by size: 1977 1452 1119 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2808 2808 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2242 2961 513 Size: 552 1177 719 3313 Fragments arranged by size: 3313 1177 719 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2228 2846 2889 2901 3064 3277 4071 4091 2228 Size: 618 43 12 163 213 794 20 3898 Fragments arranged by size: 3898 794 618 213 163 43 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2034 2069 529 Size: 35 1470 35 4221 Fragments arranged by size: 4221 1470 35 35 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2387 4768 809 Size: 507 1071 2381 1802 Fragments arranged by size: 2381 1802 1071 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2142 3208 Size: 54 1425 50 275 18 225 87 1066 Cuts at: 3208 4097 5726 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1066 889 275 225 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2628 2628 Size: 5761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2534 2577 2615 2958 3824 4421 Size: 393 500 402 43 38 343 866 597 Cuts at: 4421 5208 5476 1239 Size: 787 268 1524 Fragments arranged by size: 1524 866 787 597 500 402 393 343 268 43 38 EcoNI CCTnn'n_nnAGG Cuts at: 2513 2513 Size: 5761 EcoO109I rG'GnC_Cy Cuts at: 2096 2248 2552 2096 Size: 152 304 5305 Fragments arranged by size: 5305 304 152 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2185 2974 4908 1678 Size: 507 789 1934 2531 Fragments arranged by size: 2531 1934 789 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2700 2731 2732 2763 4402 Size: 32 135 32 1128 31 1 31 1639 Cuts at: 4402 4434 4476 4508 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1639 1128 135 42 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2750 3105 3351 669 Size: 988 281 812 355 246 3079 Fragments arranged by size: 3079 988 812 355 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KasI G'GCGC_C Cuts at: 2970 2970 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2069 4875 5059 1305 Size: 764 2806 184 2007 Fragments arranged by size: 2806 2007 764 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2144 10 Size: 54 1425 343 225 87 3627 Fragments arranged by size: 3627 1425 343 225 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2894 3719 4078 4237 518 Size: 648 1728 825 359 159 2042 Fragments arranged by size: 2042 1728 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2924 3177 3854 5002 5247 1606 Size: 516 802 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 802 677 516 253 245 NarI GG'CG_CC Cuts at: 2971 2971 Size: 5761 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5209 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2091 2866 3119 4664 1132 Size: 97 862 775 253 1545 2229 Fragments arranged by size: 2229 1545 862 775 253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 2090 2532 1108 Size: 982 442 4337 Fragments arranged by size: 4337 982 442 PfoI T'CCnGG_A Cuts at: 2735 2735 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2248 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PspOMI G'GGCC_C Cuts at: 2096 2096 Size: 5761 PstI C_TGCA'G Cuts at: 830 2235 2576 2896 2929 3113 830 Size: 1405 341 320 33 184 3478 Fragments arranged by size: 3478 1405 341 320 184 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2924 2924 Size: 5761 SacI G_AGCT'C Cuts at: 2630 2630 Size: 5761 SalI G'TCGA_C Cuts at: 1936 2748 3103 1936 Size: 812 355 4594 Fragments arranged by size: 4594 812 355 SanDI GG'GwC_CC Cuts at: 2248 2248 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2231 2572 2892 2925 3109 4244 4925 Size: 219 1186 341 320 33 184 1135 681 Cuts at: 4925 5116 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 681 341 320 219 191 184 33 SfoI GGC'GCC Cuts at: 2972 2972 Size: 5761 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 2675 3088 1872 Size: 803 413 4545 Fragments arranged by size: 4545 803 413 SmlI C'TyrA_G Cuts at: 820 1017 1943 2562 2909 3865 4766 5028 820 Size: 197 926 619 347 956 901 262 1553 Fragments arranged by size: 1553 956 926 901 619 347 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 2866 3119 1132 Size: 1734 253 3774 Fragments arranged by size: 3774 1734 253 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 2319 2659 3047 1517 Size: 802 340 388 4231 Fragments arranged by size: 4231 802 388 340 StyI C'CwwG_G Cuts at: 513 1065 2103 2493 2548 2725 3200 513 Size: 552 1038 390 55 177 475 3074 Fragments arranged by size: 3074 1038 552 475 390 177 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 2630 3042 3788 3805 3958 4143 Size: 1255 612 438 412 746 17 153 185 Cuts at: 4143 4588 325 Size: 445 1498 Fragments arranged by size: 1498 1255 746 612 445 438 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4007 5719 96 Size: 274 80 33 51 1443 2030 1712 138 Fragments arranged by size: 2030 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 2266 3097 2266 Size: 831 4930 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2491 2107 Size: 384 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2673 3086 1870 Size: 803 413 4545 Fragments arranged by size: 4545 803 413 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI