(Linear) (Six-base) MAPSORT of: KIEE2289.seq Check: 3901 from: 1 to: 1524 KIEE2289.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AccI GT'mk_AC Cuts at: 0 698 1022 1319 1340 1524 Size: 698 324 297 21 184 Fragments arranged by size: 698 324 297 184 21 Acc65I G'GTAC_C Cuts at: 0 107 1524 Size: 107 1417 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1215 1524 Size: 1215 309 AhdI GACnn_n'nnGTC Cuts at: 0 93 1524 Size: 93 1431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 464 1524 Size: 464 1060 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 334 366 1524 Size: 334 32 1158 Fragments arranged by size: 1158 334 32 AlwNI CAG_nnn'CTG Cuts at: 0 15 1524 Size: 15 1509 ApaI G_GGCC'C Cuts at: 0 234 899 964 1524 Size: 234 665 65 560 Fragments arranged by size: 665 560 234 65 ApaLI G'TGCA_C Cuts at: 0 562 1524 Size: 562 962 AseI AT'TA_AT Cuts at: 0 1082 1524 Size: 1082 442 AvaI C'yCGr_G Cuts at: 0 374 1524 Size: 374 1150 BamHI G'GATC_C Cuts at: 0 264 749 1524 Size: 264 485 775 Fragments arranged by size: 775 485 264 BanI G'GyrC_C Cuts at: 0 78 107 1346 1524 Size: 78 29 1239 178 Fragments arranged by size: 1239 178 78 29 BanII G_rGCy'C Cuts at: 0 234 899 964 998 1192 1524 Size: 234 665 65 34 194 332 Fragments arranged by size: 665 332 234 194 65 34 BbeI G_GCGC'C Cuts at: 0 82 1350 1524 Size: 82 1268 174 Fragments arranged by size: 1268 174 82 BbsI GAAGACnn'nnnn_ Cuts at: 0 286 581 913 1524 Size: 286 295 332 611 Fragments arranged by size: 611 332 295 286 BciVI GTATCCnnnnn_n' Cuts at: 0 1479 1524 Size: 1479 45 BglI GCCn_nnn'nGGC Cuts at: 0 229 688 1524 Size: 229 459 836 Fragments arranged by size: 836 459 229 Bme1580I G_kGCm'C Cuts at: 0 234 566 665 827 836 899 964 1524 Size: 234 332 99 162 9 63 65 560 Fragments arranged by size: 560 332 234 162 99 65 63 9 BmrI ACTGGGnnnn_n' Cuts at: 0 688 823 839 1524 Size: 688 135 16 685 Fragments arranged by size: 688 685 135 16 BmtI G_CTAG'C Cuts at: 0 1219 1524 Size: 1219 305 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 263 1524 Size: 263 1261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 426 1524 Size: 330 96 1098 Fragments arranged by size: 1098 330 96 BsaHI Gr'CG_yC Cuts at: 0 79 1347 1524 Size: 79 1268 177 Fragments arranged by size: 1268 177 79 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 334 364 1052 1082 1524 Size: 334 30 688 30 442 Fragments arranged by size: 688 442 334 30 30 BseYI C'CCAG_C Cuts at: 0 21 300 552 891 1524 Size: 21 279 252 339 633 Fragments arranged by size: 633 339 279 252 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 712 1524 Size: 460 252 812 Fragments arranged by size: 812 460 252 BsiHKAI G_wGCw'C Cuts at: 0 566 1524 Size: 566 958 Bsp1286I G_dGCh'C Cuts at: 0 234 566 665 827 836 899 964 998 Size: 234 332 99 162 9 63 65 34 Cuts at: 998 1192 1524 Size: 194 332 Fragments arranged by size: 332 332 234 194 162 99 65 63 34 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 353 623 1524 Size: 353 270 901 Fragments arranged by size: 901 353 270 BsrDI GCAATG_nn' Cuts at: 0 417 623 1524 Size: 417 206 901 Fragments arranged by size: 901 417 206 BsrFI r'CCGG_y Cuts at: 0 710 1524 Size: 710 814 BstXI CCAn_nnnn'nTGG Cuts at: 0 891 1524 Size: 891 633 BstYI r'GATC_y Cuts at: 0 130 264 749 1524 Size: 130 134 485 775 Fragments arranged by size: 775 485 134 130 Bsu36I CC'TnA_GG Cuts at: 0 1464 1524 Size: 1464 60 BtgI C'CryG_G Cuts at: 0 83 474 603 900 1524 Size: 83 391 129 297 624 Fragments arranged by size: 624 391 297 129 83 BtsI GCAGTG_nn' Cuts at: 0 564 1210 1524 Size: 564 646 314 Fragments arranged by size: 646 564 314 EaeI y'GGCC_r Cuts at: 0 477 1311 1524 Size: 477 834 213 Fragments arranged by size: 834 477 213 EarI CTCTTCn'nnn_ Cuts at: 0 766 797 1524 Size: 766 31 727 Fragments arranged by size: 766 727 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 1524 Size: 451 1073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 426 1215 1524 Size: 330 96 789 309 Fragments arranged by size: 789 330 309 96 EcoNI CCTnn'n_nnAGG Cuts at: 0 125 988 1183 1524 Size: 125 863 195 341 Fragments arranged by size: 863 341 195 125 EcoO109I rG'GnC_Cy Cuts at: 0 57 230 596 681 895 896 961 1524 Size: 57 173 366 85 214 1 65 563 Fragments arranged by size: 563 366 214 173 85 65 57 1 EcoRV GAT'ATC Cuts at: 0 156 1524 Size: 156 1368 FspI TGC'GCA Cuts at: 0 619 1524 Size: 619 905 HaeII r_GCGC'y Cuts at: 0 82 1077 1350 1524 Size: 82 995 273 174 Fragments arranged by size: 995 273 174 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 741 773 1398 1430 1524 Size: 79 32 630 32 625 32 94 Fragments arranged by size: 630 625 94 79 32 32 32 KasI G'GCGC_C Cuts at: 0 78 1346 1524 Size: 78 1268 178 Fragments arranged by size: 1268 178 78 KpnI G_GTAC'C Cuts at: 0 111 1524 Size: 111 1413 MslI CAynn'nnrTG Cuts at: 0 734 1524 Size: 734 790 MspA1I CmG'CkG Cuts at: 0 15 213 308 1524 Size: 15 198 95 1216 Fragments arranged by size: 1216 198 95 15 NarI GG'CG_CC Cuts at: 0 79 1347 1524 Size: 79 1268 177 Fragments arranged by size: 1268 177 79 NcoI C'CATG_G Cuts at: 0 474 1524 Size: 474 1050 NheI G'CTAG_C Cuts at: 0 1215 1524 Size: 1215 309 PflMI CCAn_nnn'nTGG Cuts at: 0 639 651 1447 1524 Size: 639 12 796 77 Fragments arranged by size: 796 639 77 12 PfoI T'CCnGG_A Cuts at: 0 398 1524 Size: 398 1126 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 334 366 1524 Size: 334 32 1158 Fragments arranged by size: 1158 334 32 PpuMI rG'GwC_Cy Cuts at: 0 57 596 1524 Size: 57 539 928 Fragments arranged by size: 928 539 57 PspOMI G'GGCC_C Cuts at: 0 230 895 960 1524 Size: 230 665 65 564 Fragments arranged by size: 665 564 230 65 PvuII CAG'CTG Cuts at: 0 15 213 1524 Size: 15 198 1311 Fragments arranged by size: 1311 198 15 SfcI C'TryA_G Cuts at: 0 112 1427 1524 Size: 112 1315 97 Fragments arranged by size: 1315 112 97 SfiI GGCCn_nnn'nGGCC Cuts at: 0 688 1524 Size: 688 836 SfoI GGC'GCC Cuts at: 0 80 1348 1524 Size: 80 1268 176 Fragments arranged by size: 1268 176 80 SmaI CCC'GGG Cuts at: 0 376 1524 Size: 376 1148 SrfI GCCC'GGGC Cuts at: 0 376 1524 Size: 376 1148 SspI AAT'ATT Cuts at: 0 1392 1524 Size: 1392 132 StyI C'CwwG_G Cuts at: 0 474 1481 1524 Size: 474 1007 43 Fragments arranged by size: 1007 474 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 746 1524 Size: 746 778 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 588 620 919 951 1052 1084 1524 Size: 588 32 299 32 101 32 440 Fragments arranged by size: 588 440 299 101 32 32 32 XbaI T'CTAG_A Cuts at: 0 123 1524 Size: 123 1401 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 760 1524 Size: 760 764 XmaI C'CCGG_G Cuts at: 0 374 1524 Size: 374 1150 XmnI GAAnn'nnTTC Cuts at: 0 1392 1524 Size: 1392 132 Enzymes that do cut: AccI Acc65I AcuI AhdI AlfI AloI AlwNI ApaI ApaLI AseI AvaI BamHI BanI BanII BbeI BbsI BciVI BglI Bme1580I BmrI BmtI BplI BpmI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI KpnI MslI MspA1I NarI NcoI NheI PflMI PfoI PpiI PpuMI PspOMI PvuII SfcI SfiI SfoI SmaI SrfI SspI StyI TaqII TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AleI ApoI AscI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI StuI SwaI TatI Tth111I XhoI ZraI