(Circular) (Six-base) MAPSORT of: pFN21AE3873.seq Check: 7108 from: 1 to: 6157 pFN21AE3873 6157 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 3967 278 Size: 53 83 186 936 561 1870 2468 Fragments arranged by size: 2468 1870 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 3500 1937 Size: 1563 4594 Acc65I G'GTAC_C Cuts at: 3478 3478 Size: 6157 AclI AA'CG_TT Cuts at: 1563 4284 4657 1563 Size: 2721 373 3063 Fragments arranged by size: 3063 2721 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3125 4220 5604 5872 1632 Size: 1493 1095 1384 268 1917 Fragments arranged by size: 1917 1493 1384 1095 268 AfeI AGC'GCT Cuts at: 2723 2723 Size: 6157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5960 AflIII A'CryG_T Cuts at: 1203 2503 5056 6033 1203 Size: 1300 2553 977 1327 Fragments arranged by size: 2553 1327 1300 977 AgeI A'CCGG_T Cuts at: 4988 4988 Size: 6157 AhdI GACnn_n'nnGTC Cuts at: 4886 4886 Size: 6157 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6157 AlwNI CAG_nnn'CTG Cuts at: 2214 2374 3098 3154 3157 3310 3900 5472 2214 Size: 160 724 56 3 153 590 1572 2899 Fragments arranged by size: 2899 1572 724 590 160 153 56 3 ApaLI G'TGCA_C Cuts at: 4214 5370 4214 Size: 1156 5001 ApoI r'AATT_y Cuts at: 1493 3466 3692 3844 5953 1493 Size: 1973 226 152 2109 1697 Fragments arranged by size: 2109 1973 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4711 160 Size: 872 3679 1606 Fragments arranged by size: 3679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2649 2922 3133 3337 3482 1870 Size: 73 84 622 273 211 204 145 4545 Fragments arranged by size: 4545 622 273 211 204 145 84 73 BamHI G'GATC_C Cuts at: 3487 3854 3487 Size: 367 5790 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2524 3478 4933 618 Size: 325 217 627 737 954 1455 1842 Fragments arranged by size: 1842 1455 954 737 627 325 217 BanII G_rGCy'C Cuts at: 1391 2252 3233 3476 1391 Size: 861 981 243 4072 Fragments arranged by size: 4072 981 861 243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4348 4382 1903 Size: 34 2411 34 3678 Fragments arranged by size: 3678 2411 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4049 5259 4049 Size: 1210 4947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4768 136 Size: 107 122 71 631 605 3096 1525 Fragments arranged by size: 3096 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2414 6152 2414 Size: 3738 2419 BlpI GC'TnA_GC Cuts at: 2148 3574 2148 Size: 1426 4731 Bme1580I G_kGCm'C Cuts at: 1790 2028 2125 2240 2529 2990 4218 5374 1790 Size: 238 97 115 289 461 1228 1156 2573 Fragments arranged by size: 2573 1228 1156 461 289 238 115 97 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 4954 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2234 3117 3888 Size: 416 53 83 399 24 817 883 771 Cuts at: 3888 4846 442 Size: 958 1753 Fragments arranged by size: 1753 958 883 817 771 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6157 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2163 2743 3196 2163 Size: 580 453 5124 Fragments arranged by size: 5124 580 453 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2274 2396 2722 2888 3050 3083 3299 Size: 884 151 122 326 166 162 33 216 Cuts at: 3299 4817 1239 Size: 1518 2579 Fragments arranged by size: 2579 1518 884 326 216 166 162 151 122 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 4282 5147 5445 3209 Size: 1073 865 298 3921 Fragments arranged by size: 3921 1073 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4820 882 Size: 617 3321 2219 Fragments arranged by size: 3321 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2023 3948 493 Size: 711 819 1925 2702 Fragments arranged by size: 2702 1925 819 711 BsaBI GATnn'nnATC Cuts at: 2642 6151 2642 Size: 3509 2648 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 3964 4346 4981 Size: 53 83 186 936 561 1870 382 635 Cuts at: 4981 275 Size: 1451 Fragments arranged by size: 1870 1451 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2992 3414 4590 4988 5262 5409 5820 2992 Size: 422 1176 398 274 147 411 3329 Fragments arranged by size: 3329 1176 422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 1178 Size: 30 165 30 1038 30 4864 Fragments arranged by size: 4864 1038 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2246 2716 3188 3296 1186 Size: 934 126 470 472 108 4047 Fragments arranged by size: 4047 934 472 470 126 108 BseYI C'CCAG_C Cuts at: 2360 2367 2833 3091 3157 5360 5856 2360 Size: 7 466 258 66 2203 496 2661 Fragments arranged by size: 2661 2203 496 466 258 66 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2774 1163 Size: 573 531 507 4546 Fragments arranged by size: 4546 573 531 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3607 4368 5396 1540 Size: 275 186 1606 761 1028 2301 Fragments arranged by size: 2301 1606 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 3048 3180 3233 3402 4218 4303 5374 2691 Size: 357 132 53 169 816 85 1071 3474 Fragments arranged by size: 3474 1071 816 357 169 132 85 53 BsmI GAATG_Cn' Cuts at: 3668 3761 3668 Size: 93 6064 BsmBI CGTCTCn'nnnn_ Cuts at: 2101 2915 2101 Size: 814 5343 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2125 2240 2252 2529 2691 2990 Size: 399 238 97 115 12 277 162 299 Cuts at: 2990 3048 3180 3233 3402 3476 4218 4303 5374 Size: 58 132 53 169 74 742 85 1071 Cuts at: 5374 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 299 277 238 169 162 132 115 97 85 74 58 53 12 BspEI T'CCGG_A Cuts at: 5820 5820 Size: 6157 BspHI T'CATG_A Cuts at: 4046 5776 4046 Size: 1730 4427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3512 844 Size: 2668 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2736 3193 4044 1394 Size: 429 913 457 851 3507 Fragments arranged by size: 3507 913 851 457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 2742 4652 4826 65 Size: 1183 1063 431 1910 174 1396 Fragments arranged by size: 1910 1396 1183 1063 431 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3375 3432 4801 4988 1700 Size: 110 1565 57 1369 187 2869 Fragments arranged by size: 2869 1565 1369 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6157 BssSI C'ACGA_G Cuts at: 4217 5229 4217 Size: 1012 5145 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6157 BstBI TT'CG_AA Cuts at: 4961 4961 Size: 6157 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6157 BstXI CCAn_nnnn'nTGG Cuts at: 2252 2252 Size: 6157 BstYI r'GATC_y Cuts at: 1972 2414 2637 3251 3487 3854 4239 4256 5708 Size: 442 223 614 236 367 385 17 1452 Cuts at: 5708 5794 5806 6152 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 614 442 385 367 346 236 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5605 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 487 Size: 683 56 1106 4312 Fragments arranged by size: 4312 1106 683 56 BtsI GCAGTG_nn' Cuts at: 3673 4467 4487 3673 Size: 794 20 5343 Fragments arranged by size: 5343 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6157 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6122 DraI TTT'AAA Cuts at: 3461 3461 Size: 6157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2093 3114 5164 809 Size: 507 777 1021 2050 1802 Fragments arranged by size: 2050 1802 1021 777 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2784 2844 3604 Size: 54 1425 50 275 18 954 60 760 Cuts at: 3604 4493 6122 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 954 889 760 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3604 1537 Size: 275 1792 4090 Fragments arranged by size: 4090 1792 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 4087 1353 Size: 1256 1478 3423 Fragments arranged by size: 3423 1478 1256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2454 2513 4744 5118 5264 387 Size: 1064 138 865 59 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 865 374 146 138 59 EcoICRI GAG'CTC Cuts at: 3231 3231 Size: 6157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2274 2396 2722 2888 3050 3083 Size: 393 491 151 122 326 166 162 33 Cuts at: 3083 3125 3299 4220 4817 5604 5872 1239 Size: 42 174 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 597 491 393 326 268 174 166 162 151 122 42 33 EcoNI CCTnn'n_nnAGG Cuts at: 2105 3281 2105 Size: 1176 4981 EcoO109I rG'GnC_Cy Cuts at: 3116 3307 3116 Size: 191 5966 EcoRI G'AATT_C Cuts at: 3466 3844 3466 Size: 378 5779 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2620 3395 2620 Size: 775 5382 FspI TGC'GCA Cuts at: 4663 4663 Size: 6157 HaeII r_GCGC'y Cuts at: 1678 2374 2725 5304 1678 Size: 696 351 2579 2531 Fragments arranged by size: 2579 2531 696 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2673 2705 4798 4830 4872 Size: 32 135 32 1101 32 2093 32 42 Cuts at: 4872 4904 1373 Size: 32 2626 Fragments arranged by size: 2626 2093 1101 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 3501 3747 669 Size: 988 281 322 1241 246 3079 Fragments arranged by size: 3079 1241 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6157 HpaI GTT'AAC Cuts at: 3747 3747 Size: 6157 KpnI G_GTAC'C Cuts at: 3482 3482 Size: 6157 MfeI C'AATT_G Cuts at: 3756 3756 Size: 6157 MluI A'CGCG_T Cuts at: 6033 6033 Size: 6157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2790 5271 5455 1305 Size: 1485 2481 184 2007 Fragments arranged by size: 2481 2007 1485 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 2846 10 Size: 54 1425 343 954 60 3321 Fragments arranged by size: 3321 1425 954 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 4115 4474 4633 518 Size: 648 1419 1530 359 159 2042 Fragments arranged by size: 2042 1530 1419 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2842 3028 3154 3573 4250 5398 5643 1606 Size: 1236 186 126 419 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 677 419 245 186 126 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5605 NdeI CA'TA_TG Cuts at: 387 2515 2968 387 Size: 2128 453 3576 Fragments arranged by size: 3576 2128 453 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6157 NotI GC'GGCC_GC Cuts at: 3604 3604 Size: 6157 NspI r_CATG'y Cuts at: 1132 1229 2507 3515 5060 1132 Size: 97 1278 1008 1545 2229 Fragments arranged by size: 2229 1545 1278 1008 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6157 PciI A'CATG_T Cuts at: 2503 5056 2503 Size: 2553 3604 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 3098 1108 Size: 1266 724 4167 Fragments arranged by size: 4167 1266 724 PmeI GTTT'AAAC Cuts at: 3461 3461 Size: 6157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4226 4258 5739 5771 4226 Size: 32 1481 32 4612 Fragments arranged by size: 4612 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3116 3307 3116 Size: 191 5966 PshAI GACnn'nnGTC Cuts at: 1613 2204 1613 Size: 591 5566 PsiI TTA'TAA Cuts at: 3727 3727 Size: 6157 PstI C_TGCA'G Cuts at: 830 2799 3509 830 Size: 1969 710 3478 Fragments arranged by size: 3478 1969 710 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6157 PvuII CAG'CTG Cuts at: 2842 3028 3154 2842 Size: 186 126 5845 Fragments arranged by size: 5845 186 126 SacI G_AGCT'C Cuts at: 3233 3233 Size: 6157 SalI G'TCGA_C Cuts at: 1936 3499 1936 Size: 1563 4594 SbfI CC_TGCA'GG Cuts at: 3509 3509 Size: 6157 ScaI AGT'ACT Cuts at: 4405 6117 4405 Size: 1712 4445 SfcI C'TryA_G Cuts at: 826 1045 2795 3505 4640 5321 5512 826 Size: 219 1750 710 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 710 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6157 SmaI CCC'GGG Cuts at: 1872 2029 2924 3135 3484 1872 Size: 157 895 211 349 4545 Fragments arranged by size: 4545 895 349 211 157 SmlI C'TyrA_G Cuts at: 820 1017 1943 3188 4261 5162 5424 820 Size: 197 926 1245 1073 901 262 1553 Fragments arranged by size: 1553 1245 1073 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3948 493 Size: 3455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6157 SphI G_CATG'C Cuts at: 1132 3515 1132 Size: 2383 3774 SspI AAT'ATT Cuts at: 5 52 4081 5 Size: 47 4029 2081 Fragments arranged by size: 4029 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6157 StyI C'CwwG_G Cuts at: 513 1065 3437 3444 3596 513 Size: 552 2372 7 152 3074 Fragments arranged by size: 3074 2372 552 152 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2847 3105 4184 4201 4354 4539 4984 Size: 1255 1267 258 1079 17 153 185 445 Cuts at: 4984 325 Size: 1498 Fragments arranged by size: 1498 1267 1255 1079 445 258 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 4403 6115 Size: 274 80 33 51 1443 321 2105 1712 Cuts at: 6115 96 Size: 138 Fragments arranged by size: 2105 1712 1443 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2219 2251 6117 6149 2219 Size: 32 3866 32 2227 Fragments arranged by size: 3866 2227 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6157 XbaI T'CTAG_A Cuts at: 3493 3493 Size: 6157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2854 3095 2539 Size: 315 241 5601 Fragments arranged by size: 5601 315 241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6157 XmaI C'CCGG_G Cuts at: 1870 2027 2922 3133 3482 1870 Size: 157 895 211 349 4545 Fragments arranged by size: 4545 895 349 211 157 XmnI GAAnn'nnTTC Cuts at: 2505 4286 2505 Size: 1781 4376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 3965 276 Size: 53 83 186 936 561 1870 2468 Fragments arranged by size: 2468 1870 936 561 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI