################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI DraI DraIII Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI MamI MfeI MluI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SalI SapI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 1314 GGTACC G^GTACC AccI 259 GTCTAC GT^[AC][GT]AC AccIII 38 TCCGGA T^CCGGA AcsI 1389 GAATTT [GA]^AATT[CT] AcyI 13 GGCGCC G[GA]^CG[CT]C AocI 1973 CCTTAGG CC^T[ACGT]AGG ApaI 913 GGGCCC GGGCC^C ApoI 1389 GAATTT [GA]^AATT[CT] Asp700I 1225 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1314 GGTACC G^GTACC AspI 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2336 TGGCCA TGG^CCA BamHI 164 GGATCC G^GATCC BbeI 13 GGCGCC GGCGC^C BcgI 2352 CGATGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 565 CTAG C^TAG BsaAI 723 CACGTA [CT]AC^GT[GA] BsaHI 13 GGCGCC G[GA]^CG[CT]C BseAI 38 TCCGGA T^CCGGA BspEI 38 TCCGGA T^CCGGA BspHI 1421 TCATGA T^CATGA BssSI 1135 CACGAG CACGAG(-5/-1) BstEII 2089 GGTGACC G^GT[ACGT]ACC BstPI 2089 GGTGACC G^GT[ACGT]ACC Bsu36I 1973 CCTTAGG CC^T[ACGT]AGG CpoI 40 CGGACCG CG^G[AT]CCG DrdI 1618 GACTCACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1973 CCTTAGG CC^T[ACGT]AGG EcoO65I 2089 GGTGACC G^GT[ACGT]ACC EcoT14I 1880 CCAAGG C^C[AT][AT]GG Hin1I 13 GGCGCC G[GA]^CG[CT]C HindIII 1195 AAGCTT A^AGCTT HpaI 1171 GTTAAC GTT^AAC KasI 13 GGCGCC G^GCGCC KpnI 1314 GGTACC GGTAC^C KspI 135 CCGCGG CCGC^GG MaeI 565 CTAG C^TAG MluNI 2336 TGGCCA TGG^CCA MroI 38 TCCGGA T^CCGGA MscI 2336 TGGCCA TGG^CCA NarI 13 GGCGCC GG^CGCC PaeR7I 1078 CTCGAG C^TCGAG PflFI 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2034 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1421 TCATGA T^CATGA RsrII 40 CGGACCG CG^G[AT]CCG SacII 135 CCGCGG CCGC^GG ScaI 2218 AGTACT AGT^ACT SfiI 133 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 13 GGCGCC GGC^GCC SspI 1742 AATATT AAT^ATT StyI 1880 CCAAGG C^C[AT][AT]GG Tth111I 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1078 CTCGAG C^TCGAG XmnI 1225 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 136 CGCG CG^CG 2299 CGCG AvaI 1078 CTCGAG C^[CT]CG[GA]G 2214 CCCGAG BbvCI 900 GCTGAGG CCTCAGC(-5/-2) 2124 GCTGAGG BsaWI 38 TCCGGA [AT]^CCGG[AT] 1769 ACCGGA BseRI 435 GAGGAG GAGGAG(10/8) 942 GAGGAG BsoBI 1078 CTCGAG C^[CT]CG[GA]G 2214 CCCGAG BspMI 174 GCAGGT ACCTGC(4/8) 807 GCAGGT BsrDI 1436 CATTGC GCAATG(2/0) 1647 CATTGC BstAPI 525 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2347 GCAGGCGATGC BstUI 136 CGCG CG^CG 2299 CGCG BstYI 59 AGATCC [GA]^GATC[CT] 164 GGATCC EarI 371 GAAGAG CTCTTC(1/4) 1164 GAAGAG HincII 1171 GTTAAC GT[CT]^[GA]AC 2470 GTCAAC HindII 1171 GTTAAC GT[CT]^[GA]AC 2470 GTCAAC HphI 2089 GGTGA GGTGA(8/7) 231 TCACC Ksp632I 371 GAAGAG CTCTTC(1/4) 1164 GAAGAG MflI 59 AGATCC [GA]^GATC[CT] 164 GGATCC MslI 1044 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2083 CATGTTGGTG MvnI 136 CGCG CG^CG 2299 CGCG NotI 131 GCGGCCGC GC^GGCCGC 970 GCGGCCGC PpuMI 415 AGGTCCT [GA]G^G[AT]CC[CT] 859 AGGTCCT XhoII 59 AGATCC [GA]^GATC[CT] 164 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI