(Linear) (Six-base) MAPSORT of: KIBB8524.seq Check: 400 from: 1 to: 2511 KIBB8524.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 165 2511 Size: 165 2346 AccI GT'mk_AC Cuts at: 0 260 2511 Size: 260 2251 Acc65I G'GTAC_C Cuts at: 0 1314 2511 Size: 1314 1197 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 1175 1357 1505 2511 Size: 133 1042 182 148 1006 Fragments arranged by size: 1042 1006 182 148 133 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 838 870 2511 Size: 838 32 1641 Fragments arranged by size: 1641 838 32 AlwNI CAG_nnn'CTG Cuts at: 0 63 1335 2248 2511 Size: 63 1272 913 263 Fragments arranged by size: 1272 913 263 63 ApaI G_GGCC'C Cuts at: 0 917 2511 Size: 917 1594 ApoI r'AATT_y Cuts at: 0 1389 2511 Size: 1389 1122 AvaI C'yCGr_G Cuts at: 0 1078 2214 2511 Size: 1078 1136 297 Fragments arranged by size: 1136 1078 297 BamHI G'GATC_C Cuts at: 0 164 2511 Size: 164 2347 BanI G'GyrC_C Cuts at: 0 13 616 822 1314 2511 Size: 13 603 206 492 1197 Fragments arranged by size: 1197 603 492 206 13 BanII G_rGCy'C Cuts at: 0 477 797 880 917 1733 1983 2511 Size: 477 320 83 37 816 250 528 Fragments arranged by size: 816 528 477 320 250 83 37 BbeI G_GCGC'C Cuts at: 0 17 2511 Size: 17 2494 BbsI GAAGACnn'nnnn_ Cuts at: 0 1232 1280 2464 2511 Size: 1232 48 1184 47 Fragments arranged by size: 1232 1184 48 47 BbvCI CC'TCA_GC Cuts at: 0 901 2125 2511 Size: 901 1224 386 Fragments arranged by size: 1224 901 386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2341 2375 2511 Size: 2341 34 136 Fragments arranged by size: 2341 136 34 BglI GCCn_nnn'nGGC Cuts at: 0 140 614 712 2511 Size: 140 474 98 1799 Fragments arranged by size: 1799 474 140 98 Bme1580I G_kGCm'C Cuts at: 0 527 827 917 2415 2511 Size: 527 300 90 1498 96 Fragments arranged by size: 1498 527 300 96 90 BmgBI CAC'GTC Cuts at: 0 1851 2511 Size: 1851 660 BmrI ACTGGGnnnn_n' Cuts at: 0 911 2511 Size: 911 1600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 446 599 2511 Size: 446 153 1912 Fragments arranged by size: 1912 446 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 463 1078 1312 1741 2511 Size: 66 397 615 234 429 770 Fragments arranged by size: 770 615 429 397 234 66 Bpu10I CC'TnA_GC Cuts at: 0 901 2125 2511 Size: 901 1224 386 Fragments arranged by size: 1224 901 386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 441 1177 1275 1396 2511 Size: 441 736 98 121 1115 Fragments arranged by size: 1115 736 441 121 98 BsaAI yAC'GTr Cuts at: 0 725 2511 Size: 725 1786 BsaHI Gr'CG_yC Cuts at: 0 14 2511 Size: 14 2497 BsaWI w'CCGG_w Cuts at: 0 38 1769 2511 Size: 38 1731 742 Fragments arranged by size: 1731 742 38 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1882 1912 2511 Size: 1882 30 599 Fragments arranged by size: 1882 599 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 450 957 2511 Size: 450 507 1554 Fragments arranged by size: 1554 507 450 BseYI C'CCAG_C Cuts at: 0 18 365 520 910 1025 1029 2511 Size: 18 347 155 390 115 4 1482 Fragments arranged by size: 1482 390 347 155 115 18 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 517 2366 2511 Size: 199 318 1849 145 Fragments arranged by size: 1849 318 199 145 BsiEI CG_ry'CG Cuts at: 0 135 974 2000 2511 Size: 135 839 1026 511 Fragments arranged by size: 1026 839 511 135 BsiHKAI G_wGCw'C Cuts at: 0 880 1733 1983 2511 Size: 880 853 250 528 Fragments arranged by size: 880 853 528 250 BsmI GAATG_Cn' Cuts at: 0 1041 1694 1902 2298 2511 Size: 1041 653 208 396 213 Fragments arranged by size: 1041 653 396 213 208 Bsp1286I G_dGCh'C Cuts at: 0 477 527 797 827 880 917 1733 1983 Size: 477 50 270 30 53 37 816 250 Cuts at: 1983 2415 2511 Size: 432 96 Fragments arranged by size: 816 477 432 270 250 96 53 50 37 30 BspEI T'CCGG_A Cuts at: 0 38 2511 Size: 38 2473 BspHI T'CATG_A Cuts at: 0 1421 2511 Size: 1421 1090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 165 798 2511 Size: 165 633 1713 Fragments arranged by size: 1713 633 165 BsrDI GCAATG_nn' Cuts at: 0 1435 1646 2511 Size: 1435 211 865 Fragments arranged by size: 1435 865 211 BssSI C'ACGA_G Cuts at: 0 1135 2511 Size: 1135 1376 BstAPI GCAn_nnn'nTGC Cuts at: 0 531 2353 2511 Size: 531 1822 158 Fragments arranged by size: 1822 531 158 BstEII G'GTnAC_C Cuts at: 0 2089 2511 Size: 2089 422 BstXI CCAn_nnnn'nTGG Cuts at: 0 1183 1353 1887 2511 Size: 1183 170 534 624 Fragments arranged by size: 1183 624 534 170 BstYI r'GATC_y Cuts at: 0 59 164 2511 Size: 59 105 2347 Fragments arranged by size: 2347 105 59 Bsu36I CC'TnA_GG Cuts at: 0 1974 2511 Size: 1974 537 BtgI C'CryG_G Cuts at: 0 135 212 404 926 1676 2511 Size: 135 77 192 522 750 835 Fragments arranged by size: 835 750 522 192 135 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1606 2366 2511 Size: 1606 760 145 Fragments arranged by size: 1606 760 145 BtsI GCAGTG_nn' Cuts at: 0 249 1073 2511 Size: 249 824 1438 Fragments arranged by size: 1438 824 249 DrdI GACnn_nn'nnGTC Cuts at: 0 1624 2511 Size: 1624 887 EaeI y'GGCC_r Cuts at: 0 132 141 963 971 1997 2336 2511 Size: 132 9 822 8 1026 339 175 Fragments arranged by size: 1026 822 339 175 132 9 8 EagI C'GGCC_G Cuts at: 0 132 971 1997 2511 Size: 132 839 1026 514 Fragments arranged by size: 1026 839 514 132 EarI CTCTTCn'nnn_ Cuts at: 0 366 1159 2511 Size: 366 793 1352 Fragments arranged by size: 1352 793 366 EcoICRI GAG'CTC Cuts at: 0 878 1731 1981 2511 Size: 878 853 250 530 Fragments arranged by size: 878 853 530 250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 66 133 463 1078 1175 1312 1357 1505 Size: 66 67 330 615 97 137 45 148 Cuts at: 1505 1741 2511 Size: 236 770 Fragments arranged by size: 770 615 330 236 148 137 97 67 66 45 EcoO109I rG'GnC_Cy Cuts at: 0 416 860 1250 2511 Size: 416 444 390 1261 Fragments arranged by size: 1261 444 416 390 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1456 2511 Size: 1456 1055 HaeII r_GCGC'y Cuts at: 0 17 94 636 1577 2511 Size: 17 77 542 941 934 Fragments arranged by size: 941 934 542 77 17 HincII GTy'rAC Cuts at: 0 1173 2472 2511 Size: 1173 1299 39 Fragments arranged by size: 1299 1173 39 HindIII A'AGCT_T Cuts at: 0 1195 2511 Size: 1195 1316 HpaI GTT'AAC Cuts at: 0 1173 2511 Size: 1173 1338 KasI G'GCGC_C Cuts at: 0 13 2511 Size: 13 2498 KpnI G_GTAC'C Cuts at: 0 1318 2511 Size: 1318 1193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 273 2390 2511 Size: 273 2117 121 Fragments arranged by size: 2117 273 121 MscI TGG'CCA Cuts at: 0 2338 2511 Size: 2338 173 MslI CAynn'nnrTG Cuts at: 0 1048 2087 2511 Size: 1048 1039 424 Fragments arranged by size: 1048 1039 424 MspA1I CmG'CkG Cuts at: 0 22 131 137 819 938 1029 1719 2511 Size: 22 109 6 682 119 91 690 792 Fragments arranged by size: 792 690 682 119 109 91 22 6 NarI GG'CG_CC Cuts at: 0 14 2511 Size: 14 2497 NotI GC'GGCC_GC Cuts at: 0 132 971 2511 Size: 132 839 1540 Fragments arranged by size: 1540 839 132 NspI r_CATG'y Cuts at: 0 330 1095 2086 2511 Size: 330 765 991 425 Fragments arranged by size: 991 765 425 330 PfoI T'CCnGG_A Cuts at: 0 167 1102 2137 2511 Size: 167 935 1035 374 Fragments arranged by size: 1035 935 374 167 PpuMI rG'GwC_Cy Cuts at: 0 416 860 2511 Size: 416 444 1651 Fragments arranged by size: 1651 444 416 PshAI GACnn'nnGTC Cuts at: 0 2038 2511 Size: 2038 473 PsiI TTA'TAA Cuts at: 0 1915 2511 Size: 1915 596 PspOMI G'GGCC_C Cuts at: 0 913 2511 Size: 913 1598 PspXI vC'TCGA_Gb Cuts at: 0 1078 2511 Size: 1078 1433 PstI C_TGCA'G Cuts at: 0 833 1119 1205 1988 2063 2156 2511 Size: 833 286 86 783 75 93 355 Fragments arranged by size: 833 783 355 286 93 86 75 PvuII CAG'CTG Cuts at: 0 22 1029 1719 2511 Size: 22 1007 690 792 Fragments arranged by size: 1007 792 690 22 RsrII CG'GwC_CG Cuts at: 0 41 2511 Size: 41 2470 SacI G_AGCT'C Cuts at: 0 880 1733 1983 2511 Size: 880 853 250 528 Fragments arranged by size: 880 853 528 250 SacII CC_GC'GG Cuts at: 0 138 2511 Size: 138 2373 ScaI AGT'ACT Cuts at: 0 2220 2511 Size: 2220 291 SfcI C'TryA_G Cuts at: 0 829 1115 1201 1984 2059 2152 2240 2511 Size: 829 286 86 783 75 93 88 271 Fragments arranged by size: 829 783 286 271 93 88 86 75 SfiI GGCCn_nnn'nGGCC Cuts at: 0 140 2511 Size: 140 2371 SfoI GGC'GCC Cuts at: 0 15 2511 Size: 15 2496 SmlI C'TyrA_G Cuts at: 0 420 1078 1192 1254 1411 2511 Size: 420 658 114 62 157 1100 Fragments arranged by size: 1100 658 420 157 114 62 SspI AAT'ATT Cuts at: 0 1744 2511 Size: 1744 767 StyI C'CwwG_G Cuts at: 0 1880 2511 Size: 1880 631 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1466 2511 Size: 1466 1045 TatI w'GTAC_w Cuts at: 0 386 500 2218 2511 Size: 386 114 1718 293 Fragments arranged by size: 1718 386 293 114 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1882 1914 2511 Size: 1882 32 597 Fragments arranged by size: 1882 597 32 Tth111I GACn'n_nGTC Cuts at: 0 2209 2511 Size: 2209 302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 114 750 924 2050 2511 Size: 114 636 174 1126 461 Fragments arranged by size: 1126 636 461 174 114 XhoI C'TCGA_G Cuts at: 0 1078 2511 Size: 1078 1433 XmnI GAAnn'nnTTC Cuts at: 0 1229 2511 Size: 1229 1282 Enzymes that do cut: AarI AccI Acc65I AcuI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII HincII HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I NarI NotI NspI PfoI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII RsrII SacI SacII ScaI SfcI SfiI SfoI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BlpI BmtI BsaI BsaBI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsrI PvuI SalI SanDI SapI SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XmaI ZraI