################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AciI 627 GCGG CCGC(-3/-1) AcsI 137 AAATTC [GA]^AATT[CT] AfaI 778 GTAC GT^AC ApaI 403 GGGCCC GGGCC^C ApoI 137 AAATTC [GA]^AATT[CT] Asp700I 273 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 666 GGTCC G^G[AT]CC BanII 403 GGGCCC G[GA]GC[CT]^C BbvI 295 GCTGC GCAGC(8/12) BciVI 810 GTATCC GTATCC(6/5) BglII 119 AGATCT A^GATCT BlpI 130 GCTGAGC GC^T[ACGT]AGC BmyI 403 GGGCCC G[AGT]GC[ACT]^C BpmI 159 CTCCAG CTGGAG(16/14) Bpu1102I 130 GCTGAGC GC^T[ACGT]AGC BsaI 156 GGTCTC GGTCTC(1/5) BseRI 123 CTCCTC GAGGAG(10/8) BsiYI 834 CCTGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 834 CCTGATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 157 GTCTC GTCTC(1/5) BsmI 507 GAATGC GAATGC(1/-1) Bsp1286I 403 GGGCCC G[AGT]GC[ACT]^C BsrI 101 ACTGG ACTGG(1/-1) BssKI 260 CCTGG ^CC[ACGT]GG BstNI 260 CCTGG CC^[AT]GG CelII 130 GCTGAGC GC^T[ACGT]AGC DsaI 443 CCATGG C^C[GA][CT]GG EarI 366 GAAGAG CTCTTC(1/4) EcoRII 260 CCTGG ^CC[AT]GG HaeIII 404 GGCC GG^CC HincII 106 GTTAAC GT[CT]^[GA]AC HindII 106 GTTAAC GT[CT]^[GA]AC HinfI 750 GAATC G^A[ACGT]TC HpaI 106 GTTAAC GTT^AAC HphI 390 GGTGA GGTGA(8/7) Ksp632I 366 GAAGAG CTCTTC(1/4) MspA1I 128 CAGCTG C[AC]G^C[GT]G MvaI 260 CCTGG CC^[AT]GG MwoI 250 GCTTCTAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 443 CCATGG C^CATGG NheI 298 GCTAGC G^CTAGC NspI 284 GCATGT [GA]CATG^[CT] PvuII 128 CAGCTG CAG^CTG RsaI 778 GTAC GT^AC ScrFI 260 CCTGG CC^[ACGT]GG SfaNI 624 GATGC GCATC(5/9) TfiI 750 GAATC G^A[AT]TC TseI 295 GCTGC G^C[AT]GC TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 273 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AlwI 652 GATCC GGATC(4/5) 643 GGATC BbvCI 125 CCTCAGC CCTCAGC(-5/-2) 303 CCTCAGC BstF5I 831 CATCC GGATG(2/0) 447 GGATG Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC 298 GCTAGC DraII 402 GGGGCCC [GA]G^G[ACGT]CC[CT] 403 GGGCCCT EcoO109I 402 GGGGCCC [GA]G^G[ACGT]CC[CT] 403 GGGCCCT Fnu4HI 295 GCTGC GC^[ACGT]GC 627 GCGGC FokI 831 CATCC GGATG(9/13) 447 GGATG HindIII 525 AAGCTT A^AGCTT 677 AAGCTT ItaI 295 GCTGC GC^[ACGT]GC 627 GCGGC Tsp45I 484 GTGAC ^GT[GC]AC 691 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BfaI BsaJI BstYI Cfr13I DdeI DpnII EcoT14I MaeI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII NlaIV Sau3AI Sau96I SmlI StyI Tru9I Tsp509I XhoII