################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FokI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AflIII 443 ACACGT A^C[GA][CT]GT AlwNI 282 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG BclI 132 TGATCA T^GATCA BglII 119 AGATCT A^GATCT BsaAI 444 CACGTA [CT]AC^GT[GA] BseRI 123 CTCCTC GAGGAG(10/8) BsmI 507 GAATGC GAATGC(1/-1) BspMI 885 GCAGGT ACCTGC(4/8) BsrI 915 CCAGT ACTGG(1/-1) BstXI 792 CCAAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 231 TTTAAA TTT^AAA DraIII 427 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 633 CCATGG C^C[GA][CT]GG EcoRV 576 GATATC GAT^ATC EcoT14I 633 CCATGG C^C[AT][AT]GG FbaI 132 TGATCA T^GATCA HgaI 852 GACGC GACGC(5/10) HincII 918 GTCAAC GT[CT]^[GA]AC HindII 918 GTCAAC GT[CT]^[GA]AC HindIII 794 AAGCTT A^AGCTT MspA1I 128 CAGCTG C[AC]G^C[GT]G NcoI 633 CCATGG C^CATGG NheI 543 GCTAGC G^CTAGC PvuII 128 CAGCTG CAG^CTG SfcI 533 CTGTAG C^T[GA][CT]AG SspI 183 AATATT AAT^ATT StyI 633 CCATGG C^C[AT][AT]GG TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 29 CCATTCTAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvCI 125 CCTCAGC CCTCAGC(-5/-2) 303 CCTCAGC BbvI 627 GCAGC GCAGC(8/12) 145 GCTGC BpmI 159 CTCCAG CTGGAG(16/14) 339 CTCCAG BsmAI 111 GAGAC GTCTC(1/5) 157 GTCTC BsrDI 96 CATTGC GCAATG(2/0) 511 GCAATG BssKI 101 CCTGG ^CC[ACGT]GG 260 CCTGG BstNI 101 CCTGG CC^[AT]GG 260 CCTGG Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC 543 GCTAGC EcoRII 101 CCTGG ^CC[AT]GG 260 CCTGG Fnu4HI 145 GCTGC GC^[ACGT]GC 627 GCAGC HinfI 213 GAATC G^A[ACGT]TC 803 GAATC ItaI 145 GCTGC GC^[ACGT]GC 627 GCAGC MaeII 445 ACGT A^CGT 482 ACGT MvaI 101 CCTGG CC^[AT]GG 260 CCTGG MwoI 145 GCTGCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 538 GCCATGCTAGC NlaIII 540 CATG CATG^ 634 CATG ScrFI 101 CCTGG CC^[ACGT]GG 260 CCTGG SmlI 322 CTCAAG C^T[CT][GA]AG 647 CTCAAG TfiI 213 GAATC G^A[AT]TC 803 GAATC TseI 145 GCTGC G^C[AT]GC 627 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BsaJI BstYI DdeI DpnII HphI MaeI MaeIII MboI MboII MflI MnlI MseI NdeII Sau3AI SfaNI Tru9I Tsp45I Tsp509I XhoII