(Circular) (Six-base) MAPSORT of: pFN21AE5458.seq Check: 1662 from: 1 to: 5656 pFN21AE5458 5656 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 5103 5371 1632 Size: 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2904 4555 5532 1203 Size: 1701 1651 977 1327 Fragments arranged by size: 1701 1651 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2835 1166 Size: 1669 3987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2654 1184 Size: 1470 4186 AlwNI CAG_nnn'CTG Cuts at: 3399 4971 3399 Size: 1572 4084 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2051 2065 2939 2965 3191 3343 5452 1493 Size: 558 14 874 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 558 226 152 26 14 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2142 2986 3353 2142 Size: 844 367 4445 Fragments arranged by size: 4445 844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2977 4432 618 Size: 325 217 627 1190 1455 1842 Fragments arranged by size: 1842 1455 1190 627 325 217 BanII G_rGCy'C Cuts at: 1391 2925 2975 1391 Size: 1534 50 4072 Fragments arranged by size: 4072 1534 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2413 928 Size: 971 514 4171 Fragments arranged by size: 4171 971 514 BbvCI CC'TCA_GC Cuts at: 2897 2897 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2471 3548 4758 2471 Size: 1077 1210 3369 Fragments arranged by size: 3369 1210 1077 BclI T'GATC_A Cuts at: 1548 2277 2703 1548 Size: 729 426 4501 Fragments arranged by size: 4501 729 426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2124 5651 2124 Size: 3527 2129 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 3717 4873 1790 Size: 1927 1156 2573 Fragments arranged by size: 2573 1927 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2116 3387 4345 Size: 416 53 83 399 24 699 1271 958 Cuts at: 4345 442 Size: 1753 Fragments arranged by size: 1753 1271 958 699 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4316 1239 Size: 3077 2579 Bpu10I CC'TnA_GC Cuts at: 2897 2897 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 3781 4646 4944 2315 Size: 1466 865 298 3027 Fragments arranged by size: 3027 1466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 2450 5650 2450 Size: 3200 2456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2667 3463 3845 4480 Size: 53 83 186 936 1134 796 382 635 Cuts at: 4480 275 Size: 1451 Fragments arranged by size: 1451 1134 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2660 4089 4487 4761 4908 5319 2660 Size: 1429 398 274 147 411 2997 Fragments arranged by size: 2997 1429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 2703 1186 Size: 1408 109 4139 Fragments arranged by size: 4139 1408 109 BseYI C'CCAG_C Cuts at: 2221 4859 5355 2221 Size: 2638 496 2522 Fragments arranged by size: 2638 2522 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3717 3802 4873 3717 Size: 85 1071 4500 Fragments arranged by size: 4500 1071 85 BsmI GAATG_Cn' Cuts at: 2259 3167 3260 2259 Size: 908 93 4655 Fragments arranged by size: 4655 908 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2658 2658 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2925 2975 3717 3802 4873 1391 Size: 399 1135 50 742 85 1071 2174 Fragments arranged by size: 2174 1135 1071 742 399 85 50 BspEI T'CCGG_A Cuts at: 2660 5319 2660 Size: 2659 2997 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2598 4151 4325 65 Size: 1183 1350 1553 174 1396 Fragments arranged by size: 1553 1396 1350 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2124 2142 2986 3353 3738 3755 5207 5293 Size: 152 18 844 367 385 17 1452 86 Cuts at: 5293 5305 5651 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 844 385 367 346 152 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2926 2926 Size: 5656 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2612 487 Size: 683 56 1386 3531 Fragments arranged by size: 3531 1386 683 56 BtsI GCAGTG_nn' Cuts at: 2148 3172 3966 3986 2148 Size: 1024 794 20 3818 Fragments arranged by size: 3818 1024 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2433 2960 2433 Size: 527 5129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621 Size: 54 1425 50 275 18 1273 889 1629 Cuts at: 5621 8 Size: 43 Fragments arranged by size: 1629 1425 1273 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2134 3586 1353 Size: 781 1452 3423 Fragments arranged by size: 3423 1452 781 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3719 4316 5103 5371 1239 Size: 393 2087 597 787 268 1524 Fragments arranged by size: 2087 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2767 2767 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2754 4770 4954 1305 Size: 1449 2016 184 2007 Fragments arranged by size: 2016 2007 1449 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2289 2460 2835 3614 3973 4132 518 Size: 648 1123 171 375 779 359 159 2042 Fragments arranged by size: 2042 1123 779 648 375 359 171 159 MspA1I CmG'CkG Cuts at: 1606 3072 3749 4897 5142 1606 Size: 1466 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2908 3014 4559 1132 Size: 97 1679 106 1545 2229 Fragments arranged by size: 2229 1679 1545 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 2904 4555 2904 Size: 1651 4005 PflMI CCAn_nnn'nTGG Cuts at: 1108 2806 1108 Size: 1698 3958 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2417 1613 Size: 804 4852 PsiI TTA'TAA Cuts at: 2057 3226 2057 Size: 1169 4487 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2304 3004 4139 4820 5011 826 Size: 219 1259 700 1135 681 191 1471 Fragments arranged by size: 1471 1259 1135 700 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 3760 4661 4923 820 Size: 197 926 387 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 387 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 2190 2437 3580 5 Size: 47 2138 247 1143 2081 Fragments arranged by size: 2138 2081 1143 247 47 StuI AGG'CCT Cuts at: 1517 2310 2873 1517 Size: 793 563 4300 Fragments arranged by size: 4300 793 563 StyI C'CwwG_G Cuts at: 513 1065 2004 3095 513 Size: 552 939 1091 3074 Fragments arranged by size: 3074 1091 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2407 3683 3700 3853 4038 4483 Size: 1255 417 410 1276 17 153 185 445 Cuts at: 4483 325 Size: 1498 Fragments arranged by size: 1498 1276 1255 445 417 410 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 2108 1316 Size: 792 4864 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI