################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 60 CGCG CG^CG AcyI 3 GGCGTC G[GA]^CG[CT]C AflII 235 CTTAAG C^TTAAG Aor51HI 34 AGCGCT AGC^GCT AvaI 116 CTCGGG C^[CT]CG[GA]G AvaII 54 GGTCC G^G[AT]CC AviII 101 TGCGCA TGC^GCA BalI 170 TGGCCA TGG^CCA BamHI 273 GGATCC G^GATCC BcnI 87 CCCGG CC^[GC]GG BfrI 235 CTTAAG C^TTAAG BglI 290 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 37 GCTGAGC GC^T[ACGT]AGC Bpu1102I 37 GCTGAGC GC^T[ACGT]AGC BsaHI 3 GGCGTC G[GA]^CG[CT]C BsmI 93 GAATGC GAATGC(1/-1) BsoBI 116 CTCGGG C^[CT]CG[GA]G BsrFI 21 ACCGGC [GA]^CCGG[CT] BstAPI 153 GCAATTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 60 CGCG CG^CG CelII 37 GCTGAGC GC^T[ACGT]AGC Cfr10I 21 ACCGGC [GA]^CCGG[CT] Eco47III 34 AGCGCT AGC^GCT EcoNI 57 CCTCGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 342 CCATGG C^C[AT][AT]GG FspI 101 TGCGCA TGC^GCA HgaI 4 GCGTC GACGC(5/10) Hin1I 3 GGCGTC G[GA]^CG[CT]C HphI 66 GGTGA GGTGA(8/7) MluNI 170 TGGCCA TGG^CCA MscI 170 TGGCCA TGG^CCA MseI 236 TTAA T^TAA MspA1I 213 CAGCTG C[AC]G^C[GT]G MvnI 60 CGCG CG^CG NciI 87 CCCGG CC^[GC]GG NcoI 342 CCATGG C^CATGG NlaIV 273 GGATCC GG[ACGT]^[ACGT]CC PflMI 337 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 213 CAGCTG CAG^CTG SfaNI 147 GATGC GCATC(5/9) StyI 342 CCATGG C^C[AT][AT]GG TaqI 13 TCGA T^CGA Tru9I 236 TTAA T^TAA Tsp509I 155 AATT ^AATT TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 13 TCGA T^CGA Van91I 337 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 214 AGCT AG^CT 302 AGCT BstNI 72 CCAGG CC^[AT]GG 270 CCAGG BstYI 179 AGATCC [GA]^GATC[CT] 273 GGATCC Cfr13I 54 GGTCC G^G[ACGT]CC 346 GGGCC DdeI 38 CTGAG C^T[ACGT]AG 278 CTGAG DsaI 167 CCGTGG C^C[GA][CT]GG 342 CCATGG EaeI 170 TGGCCA [CT]^GGCC[GA] 368 TGGCCG EcoRII 72 CCAGG ^CC[AT]GG 270 CCAGG HaeII 34 AGCGCT [GA]GCGC^[CT] 309 GGCGCT HapII 22 CCGG C^CGG 88 CCGG HpaII 22 CCGG C^CGG 88 CCGG MflI 179 AGATCC [GA]^GATC[CT] 273 GGATCC MspI 22 CCGG C^CGG 88 CCGG MvaI 72 CCAGG CC^[AT]GG 270 CCAGG Sau96I 54 GGTCC G^G[ACGT]CC 346 GGGCC SmlI 30 CTTGAG C^T[CT][GA]AG 235 CTTAAG XhoII 179 AGATCC [GA]^GATC[CT] 273 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AlwI BbvI BsaJI BsiYI BslI BssKI Cac8I CfoI DpnII Fnu4HI HaeIII HhaI HinP1I ItaI MboI MnlI MwoI NdeII NlaIII Sau3AI ScrFI TseI