################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AatII 886 GACGTC GACGT^C AciI 363 CCGC CCGC(-3/-1) AcsI 12 GAATTC [GA]^AATT[CT] AcyI 886 GACGTC G[GA]^CG[CT]C AflIII 530 ACATGT A^C[GA][CT]GT AlwI 72 GATCC GGATC(4/5) AlwNI 714 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 873 CCTGAGG CC^T[ACGT]AGG ApoI 12 GAATTC [GA]^AATT[CT] AseI 861 ATTAAT AT^TAAT AsnI 861 ATTAAT AT^TAAT AvaII 701 GGTCC G^G[AT]CC BalI 371 TGGCCA TGG^CCA BanI 843 GGTGCC G^G[CT][GA]CC BbsI 819 GTCTTC GAAGAC(2/6) BcnI 61 CCGGG CC^[GC]GG BfaI 94 CTAG C^TAG BpmI 666 CTCCAG CTGGAG(16/14) BpuAI 819 GTCTTC GAAGAC(2/6) BsaAI 103 TACGTG [CT]AC^GT[GA] BsaHI 886 GACGTC G[GA]^CG[CT]C Bsp1407I 392 TGTACA T^GTACA BspLU11I 530 ACATGT A^CATGT BsrDI 349 GCAATG GCAATG(2/0) BsrGI 392 TGTACA T^GTACA BsrI 221 CCAGT ACTGG(1/-1) BstEII 399 GGTCACC G^GT[ACGT]ACC BstPI 399 GGTCACC G^GT[ACGT]ACC BstYI 71 AGATCC [GA]^GATC[CT] Bsu36I 873 CCTGAGG CC^T[ACGT]AGG DpnII 72 GATC ^GATC DraIII 477 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 371 TGGCCA [CT]^GGCC[GA] Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoO65I 399 GGTCACC G^GT[ACGT]ACC EcoRI 12 GAATTC G^AATTC EcoT14I 467 CCATGG C^C[AT][AT]GG EcoT22I 923 ATGCAT ATGCA^T HapII 61 CCGG C^CGG Hin1I 886 GACGTC G[GA]^CG[CT]C HincII 520 GTCAAC GT[CT]^[GA]AC HindII 520 GTCAAC GT[CT]^[GA]AC HpaII 61 CCGG C^CGG MaeI 94 CTAG C^TAG MboI 72 GATC ^GATC MflI 71 AGATCC [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspI 61 CCGG C^CGG NciI 61 CCGGG CC^[GC]GG NcoI 467 CCATGG C^CATGG NdeII 72 GATC ^GATC NsiI 923 ATGCAT ATGCA^T NspI 530 ACATGT [GA]CATG^[CT] PshBI 861 ATTAAT AT^TAAT PstI 720 CTGCAG CTGCA^G SapI 300 GCTCTTC GCTCTTC(1/4) Sau3AI 72 GATC ^GATC SfcI 720 CTGCAG C^T[GA][CT]AG SmlI 553 CTTGAG C^T[CT][GA]AG SspBI 392 TGTACA T^GTACA StyI 467 CCATGG C^C[AT][AT]GG TfiI 685 GATTC G^A[AT]TC XhoII 71 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BbvI 722 GCAGC GCAGC(8/12) 4 GCTGC BmyI 625 GTGCCC G[AGT]GC[ACT]^C 844 GTGCCC BsmAI 325 GTCTC GTCTC(1/5) 408 GTCTC BsmI 766 GCATTC GAATGC(1/-1) 925 GCATTC Bsp1286I 625 GTGCCC G[AGT]GC[ACT]^C 844 GTGCCC BspMI 718 ACCTGC ACCTGC(4/8) 698 GCAGGT DsaI 467 CCATGG C^C[GA][CT]GG 689 CCACGG EarI 79 CTCTTC CTCTTC(1/4) 301 CTCTTC Fnu4HI 4 GCTGC GC^[ACGT]GC 722 GCAGC HphI 401 TCACC GGTGA(8/7) 635 TCACC ItaI 4 GCTGC GC^[ACGT]GC 722 GCAGC Ksp632I 79 CTCTTC CTCTTC(1/4) 301 CTCTTC MaeII 104 ACGT A^CGT 887 ACGT MwoI 472 GCCCACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 716 GCACCTGCAGC SfaNI 272 GCATC GCATC(5/9) 671 GCATC TseI 4 GCTGC G^C[AT]GC 722 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FokI HaeIII HinfI MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV PleI RsaI Sau96I ScrFI Tru9I Tsp45I Tsp509I TspRI