(Linear) (Six-base) MAPSORT of: KIEE5425.seq Check: 711 from: 1 to: 933 KIEE5425.orf With 182 enzymes: SgfI * January 29, 2014 12:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 727 933 Size: 727 206 AatII G_ACGT'C Cuts at: 0 890 933 Size: 890 43 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 696 933 Size: 696 237 AflIII A'CryG_T Cuts at: 0 530 933 Size: 530 403 AleI CACnn'nnGTG Cuts at: 0 115 577 841 933 Size: 115 462 264 92 Fragments arranged by size: 462 264 115 92 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 259 291 933 Size: 259 32 642 Fragments arranged by size: 642 259 32 AlwNI CAG_nnn'CTG Cuts at: 0 719 933 Size: 719 214 ApoI r'AATT_y Cuts at: 0 12 933 Size: 12 921 AseI AT'TA_AT Cuts at: 0 862 933 Size: 862 71 BanI G'GyrC_C Cuts at: 0 843 933 Size: 843 90 BbsI GAAGACnn'nnnn_ Cuts at: 0 812 933 Size: 812 121 BfrBI ATG'CAT Cuts at: 0 925 933 Size: 925 8 Bme1580I G_kGCm'C Cuts at: 0 629 848 933 Size: 629 219 85 Fragments arranged by size: 629 219 85 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 33 933 Size: 33 900 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 651 933 Size: 651 282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 933 Size: 574 359 BsaAI yAC'GTr Cuts at: 0 105 933 Size: 105 828 BsaHI Gr'CG_yC Cuts at: 0 887 933 Size: 887 46 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 259 289 393 423 685 715 933 Size: 259 30 104 30 262 30 218 Fragments arranged by size: 262 259 218 104 30 30 30 BseYI C'CCAG_C Cuts at: 0 460 933 Size: 460 473 BsmI GAATG_Cn' Cuts at: 0 766 925 933 Size: 766 159 8 Fragments arranged by size: 766 159 8 Bsp1286I G_dGCh'C Cuts at: 0 629 848 933 Size: 629 219 85 Fragments arranged by size: 629 219 85 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 689 727 933 Size: 689 38 206 Fragments arranged by size: 689 206 38 BsrDI GCAATG_nn' Cuts at: 0 356 933 Size: 356 577 BsrGI T'GTAC_A Cuts at: 0 392 933 Size: 392 541 BstEII G'GTnAC_C Cuts at: 0 399 933 Size: 399 534 BstYI r'GATC_y Cuts at: 0 71 933 Size: 71 862 Bsu36I CC'TnA_GG Cuts at: 0 874 933 Size: 874 59 BtgI C'CryG_G Cuts at: 0 467 689 933 Size: 467 222 244 Fragments arranged by size: 467 244 222 DraIII CAC_nnn'GTG Cuts at: 0 482 933 Size: 482 451 EaeI y'GGCC_r Cuts at: 0 371 933 Size: 371 562 EarI CTCTTCn'nnn_ Cuts at: 0 85 307 933 Size: 85 222 626 Fragments arranged by size: 626 222 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 651 696 933 Size: 651 45 237 Fragments arranged by size: 651 237 45 EcoRI G'AATT_C Cuts at: 0 12 933 Size: 12 921 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 927 933 Size: 927 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 56 88 685 717 933 Size: 56 32 597 32 216 Fragments arranged by size: 597 216 56 32 32 HincII GTy'rAC Cuts at: 0 522 933 Size: 522 411 MscI TGG'CCA Cuts at: 0 373 933 Size: 373 560 MslI CAynn'nnrTG Cuts at: 0 115 172 577 841 933 Size: 115 57 405 264 92 Fragments arranged by size: 405 264 115 92 57 NcoI C'CATG_G Cuts at: 0 467 933 Size: 467 466 NsiI A_TGCA'T Cuts at: 0 927 933 Size: 927 6 NspI r_CATG'y Cuts at: 0 534 933 Size: 534 399 PciI A'CATG_T Cuts at: 0 530 933 Size: 530 403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 259 291 933 Size: 259 32 642 Fragments arranged by size: 642 259 32 PstI C_TGCA'G Cuts at: 0 724 933 Size: 724 209 SapI GCTCTTCn'nnn_ Cuts at: 0 307 933 Size: 307 626 SfcI C'TryA_G Cuts at: 0 720 933 Size: 720 213 SmlI C'TyrA_G Cuts at: 0 553 933 Size: 553 380 StyI C'CwwG_G Cuts at: 0 467 933 Size: 467 466 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 427 481 933 Size: 427 54 452 Fragments arranged by size: 452 427 54 TatI w'GTAC_w Cuts at: 0 176 392 933 Size: 176 216 541 Fragments arranged by size: 541 216 176 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 20 52 393 425 670 702 933 Size: 20 32 341 32 245 32 231 Fragments arranged by size: 341 245 231 32 32 32 20 ZraI GAC'GTC Cuts at: 0 888 933 Size: 888 45 Enzymes that do cut: AarI AatII AcuI AflIII AleI AloI AlwNI ApoI AseI BanI BbsI BfrBI Bme1580I BplI BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsmI Bsp1286I BspMI BsrDI BsrGI BstEII BstYI Bsu36I BtgI DraIII EaeI EarI Eco57MI EcoRI FalI Hin4I HincII MscI MslI NcoI NsiI NspI PciI PpiI PstI SapI SfcI SmlI StyI TaqII TatI TstI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI